飼養細胞 的英文怎麼說

中文拼音 [sìyǎngxìbāo]
飼養細胞 英文
feeder cell
  • 飼 : Ⅰ動詞（飼養） raise; rear Ⅱ名詞（飼料） forage; fodder; feed
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 飼養 : raise; rear; feeding; raising
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. Es - d3 cells differentiated into ebs only when depleted of feeder cell layer by being cultured three generations in the presence of leukemia - inhibitory factor ( lif )

    結果表明： eso3細胞在含有lif的培養液中脫離飼養層培養3代以上才能形成ebs 。
  2. Eg cells of the 2th and 4th passage were akp ( alkaline phosphate ) positive. when cultured on degenerated feeder layers or in suspension, eg c ells formed embryoid bodies ( ebs ) in vitro

    當eg細胞脫離飼養層懸浮培養，或在衰老的飼養層上延遲培養時，發現eg細胞或單個存在，或聚集成團，形成類似於早期胚胎的囊狀胚體結構
  3. There is no different between the fibroblast feeder of mouse and the fibroblast feeder of goat according to blastoysts attached and icm proliferation. es - like cell develop embryoid body - like structure in suspension culture in vitro. es - like cells of goat differentiate into various kinds cell, egg : fibroblast cell, neurolike cell

    Es細胞在體外懸浮培養，形成類胚體，山羊類es細胞在無飼養層鋪有明膠的皿底上培養，可分化為多種類型細胞，如上皮細胞、成纖維細胞、神經細胞等。
  4. ( 3 ) isolation and culture of human primordial germ cells ( pgcs ). human pgcs collected from gonadal ridges and mesenteries were grown on mouse feeder layers in the presence of human recombinant leukemia inhibitory factor ( lif ), human recombinant basic fibroblast growth factor, and forskolin as described previously. initially, pgcs were visualized by alkaline phosphatase activity staining

    （ 3 ）人類pgcs的分離和培養從4 10周齡藥物流產胚胎的生殖嵴和腸系膜組織中分離原始生殖細胞（ primordialgermcells ， pgcs ） ，培養在添加人重組白血病抑制因子（ lif ） 、人重組堿性成纖維細胞生長因子（ bfgf ）和forskolin的小鼠飼養層細胞上。
  5. The quality of feeder layer is affected by a lot of factors, such as animal breed, culture medium, passages in vitro and experiment condition, etc. as to the production of feeder layer, there are a few reports about morphological and histologic change when of embryonic body fibroblast when culturing in vitro and cryopreservation, so kunming mouse were chosen as experimental animals and morphological and histologic changes were studied in course of its embryonic body culturing. we expect to offer theoretical foundation to our laboratory for setting up feeder layer storehouse. at the same time, the feasibility of myocardium tissue culturing with fibroblast layer altogether was studied so that established foundation for studied the biological characteristic of heart outside body

    小鼠胚體成纖維細胞的培養是制備飼養層的重要途徑，其制備、傳代及冷凍保存均有不同的研究報道，飼養層的質量受許多因素的影響，如動物的品種、培養液、所傳代數及實驗條件等，關于飼養層制備過程中的胚體細胞培養、傳代、冷凍后的細胞形態、組織學等方面的研究報道很少，故本實驗以昆明小白鼠為實驗動物，研究其胚體培養過程中細胞的形態學、組織學等方面的變化，以期為本實驗室建立飼養層細胞庫提供理論依據，同時探討心肌細胞和成纖維細胞層共培養的可行性，以期為心臟生物學特性的體外研究奠定基礎。
  6. The plaque - forming cell response to sheep red blood cells was found to be enhanced in mice fed a formula diet containing 20 g lactalbumin / 100 g diet in comparison to mice fed equivalent formula diets of similar nutritional efficiency containing 20 g / 100 g diet of either casein, soy, wheat or corn protein, egg albumin, beef or fish protein, spirulina maxima, or scenedesmus protein, or purina mouse chow

