The divergent sequences of rdna from s. costatum are used to design primers meeting the requirements of the rfq - pcr. seven pairs of primers are designed and designated as primer 1 ( f / r ) ~ primer 7 ( f / r ), respectively, among which primer 1 ( f / r ), primer 2 ( f / r ), primer 5 ( f / r ), primer 6 ( f / r ) showed high level of specificities to s. costatum. then, the pcr products primed by primer6 ( f / r ) are sequenced
首先以中肋骨條藻的rdna序列為設計種特異性引物的
靶區域,共設計出7對適合rfq - pcr的引物(依次命名為primer1 ( f r ) primer7 ( f r ) ) ,並用常規pcr實驗初步證明其中有4對引物( primer1 ( f r ) 、 primer2 ( f r ) 、 primer5 ( f r ) 、 primer6 ( f r ) )可作為中肋骨條藻特異性引物的候選者;然後測定了以primer6 ( f r )為引物的pcr產物的序列,序列分析表明,中肋骨條藻的pcr產物序列與其他藻的pcr產物序列差別較大,從中可設計出滿足rfq - pcr需要的taqman探針(命名為taqman6 ) ;進一步的
核酸雜交實驗表明, taqman6隻與中肋骨條藻的pcr產物雜交,不與其他藻的pcr產物雜交。
Study and primary application of the screening model for antituberculosis drugs with dna gyrase b subunit as a target
亞基為
靶點的抗結
核藥物篩選模型的建立及初步應用
After 40 hour irradiation time, about 7 ci of radioactive isotope 64cu was produced via 63cu ( n, y ) 64cu reaction. after simple disposal, the irradiated copper sample was installed in the high - intesity ion sputter source on the hi - 13 tandem accelerator. then 64cu ions extracted from the high - intesity ion sputter source and injected into the tandem accelerator, 64cu ions can be accelerated to an energy of 80 mev and formed the off - line rnb since natural
S )的熱中於通量下,經過34個半衰期輻照,通過『 u … , y )生成放射性l司位素『 cll ,然後將放射性銅
靶錐注入串列加速器強流濺射離于源中,引出mcll負離于,經刁串列加速器加速而得到能量為80mcv的離線放射性
核束「 cll叭。
Based on the simple modelling calculation, the multiplicity and angular distribution of evaporated fragments from both projectile and target spectators in high energy nucleus - nucleus collisions are described
摘要在高能
核核碰撞中,基於簡單的模型計算,描述了來自彈
核和
靶核旁觀體的蒸發碎片的多重數分佈與角分佈。
The target studied in this paper is one of the most important components in the ads. coupling the two innovative fields in the nuclear scientific and technological circles that are accelerator and sub - critical reactor. the physical function of this part is be as the neutron source, accepting the medium or high energy proton, breeding spallation reaction, arising the self - sustaining nuclear fission and generating the power
本文所研究的
靶件作為該系統最重要的關鍵部件之一,耦合
核科技界的兩大創新領域-高功率質子加速器和次臨界堆,該
靶件的物理作用主要是接受中、高能質子,發生散裂反應,產生中子源,引發持續鏈式反應,產生能量。
First line antituberculosis agents and major target mutations
抗結
核一線藥物及其主要作用
靶點的突變
In order to make the theoretical calculation feasible, we first obtain an analytical formalism of partial integrals with respect to the coordinates of the core and target in the phase - shift functions and their cross terms of scattering matrix elements, if the density distributions of the core and target are fitted to a few gaussian forms. then the rest multidimensional integrals with respect to the impact parameter and coordinates of halo nucleons are performed by a monte carlo method
為使理論計算變得可行,我們在
核芯和
靶核密度採用多個高斯分佈擬合的情況下,解析求解了各個散射矩陣元中的光學相移函數及交叉項含有的與暈
核核芯、
靶核密度分佈有關的積分;同時對與碰撞參數和暈
核子坐標有關的積分(八重以上,並且積分維數隨暈
核子數很快增加)採用蒙特卡洛方法計算。
After the estimation of labeling efficiency tp was applicated to hybridize the rna of tgev, then was subjected to self - development of x - ray exposed by cspd to show the target nucleic acid
對標記效率測定后,以帶正電的尼龍膜為介質進行特異性和靈敏性檢測,經非放射性自顯影來顯示
靶核酸。
Such a collective mode is not excited by a projectile with the same mass to change ratio as the target.
這種模式不能從與
靶核有相同荷質比的入射
核子激發出。
Based on theory of impact dynamics, the process of penetration test is simulated and the strength of supporting frame is checked
介紹了沖擊動力學的基本理論,對侵徹試驗中裝甲材料的破壞進行了數值模擬,校
核了試驗
靶架的強度,模擬結果和試驗值進行了比較。
These experimental results show that < q12 > is independent of the dimuon mass and logitudinal momentum, and imply the scattering of the incident quark within the target nucleus. by means of the glauber model, the difference of the mean transverse momentum squared < qt2 > is studied for the dependence of the incident proton energy and nucleus. the theoretical results are in good agreement with the fnal e772 experimental data
本文利用多重散射的glauber模型,討論了入射夸克在原子核中傳播的多重散射效應,給出了兩個不同的核drell - yan過程產生的末態輕子對橫動量平方平均值的差值對入射粒子能量核靶核的依賴性關系,計算結果與e772組的實驗數據符合甚好。