affinity of antibody 中文意思是什麼
affinity of antibody
解釋
抗體親和力-
After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum
使用分步透析法對變性的包涵體進行復性，將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原，初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。 -
The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。 -
Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use
X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。 -
Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。 -
Presl ( 20 - 47 ) is one of the important epitope of hbsag, which has been pointed out to have relationship with infection of hbv. humanized antibody against presl with high affinity is a new way to block hbv
乙肝病毒表面抗原pres1 （ 20 - 47 ）表位是重要的乙肝病毒（ hbv ）表位，其識別肝細胞受體，並與hbv侵染人肝細胞有關。 -
The characteristics of quantum computing and the mechanism of immune evolution are analyzed and discussed. inspired by the mechanism in which immune cell can gradually accomplish affinity maturation during the self - evolution process, a immune evolutionary algorithm based on quantum computing ( mqea ) is proposed. the algorithm can find out optimal solution by the mechanism in which antibody can be clone selected, memory cells can be produced, similar antibodies can be suppressed and immune cell can be expressed as quantum bit ( q - bit ). it not only can maintain quite nicely the population diversity than the classical evolutionary algorithm, but also can help to accelerate the convergence speed and converge to the global optimal solution rapidly. the convergence of the mqea is proved and its superiority is shown by some simulation experiments in this paper
分析和探討了量子計算的特點及免疫進化機制，並結合免疫系統的動力學模型和免疫細胞在自我進化中的親和度成熟機理，提出了一種基於量子計算的免疫進化演算法.該演算法使用量子比特表達染色體，通過免疫克隆、記憶細胞產生和抗體相似性抑制等進化機制可最終找出最優解，它比傳統的量子進化演算法具有更好的種群多樣性、更快的收斂速度和全局尋優能力.在此不僅從理論上證明了該演算法的收斂，而且通過模擬實驗表明了該演算法的優越性 -
Abstract : the characteristics of quantum computing and the mechanism of immune evolution are analyzed and discussed. inspired by the mechanism in which immune cell can gradually accomplish affinity maturation during the self - evolution process, a immune evolutionary algorithm based on quantum computing ( mqea ) is proposed. the algorithm can find out optimal solution by the mechanism in which antibody can be clone selected, memory cells can be produced, similar antibodies can be suppressed and immune cell can be expressed as quantum bit ( q - bit ). it not only can maintain quite nicely the population diversity than the classical evolutionary algorithm, but also can help to accelerate the convergence speed and converge to the global optimal solution rapidly. the convergence of the mqea is proved and its superiority is shown by some simulation experiments in this paper
文摘:分析和探討了量子計算的特點及免疫進化機制，並結合免疫系統的動力學模型和免疫細胞在自我進化中的親和度成熟機理，提出了一種基於量子計算的免疫進化演算法.該演算法使用量子比特表達染色體，通過免疫克隆、記憶細胞產生和抗體相似性抑制等進化機制可最終找出最優解，它比傳統的量子進化演算法具有更好的種群多樣性、更快的收斂速度和全局尋優能力.在此不僅從理論上證明了該演算法的收斂，而且通過模擬實驗表明了該演算法的優越性 -
According to antibody - antibody ab - ab affinity and antibody - antigen ab - ag affinity, the algorithm can allot adaptively the scales of memory unit and antibody population. it is proved theoretically that the csaim is convergent with probability 1
由於遺傳和免疫細胞在增殖中的基因突變，形成了免疫細胞的多樣性，這些細胞的不斷增殖形成無性繁殖系，無性繁殖稱為克隆。 -
Indeed, through progress and increased experience in library design and selection technologies, gained not least from working with synthetic antibody libraries, researchers have now exploited many of these novel scaffolds as tailor - made affinity reagents
事實上，隨著庫的設計和篩選技術的不斷進步及經驗的積累，研究人員不僅僅是從人工抗體庫中，還開發了很多新的平臺來進行高親和力特定結合分子的設計。 -
After the lysate was purified with gst sepharose 4b affinity chromatography, a specific band of 34kd appeared in sds - page gel, the purity and the titer of the antibody against fusion protein gst - hnadc3 could reach up to 90 % and 1 : 32, respectively
菌體裂解液經gst親和層析純化后， sds page上出現一分子量為34kd的特異蛋白條帶，純度可達90 。 -
In the first part, we observed the changes of expressions of type i receptor of il - 1 in the rat and mouse brain after intraperitoneally administration of different kinds and doses antigens respectively. in the second part including two experiments we cloned rat il - 6r ' s genes by pcr, expressed them in e. coli dh5 a and cos - 7 cell, and produced il - 6r ' s polyclonal antibody which is proved having more high liter, affinity and specificity 1
第一部分採用不同種類和劑量的抗原刺激，分兩個實驗，觀察了大、小鼠腦內il - 1的i型受體表達的變化，探討了腦內參與免疫調控的核團和細胞類型；第二部分分兩個實驗，運用pcr技術克隆了大鼠il - 6r的基因並進行了原核和真核表達，制備了特異性高、親和力強和較高效價的抗il - 6r多克隆抗體，為進一步進行il - 6r的研究奠定了基礎。 -
The specificity and affinity and antibody are the key points of antibody based immunotherapy for target andtibody binding and for expected effects
為此，我們首先制備、純化了免抗eev病毒抗體及小鼠抗人cd55的抗體。 -
Independent clones was constructed and affinity panning of anti - n - peptide antibody fragments was carried out
的小鼠噬菌體抗體庫，並從中淘選出對n -肽有結合活性的單鏈抗體。
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