    摘要：本研究發現餵食特殊配方（ 20克乳白蛋白/ 100克飼料）小鼠的溶血斑形成細胞對綿羊紅血球的免疫反應增強，對照組餵食相等營養功效配方的酪蛋白、大豆、小麥或玉米蛋白、卵蛋白、牛肉或魚肉蛋白、以及其它三種市售蛋白飼料。
  7. The methods of evans and martin were changed slightly and used to isolate the mouse es cell in my experiment. in brief, the intact blastocysts were plated on sto feeder layer treated with mitomycin, and were cultured in the media supplymented with brl condition medium

    聯合evans和martin的方法，稍加改良來分離小鼠胚胎幹細胞，把昆明白小鼠完整的囊胚直接種植在經絲裂黴素滅活的sto飼養層細胞上，在含有brl條件培養基的es細胞培養液中培養。
  8. The results showed that in the beef cattle fed with chinese medicine additive, daily gain, number of lymphocyies and glucose and calcium concentrations in the blood were significantly increased, while npn content was decreased, some indexes, such as number of leucocyte, feed conversion rate and digestive rate of nutrients, had a tendency to rise, but there were no significant differences between treatment and control groups

    結果表明，肉牛日糧中添加中藥飼料添加劑，可顯著提高日增重、血液中淋巴細胞數量、血糖和血鈣含量，非蛋白氮含量顯著下降；血液中白細胞、分葉細胞、採食量、飼料轉化率、各養分消化率均有上升趨勢，但組間無顯著差異。
  9. Here we have used dye injection and gene gun labeling to identify and follow their differentiation in both normal and dark reared rabbits

    本篇研究主要是用微量注射和基因槍標定技術，觀察在全暗飼養下和正常光照飼養下兔子雙極細胞的分化情形有何不同。
  10. The primary primordial germ cells is obtain from the human embryos after 4 - 8 weeks of pregnancy. after mechanical desection and enzymic digestion, the cells were cultured on mouse embryonic flbroblast or human embryonic flbroblast feeder layer inactivated by mitomycin. the medium contains several cytokines : lif ( leukemia inhibitory factor ), bfgf and foskolin

    從4 ? 8周的流產胎兒的原始生殖嵴部位分離到原始生殖細胞，經過機械和化學方法的解離后培養於事先用絲裂黴素處理過的小鼠胚胎成纖維細胞或人胚胎成纖維細胞飼養層上，培養基中添加了lif ， bfgef ， foskolin等細胞因子，此後大約每7天左右傳代一次。
  11. The implantion percentage of embryos and the cloned es - like cells were low

    本實驗研究了胚齡、生長因子、飼養層對山羊es細胞分離和克隆的影響。
  12. At primary culture, pgcs co - cultured with their gonadal stromall cells were well grown. when subculture, we used primary chicken embryonic fibroblast ( pcef ), primary mice embryonic fibroblast ( pmef ) and snl cells to make feeder cells. forward research founded that the pcef cells were the most suitable for the growth of putative eg cells when having various cytokines

    繼代培養時，經過反復實驗比較雞胚原代成纖維細胞飼養層（ pcef ） 、小鼠原代成纖維細胞飼養層（ pmef ） 、 snl細胞飼養層等三種不同飼養層的作用效果，最終發現以雞胚原代成纖維細胞製作飼養層同時添加各種生長因子最適合雞類eg細胞的生長，但pcef與pmef之間的差異不顯著。
  13. By eye opening at p10 - 11, both normal and dark reared rabbits have possessed adult - like bipolar cells

    兔子出生后約10 - 11天開眼時，不論是正常環境飼養或是全暗環境飼養下的雙極細胞皆已經接近成熟的細胞了。
  14. With the increase of passage, the total of colonies decreased, but the number of classical nest - like or island - like eg cell colonies relatively increased. the growth of eg cells was dependent on the feeder layer

