點突變 的英文怎麼說
中文拼音 [diǎntūbiàn]
點突變
英文
point mutation-
Detection of fibroblast growth factor receptor 3 gene mutation at nucleotide 1138 site in congenital achondroplasia patients
先天性軟骨發育不全成纖維細胞生長因子受體3基因1138位核苷酸點突變的檢測The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation
利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。A cusp catastrophe model of unstable failure process of quasi - brittle materials
準脆性材料破裂過程失穩的尖點突變模型Influence of 3 ' untranslated region single nucleotide polymorphism of tlr4 gene on luciferase gene expression
位點突變對熒光素酶表達的影響Site - specific mutagenesis of murine anti - human tnf - fab gene and its expression in e. coli
基因的定點突變及其在大腸桿菌中的表達Pcr method was used to identify candidate ms 188. sequence analysis indicated that a point mutation occurred in the second exon of the atmyb103 gene in male sterile mutant with caa ( gln ) replaced by a stop codon taa
利用pcr的方法從突變體中擴增atmyb103基因並進行序列分析，結果表明突變體中atmyb103基因第二個外顯子上發生了點突變，由原來編碼谷氨酰胺的caa密碼子突變為終止密碼子taa 。In escherichia coli, arog gene encodes phenylalanine - sensitive 3 - deoxy - d - arabino - heptulosonate - 7 - phosphate synthase isoenzyme arog that catalyzes the first committed step of shikimate pathway. here we study the essential amino acid residues involved in the formation of feedback inhibition site of arog, and the effects of n - terminus on feedback inhibition and its quaternary structure, and the importance of the structural " d2 " symmetry to allosteric inhibition
本博士論文工作以大腸桿菌k - 12來源的arog為研究對象，通過定點突變、反饋抑制實驗和酶學動力學參數的測定，深入地研究了arog的反饋抑制位點的特性，並對arog的n -末端在反饋抑制機理和維持穩定四級結構中的作用，以及蛋白質結構的「 d2 」對稱性對酶功能的重要性等進行了具體的研究。Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various hydroxynitrile lyases
研究人員已經利用蛋白質工程和定點突變技術來改變各種醇腈酶的活性位點和底物特異性等。The principles, operation and applications of site - directed mutagenesis, gene fusion technology, and post - translational modification methods were introduced emphatically
著重闡述了基因定點突變技術、基因融合技術和翻譯修飾技術等新興定點固定化技術的原理、特點和操作。Okamoto h, tsuda f, akohane y, et al. hepatitis b virus with mutation in the e antigen - negative phenotype in carriers with e antibody to e antigen [ j ]. j virol, 1994, 68 : 8102
王小飛，劉華瑞，王錦蓉，等.乙型肝炎和肝癌患者乙型肝炎病毒前c區1896位點突變的研究[ j ] .中華傳染病雜志， 1996 ， 14 : 11We successfully construct the eukaryotic expression vector of gfp - eif - 5a and its mutational vector using genetic engineering techniques. we found that eef - 5 a localized in nucleus as well as in cytoplasm just for a short time after its transient expression, then distributed only in cytoplasm
Eif - 5a的hypusine修飾是其活性和功能發揮所必需的，我們通過pcr方法實現了hypusine位點的定點突變，並進一步構建了含gfp標簽的eif - 5a及其hypusine位點突變的真核表達載體。Phylogenetic tree indicates the rab protein may play a role in vesicular trafficking from endoplasmic reticulum to golgi body. two tga in eo - rabl were successfully mutated to tgc by site direct pcr procedure and the truncate eo - rabl was obtained
以此重組質粒為模板進行pcr定點突變，將eo - rab1基因中前兩個tga突變為通用半胱氨酸密碼子tgc ，獲得截短型eo - rab1基因。With strong specificity and easy to manipulation, this simple, inexpensive, rapid molecular beacon hybridization technique permits visual monitoring of gene point mutation and snp, which shows better advantage than pcr - sscp. 2
Mb雜交技術特異性強，簡單快捷，能進行可視性檢測，完全適用於點突變和snp的研究，技術特點明顯優于pcr sscp 。It was interested that there was an extra six nucleosides insertion between 1647 - 1652nt ( according to the genomic sequence of la sota strain ), and the sequence were cccccc in f48e9 strain, and tcccac in zj1 strain. in order to test if insertion of this six nucleosides is related with the virulence of nd, two primers were designed to amplify the same fragment of another ten ndv strains. the result of sequence comparison of 16 strains showed that the six nucleoside was absent in lentogenic strain. this suggested that the six nucleosides insertion might have relationship with the ndv virulence. compared with all known sequence of ndv. there was a special sequence ( 5 ' tctctctcctctctcctcc3 " ) in the genomic cdna of ndv f48e9 strain
通過rt - pcr方法擴增獲得了另外10個背景清楚毒株的np - p基因間隔區片段，將這些序列與f48e9 、 lasota 、 clone30 、 b1 、 zj1和v4的相應序列進行了比較，結果在參比的16個ndv毒株中在該區段中除了有多個點突變外，個別毒株有堿基插入和缺失，所有以lasota株為代表的弱毒株均無6堿基的插入，而以f48e9株為首的強毒株均有此6堿基的插入，但有一個中等毒力的毒株dp沒有6堿基的插入，不過它的基因序列和lasota的幾乎相同，對于所克隆到的基因的代表性還有待確定。The high - enzyme activity has 2 base changes, resulting in long amino acid sequence with native amylase. this inducing method resolved the problem of non - effective induction as in base analogue induction. and the method we used provide a new measure for this kind of work
高酶活編碼區位點突變導致c -端序列變化和終止子的后移本誘變方法克服了用堿基類似物在體內誘變由於核酸復制酶等的校正作用而造成誘變無效的難題，為基因的誘變找到了一條新途經。Mms, a kind of dna - damaging agent, predominantly methylates ring nitrogens of pyrine, leading to dna double - strand breaks, point mutation and so on
甲磺酸甲酯（ methylmetbanesulfonate ， mms ）是一種能夠損傷dna的烷化劑，主要使dna嘌呤堿基上的o 、 n甲基化，造成dna雙鏈斷裂、點突變。Sequencing result showed that we had gotten the single mutants
測序結果表明得到了點突變。Earthquake - induced liquefaction and pore water pressure based on cusp catastrophe model
基於尖點突變模型的地震液化和孔壓研究A cusp catastrophe model of the sticky expectation of the speculator in the futures markets
期貨市場投機者粘性預期的尖點突變模型We did not find the c57 1t c628t. c658t or a375g point mutations in both populations
在兩個人群中均未檢測到攜帶c571t 、 c628t 、 c658t和a375g點突變的個體。分享友人