Considering of the specificity of the degenerative primer designed in this pcr reaction, the identity between the sequence we wanted and the fragment of pcr product and the presence of asmaspa, asmaspb, clr and cls ( the homologous gene of masp gene ) in halocynthia roretzi, a japanese ascidian, we believe that the sequence of pcr product is some part of the masp gene or masp homologous gene
基於本實驗中所設計的引物為特異性簡並引物，測序基因通過比較得到與預期片段有一定的同源性以及masp同源物asmaspa 、 asmaspb 、 clr和cls在海
鞘中存在的事實，我們可以初步推測，本實驗pcr反應所克隆的片段可能為文昌
魚masp或其同源基因的一部分序列。
When something took one of his remaining baits ， he cut the line with his sheath knife
當剩下的
魚餌中有一塊被咬住時，他就用自己那把帶
鞘的刀把釣絲給割斷了。
When something took one of his remaining baits, he cut the line with his sheath knife
當他剩下的魚餌中有一塊被咬住時，他就用自己那把帶鞘的刀把釣絲給割斷了。