    傳代培養的eg細胞對飼養層有依賴性， icr品系小鼠pgc細胞和由其傳代后獲得的eg細胞呈明顯的群聚性，以單個存在狀態的較少
  15. We used the icr and kunming mice and got the embryos of 3. 5dpc by means of superovulation, then cultured the embryos on the feeder layer derived from icr or kunming mice, dispersed the inner cell masses ( icms ) or es cell colony in the appropriate time. in this period, we analized the effects of feeder layer, trypsin and embryos isolated from uteri of different varietal mice on the es cell lines

    以icr小鼠和昆明小鼠為實驗對象，採用超數排卵的方法獲得小鼠胚胎，培養在用絲裂黴素c處理過的icr小鼠和昆明小鼠mef細胞飼養層上，選取適當時間離散增殖的icm和類es細胞集落，分析了在小鼠es細胞建系過程中，飼養層細胞、消化液及不同品系來源的胚胎的影響。
  16. Mef were inactivated by treatment with 10ug / ml mitomycin c for 2. 5 - 3 hours or y irradiation 50gy / 75gy dose and grew in dmem as feeder layer to help mouse es cells and human pgcs

    採用10 g ml的絲裂黴素c或劑量為50gy和75gy的射線抑制mef有絲分裂，製作用於培養小鼠es細胞和人pgcs的飼養層。
  17. ( 5 ) when pgcs were cultured on different feeder layers, the better results were got if pgcs were cultured on sto and mef1 feeder layers, when pgcs were cultured on thawed sto feeders, pef, inactivated pef feeders, 0. 1 % gelatin, we can not get typical eg colonies

    （ 5 ）用不同飼養層培養pgc ，實驗結果證明pgc在新鮮製作的sto和mef ~ 1飼養層上，可以得到傳3代以上的eg集落； pgc在鋪有解凍的sto飼養層細胞、 pef或絲裂黴素c處理過的pef 、塗有0 . 1明膠的4孔板上，一直沒有得到典型的eg集落。
  18. After 4 days of growth, the intact inner cell mass ( icm ) were separated with 0. 05 % trypsin - edta and replated on feeder layer in dmem containing 15 % serum, 0. 1mmol / l nonessential amino acids, 0. 1mol / l 2 - mercaptoethanol, 2mmol / l glutamine, 100 units / ml of streptomycin and 100 units / ml of penicillin. after 4 - 6 days 5 es cell colonies were selected and expanded in which alkaline alkaline phosphatase was detected

    （ 2 ）小鼠囊胚或內細胞的培養和es細胞的分離培養了156個不同品系的小鼠囊胚，經過3 ? 4天培養后，將增殖出來內細胞團用機械法結合胰酶- edta處理，離散后培養于mef飼養層上， 4 6天後有5例出現了es細胞樣集落。
  19. L. a total of 312 fetuses were collected from 27 wuzhishan inbreeding pigs ( wzsp ) and white minitype pigs to study the factors influencing the establishment of porcine embryonic germ cells, and we draw the following conclusions : ( 1 ) we can get embryonic fibroblasts from d26 - 28 fetuses ( day of first mating is day 0, that is d 0 ) ; in this thesis, sto and mef1 were inactivated by incubation in medium containing 10 g / ml of mitomycin c for 1. 75h ~ 2h ; while pef were inactivated by the same medium for 2. 25h ~ 2. 5h

    本實驗以中國近交系五指山小型豬（ wzsp ）和微型白豬為材料，先後從27頭豬採集了312枚早期胎兒用於進行豬胚胎幹細胞（ eg ）建系影響因素研究，得出如下結論： （ 1 ） d26 - 28豬胎兒可用於製作胎兒成纖維細胞。製作飼養層時， sto 、 mef ~ 1細胞用10 g ml絲裂酶素c處理1 . 75h 2h ； pef細胞用絲裂酶素c處理2 . 25h 2 . 5h 。
  20. Effects of mouse embryonic fibroblast feeder layers and combined of mouse embryonic fibroblast and collagen of rat tail on adhesion of cultured cells from schistosoma japonicum

    鼠胚胎成纖維細胞飼養層及與鼠尾膠聯合促日本血吸蟲培養細胞貼壁作用的研究
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