高酶菌株 的英文怎麼說

中文拼音 [gāojūnzhū]
高酶菌株 英文
hyper strain
  • 高 : Ⅰ形容詞1 （從下向上距離大; 離地面遠） tall; high 2 （在一般標準或平均程度之上; 等級在上的） above...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 株 : Ⅰ名詞1. （露在地面上樹木的根和莖） root and stem of a tree above the ground 2. （植株） individual plant; plant Ⅱ量詞（棵）
  • 菌株 : bacterial strain
  1. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  2. Studies on screening of strains of producing phytase and the conditions of producing phytase the strains of producing phytase could be identified by the hydrolysis bound in differential medium. aspergillus niger an010001 secreting phytase was isolated by screening and second screening. the conditions of producing phytase was studied

    植酸酶菌株的篩選及產酶條件的研究本項研究利用植酸酶的菌株能在篩選培養基上形成水解透明圈的特點而進行鑒定，通過初篩和復篩，得到一株產植酸酶較高的黑麴黴（ aspergillusniger ） an00101菌株。
  3. In this paper, a strain was selected with high esterase activity. its characteristics of production and enzymology and separation of l - lactic acid were investigated. through primary screening and rescreening, a fungus, f - 16, with high esterase activity was selected

    本文選育出了高活性酯酶產生菌株並對該菌體的產酶性質、酶學特性及酶對乳酸乙酯的手性拆分后產物分離作了一定的研究。
  4. It has been 40 years since dobereiner and ruschel isolated the nitrogen - fixing bacteria from the rhizosphere of sugarcance plants and demonstrated the potential of diazotrophs to associate with graminaceous plants. more recent evidence of significant biological nitrogen fixation in economical important graminous species, particularly sugar cane, rice and forage grasses, has induced tremendous interest in ni fixation by non - legumes

    本研究分離、篩選得到一株固氮酶活性高且穩定，生長勢強的聯合固氮菌株，並對其分類地位、形態及生理特徵、對環境的適應性及其對植物的促生效果和作用機理作了系統研究，得結果如下。
  5. In this paper, thirty strains of yeast were isolated from nineteen samples of fermented milk ( ten koumiss samples, three fermented goat milk samples, four fermented milk samples, two shubat samples ) which were collected from the west - centre region of inner mongolia from which we aimed to select the yeast with high vitalistic ? galactosidase. according to the results of dedermination that were the metabolism rate and ratio vitality of ? galactosidase, stk - 1 - 1 has been selected. the result of identification showed that stk - 1 - 1 belonged to kluyveromyces marxianus of kluyveromyces

    本研究主要以內蒙古中西部地區牧區採集的19個發酵乳樣品（ 10份酸馬奶、 3份發酵山羊奶、 4份酸牛奶和2份發酵駝奶）為研究對象，分離獲得30株酵母菌，從中篩選乳糖酶高活力酵母菌株。根據乳糖代謝率和乳糖酶比活力的測定結果，選出菌株stk - 1 - 1為優勝菌株。經鑒定，該菌株屬克魯維酵母屬（ kluyveromyces ）的馬克斯克魯維酵母（ kluyveromycesmarxianus ） 。
  6. Breeding of alkaline lipase overproducing strain by screening resistant mutant

    抗性突變株篩選法選育堿性脂肪酶高產菌
  7. Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus

    本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。
  8. The page revealed the culture supernatant of the initial strain and the mutant contained different protein bands, which exactly demonstrated at protein level that a. niger j 506 was surely a mutant of a. niger m1. zymogram stained with xylan - remazol brilliant blue for detecting xylanase showed there are three different xylanases in the mutant culture, while two xylanases in initial strain. what is important, the third xylanase in a. niger j 506 have higher activity and more production levels from page and zymogram of xylanase

    尤其是在木聚糖酶譜帶檢測中發現，突變株發酵液中有三種類型的木聚糖酶，而出發菌株中只有兩種類型的木聚糖酶，並且通過考馬斯亮藍g250染色和瓊脂糖板上的透明圈發現，突變株中第三種類型的木聚糖酶不僅表達量很大，活力也很高。
  9. Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "

    W12菌株對環境因子的適應性研究：無氮培養條件下，測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響，結果表明，在15 - 40下均能生長並表達固氮酶活性，其最適生長及固氮的溫度為25 - 35 ；能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮，當培養基中同時存在蔗糖和蘋果酸時，細菌生長和固氮活性最強；在偏酸和偏堿的條件下（ ph4 . 5 - 8 . 5 ）均能保持較強的生長勢和較高的固氮酶活性，並能通過調節自身代謝平衡並適應環境的酸、堿性變化，使培養液趨于中性：能耐受較高的滲透壓，培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大，當naci濃度升至2時，菌株的生長勢及固氮酶活性才有所下降：低濃度的鉸對其固氮酶活性影響不大，在0
  10. Conclusion a systematic method for preparation of enzyme - mannanse is established, a high productive strain was got after seducing and selecting from nature, confirmed as brachybacterium spa6 research were conducted on medium and culture method of the strain in order to get the suitable cultural condition of fermentation, the experiment result shows the optimium condition is ph7. 0, temperature 36c ; carbon content 2. 5 %, ventilation in abundence, agitation speed 200r / min

