養蠶家 的英文怎麼說
中文拼音 [yǎngcánjiā]
養蠶家
英文
sericiculturist/sericulturist-
China was the first country in the world to cultivate silkworms and develop silk weaving. more than 3, 000 years ago, sericulture and silk weaving were already significantly developed.
中國是世界上最早發明養蠶和絲織的國家;早在3000多年以前,蠶桑、絲織已相當發達。During the different time point, we retrieve the cell supernatant and through the electrophoretic analysis and immunoblot of the recombinant protein indicated that the cell which transfected the recombinant eukaryotic gene expressing vector could synthesis and excrete the peptide effectively
提取培養細胞總rna反轉錄cdna測序結果表明人生長激素基因組dna在家蠶bmn細胞內能正確轉錄,剪接。蛋白質電泳分析和免疫學檢測證明轉染細胞能夠有效合成並分泌hgh蛋白質。The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses
同時,還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試,並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學,細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞,但其遺傳學和細胞生物學背景知之甚少。Techniques of culture of prothoracic gland ( pg ) in vitro, 3h - ecdysteroid - radiounniunoassay, and methods of molecular biology and biophysics, for instance, nuclear magnetic resonance ( nmr ), circular dichroism cd ) and so on were engaged. the biological activity on ecdysteroidogenesis in vitro and in vivo of a group of analog peptides of bom - ptsp synthesized by chemical modifications and the relationship between the structures and functions were studied
本研究利用~ 3h -蛻皮激素放射免疫分析方法、分子生物學、生物物理學的方法和手段、體外培養和體內導入等技術對bom - ptsp及其一組人工合成的與ptsp結構類似的多肽進行了結構和功能關系的研究,鑒定了它們的生理活性及其與結構的關系,以及它們在家蠶體內的活性及其與結構的關系,還對ptsp及其類似多肽進行了核磁共振和圓二色的研究。Based on archaeological findings, experts made estimates that ever since mid - nellithic age which is five or six thousand years ago, china has engaged in raising silkworms, obtaining silk and weaving silk fabric
專家們根據考古學的發現推測,在距今五六千年前的新石器時期中期,中國便開始了養蠶、取絲、織綢了。No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract
將重組病毒rbmbacph - egf以10moi感染bmn細胞, 72小時后收集培養細胞和上清;培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物,粗略估計表達量約6 7 g 2 10 ~ 6個細胞(相當于egf標準品) ;重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲,每隔24h收集蠶血淋巴,經elisa檢測發現第4天表達量最高,根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ; elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。By this method of primary culture, cells and tissues could not only subsist for 11 months and more in vitro, but also be subcultured. these works lay a foundation of the establishment of silkworm embryonic cell line
為建立新的家蠶胚胎細胞系奠定了一定的基礎。原代培養的細胞和組織塊在體外有一定的生長現象,細胞體外最長存活達門個月以上。The main function of bom - ptsp is that it inhibits ecdysteroidogenesis when prothoracic gland ( pg ) of the silkworm was active, and when pg is not active, prothoracicotropic hormone ( ptth ) activate pg, whereas bom - ptsp almost completely inhibits ptth - stimulated ecdysteroidogenesis when pg was incubated in medium of ptth and bom - ptsp combination in vitro. ptsp cooperates with ptth to regulate and control ecdysteroidogenesis on prothoracic gland of insect
它的主要作用是:當家蠶前胸腺( prothoracicgland , pg )處于活動時期,該多肽能強列地抑制前胸腺合成蛻皮激素;當前胸腺處于非活動時期,促前胸腺素( prothoracicotropichormone , ptth )可以激活前胸腺;同時用ptth和ptsp體外培養前胸腺時,被ptth激活的蛻皮激素的生物合成又可被ptsp所抑制。The enzymatic activity of the both prokaryotic and eukaryotic derived appa phytase were increased in the presence of ca2 + and mn2 + at a concentration of 1 mm. due to the high expression efficiency and the enzymatic characteristics, bevs derived appa phytase seemed to be a valuable candidate for the application of feed supplement
由appa植酸酶在家蠶生物反應器中的超量表達以及appa植酸酶本身所具有的酶學特性推斷,家蠶表達的appa植酸酶適合於作為新一代植酸酶添加劑應用於飼料工業與畜禽養殖業中。This dissertaion, on the basis of the other studies, on the one hand, researched and analysed the chromosomes and mitoses of the cell line - bmn, which is widely used in the silkworm baculovirus expression system as an engineering cell. on the other hand, the dissertation attempted to explore establishment of the new silkworm embryoni cell line by primary culture
同時,家蠶體外細胞培養研究的進行,不僅可以為家蠶細胞生物學的基礎理論研究提供良好的研究系統,而且在家蠶資源的開發與利用方面也具有重大的意義。本研究在借鑒前人研究的基礎上,一方面對現在廣泛應用於家蠶桿狀病毒表達系統的工程細胞? bmn細胞的染色體和有絲分裂進行了研究與分析。2. the primary culture of silkworm embryonic cells ( 1 ) this dissertation took silkworm blastokinesis embryo as the materials of primary culture and found a better method of fragment embryo to primary culture by comparing the methods of fragment embryo that mincing embryo by scissors and digest embryo by trypsin after mincinging. that is the latter
2家蠶胚胎細胞的原代培養門)本研究以家蠶反轉期胚胎為材料,採用組織塊法和胰蛋白酶半消化法進行家蠶胚胎細胞的原代培養,建立了適應反轉期胚胎細胞原代培養的方法,即胰蛋白酶半消化法。The bmn cells and fifth instars were infected by the recombinant virus bacpak - angiostatin. the bioactivity of the protein product was determined by human umbilical vein endothelial cells ( ecv304 ) growth assay in vitro and inhibition of vascular growth assay in cam in vivo. angiostatin showed significantly inhibitory effect on endothelial cells
重組病毒以moi = 10感染家蠶細胞( 2 10 ~ 6個細胞/ 5ml )和家蠶5齡幼蟲,表達產物用體外培養的人臍靜脈血管內皮細胞( ecv304 )及體內雞胚尿囊膜( cam )新生血管實驗檢測其抑制活性: (一)血管抑素可明顯抑制體外培養的內皮細胞增殖。分享友人