    結論1 、以從自然界中篩選出的菌株為出發株，經誘變、篩選，得一高產葡甘聚糖酶菌株，初步鑒定為短桿菌屬brachybacteriumspa6 2 、經誘變、三角瓶培養，該菌株的最適培養條件：培養基ph值7 . 0 ，碳源2 . 5 ，振蕩培養， 200r min ，培養溫度36 ，培養48h 。
  11. Through screening a lot of mutants with the method of transparent zones and culture filtrate, the best four were obtained with high - yield of stable phb depolymerase, named as 02, 04, 09 and 14

    以青黴（ penicillium . sp ） ds9701為出發菌株，通過紫外線誘變分生孢子，採用透明圈初篩和搖瓶培養復篩的方法，獲得4個能穩定遺傳的phb解聚酶高產菌株。
  12. Hensing m. production of extracellular inulinase in high - cell - density fed - batch cultures of kluyveromyces marxianus [ j ]. appl microbiol biotechnol, 1994, 42 : 516 - 521

    王建華，姚斌，王亞茹.一株產菊粉酶的酵母菌株及其在製作高果糖漿中的應用[ p ] .中國專利: 1252438a . 2000 - 5 - 10
  13. The supernatant fraction and the precipitation fractions were analyzed by western blotfor strain dh5 a / pkkfpga, 5 - 10 % pga precursors formed as inclusion bodies in the cytoplasm while no inclusion bodies formed in the periplasm, this suggested most pga precursors were transported to the periplasm and matured to active pga and indicated that the maturation of pga in strain dh5 / pkkfpga was limited by the translocation step

    Western印跡分析表明對于菌株dh5 pkkfpga ， 5 - 10的原前體青黴g素酰化酶在胞內形成了包涵體，說明其成熟的限速步驟在胞內的運輸階段，而菌株dh5 psmlfpga則無明顯包涵體形成，說明菌株dh5 psmlfpga改善了青霉素g酰化酶的合成流，因而其表達能力高於菌株dh5 pkkfpga 。
  14. Study on screening a high - productive strain of dextranase and its basic conditions of flask fermentation

    葡聚糖酶高產菌株的誘變篩選及其搖瓶發酵條件的初步研究
  15. Effect of medium components on enzyme production and characterization of anaerobic fungal crude enzymes were also investigated. this thesis was described in the following three sections. in the first section, twelve anaerobic fungal strains isolated from rumen and faeces of ruminants were screened for xylanase and cmcase production

    本研究從黑白花種公牛、水牛、山羊糞樣及山羊瘤胃內容物中分離到12株厭氧真菌，並對其進行了產高活性羧甲基纖維素酶和木聚糖酶菌株的篩選，同時還就培養基主要組分對厭氧真菌產酶的影響和厭氧真菌的粗酶性質進行了研究。
  16. Over 521 strains were screened for phytase production from 30 kinds of soil samples. out of them, four strains were obtained by the primary screening, then, one strain ( lw03 ), which has higher levels phytase production, was screened by enzyme yielding tests. lw03 strain was primarily identified to be rhizopus

    以lw03為出發菌株經紫外線、 des 、 co ~ （ 60 ）射線和ntg等連續誘變及篩選，得到1株植酸酶高產菌株no13 ，並對根霉no13菌株固態產酶條件、部分酶學性質及其在米糠、菜籽粕植酸體外降解試驗進行了研究。
  17. The pcr products of gyra of a resistant strain ( sll - 3 ) was sequenced and analysed. when compared to the corresponding sequence of the gyra of salmonella typhimurium from the nucleotide sequences data of the gyra reported by griggs, 5 mutants sites were found in the qrdr. dma sequencing identified in the strain sll - 3 ser to phe change at codon 83 of gyra gene

    對所試菌株染色體的pcr產物行hinfi酶切后電泳檢測表明， cip高敏菌株（ 6株）得到預期大小為363bp 、 206bp 、 99bp的3個條帶；而耐藥菌株和中敏菌株（ 10株）電泳檢測到大小為363bp和305bp的2個條帶。
  18. In total 102 strains of acidic phytase producing strains were selected from soil by selective plate containing calcium phytate. among them 32 strains with relatively large clear circle were purified and re - selected by shaking - culturing. after fermented for 5days at 28 c and shaking at 220r / min, the activity of phytase was determined by nh4vo4 - ( nh4 ) 6mo7o24 method at 37 c and ph2. 5 or ph5. 5

    主要結果如下： 1植酸酶高產菌株的篩選利用植酸鈣選擇性子板培養基從土樣中篩選出102株酸性植酸酶產生菌，從中挑選出透明圈較大的菌株32株，經分離純化後分別進行搖瓶復篩， 28 、 220r / min發酵5天後，在37 、 ph2 . 5或ph5 . 5條件下用釩鉬酸銨法檢測其酶活，結果發現有3株菌產酶活性較高且產酶性能較為穩定。
  19. The folin method was used to measure the activity of proteases and find out the most active strain

    用福林酚法測定其蛋白酶活力，並確定活力最高的菌株。
  20. This investigation aims to screen out the high - yield acidic phytase - producing microbial strains and men further to select for high yield strains by mutagenesis and still then to clone and analyze the phytase gene in the high - yield phytase - producing mutant strains. on these bases, to construct high yield phytase gene engineered microbial strains for the industrialized production of phytase. main results are as follows

    本研究的目的旨在通過從土壤中篩選酸性植酸酶高產菌株，並進一步通過誘變選育提高其產量，對高產突變菌株植酸酶基因進行克隆和分析，在此基礎上，構建植酸酶高產基因工程菌，從而為植酸酶產品的工業化生產奠定基礎。
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