agarose 中文意思是什麼
agarose
解釋
瓊膠糖-
These liabilities prompted development of agarose and agarose-acrylamide gels.
這些不穩定性促使瓊脂糖和瓊脂糖--丙烯酰胺凝膠的發展。 -
Agarose is naturally linear polysaccharide.
瓊脂糖是一種天然的直鏈多糖。 -
Used the genomic dna extracted by low melting - point agarose embedding method as pcr template, the full length of structural genes of bacillus subtilis bio operon were gained by long pcr method
將該方法提取的基因組dna稀釋100倍作為模板，採用長距離pcr方法，獲得了枯草桿菌生物素操縱子基因全長。 -
In order to discuss the application of sol - gel in the preparation of biomembrane, the gel membrane of agarose was prepared by sol - gel with acticarbon and agarose as raw material, at the same time, the catalytic activity of immobilized cod based in the membrane was studied
為探討生物傳感器用膜的制備，採用溶膠-凝膠法，以活性炭微粉和瓊脂糖制備了瓊脂糖凝膠薄膜並研究了薄膜固定cod的催化特性。 -
The 2nd pair of primers was designed according to the sequence of strain th - 98 collected in genbank. concentration of tp was analyzed after amplification invitro by rt - pcr, purified by low melt agarose and labeled by digoxigenin
根據genbankth - 98株序列設計第2對引物，應用rt - pcr方法體外擴增該片段（命名為tp ） ，瓊脂糖凝膠純化后測定其濃度和純度，進行非放射性地高辛標記。 -
In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability, in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry, preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization
本研究用改良后的親和層析法分離純化mr ， lowry法測定其蛋白質濃度，在精子穿透試驗（ spermpenetrationassay ， spa ）模型中定量研究其對精卵融合能力的影響並檢測其細胞生物學活性；以已知濃度的pmr （ purifiedmannose - ligandreceptor ）干預精子半透明帶試驗，觀察用pmr預處理半透明帶對精子與透明帶結合的影響。 -
The result of the agarose gel electrophoresis showed that the length of the full - length cdnas in the library was pooled mainly between 500 and 2 000 base pairs
結果表明獲得的ejoi基因的cdna長度為876hp ，開放閱讀框長度為759hp ，編碼252個氨基酸。 -
( 5 ) conventional, two dimension ( agarose coating ) and three dimension ( agarose embed ) culture system were compared in their efficiency of supporting follicle growth
（ 5 ）比較了普通培養法、二維培養法（瓊脂糖鋪底）和三維培養法（瓊脂糖包埋）的水牛腔前卵泡培養效果。 -
The results also explained that it was feasible that the genomic dna of bacillus subtilis were extracted and gained large dna fragments by low melting - point agarose embedding method
說明以低熔點瓊脂糖包埋法提取枯草桿菌基因組dna ，並獲得較大的dna片段，是完全可行的。 -
Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods
大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。 -
The pcr products were electrophoresed on 3 % agarose gel and visualized under uv light
聚合酶鏈鎖反應的產物以3 %瓊脂電泳分離並在紫外光下觀察結果。 -
Materials and methods in our study, first the e. coli bl21 transformed already are cultivated in smaller scale and then the plasmids dna were extracted by the methods of alkaline lysis. the plasmids dna extraction were processed 37 overnight by the restrictive endo - incisase bam h i and xho i. the incision products were used to check the integrality and variability of the recombinant plasmids dna by 1 % agarose gel elec - trophoresis
同預想結果基本吻合，大規模培養后純化得到的融合蛋白通過sds page顯示在52kda處有一條帶，而酶切后產物電泳結果顯示在26kda處分另有兩條蛋白帶，其中一條為gst ，另一條為hbrp ，基本符合實驗結果； hbrp對tpk活性的抑制呈劑量依賴性。 -
Standard practice for detection of mycoplasma contamination of cell cultures by growth on agarose medium
用瓊脂生長法直接檢測細胞培養中枝原體屬染菌的標準實施規程 -
Apoptotic peak. agarose gel electrophoresis showed that 185 - base pair ladder characteristic of the dna degradation that occurs in apoptotic cells induced by a
電泳出現階梯狀條帶流式細胞儀檢測出現典型的凋亡峰，提示 -
Thermocycled pcr samples were resolved electrophoretically on 1. 5 % agarose gels and taken photos using a polaroid camera. the statistical results were analyzed by the spss software, and the cluster figures were obtained. conclusions could be drawn from the study as following : 1 ) the molecular systematics of 57 species of crickets, which belong to 26 genera 7 family in grylloidea, had been studied by the approach of rapd
本項研究在依據外部形態分類鑒定及前人工作的基礎上，採用rapd技術，通過對蟋蟀總科7科26屬57種蟋蟀基因組dna的rapd圖譜的比較研究，在分子水平上探討這些類群的分類地位和親緣關系，為豐富蟋蟀總科的分子系統學研究，並為進一步完善蟋蟀總科的分類系統，揭示其系統發育及演化提供分子水平的依據。 -
The apoptosis induced by extract of russula subnigricans hongo was investigated in little white rat liver and kidney cells by agarose gel electrophoresis. the result showed that agarose gel electrophoresis of dna extracted from poisoned little white rat liver and kidney cells revealed typical 180 ~ 200bp integer - fold " ladder " " bands. apoptosis induced by extract of russula subnigricans hongo was dose - and time - dependentthe result indicated that extract of russula subnigricans hongo could induce apoptosis in little white rat liver and kidney cells
4 .用電泳技術研究亞稀褶黑菇粗毒液誘導小白鼠肝腎細胞凋亡，小白鼠亞稀褶黑菇抽提液中毒后，肝腎細胞. dna經瓊脂糖凝膠電泳出現180一200bp整數倍的ona梯形帶， 19 . 09 / l一28 . 59 / l范圍內，亞稀褶黑菇提取液誘導肝腎細胞凋亡表現出時間和劑量依賴性 -
The biological characteristics and toxicity of russula subnigricans hongo was studied for the first time from ecology and morphologic characteristics and histology, the orthogonal experiment of the optimum culture condition, the analysis of components, apoptosis of the cells from little white rat liver and kidney induced by extract of russula subnigricans hongo, to the histopathologic changes observation of little white rat liver and kidney through ecological observation, light microscopy and scanning electron microscopy, reversed - phase high performance liquid chromatography, agarose gel electrophoresis, transmission electron micioscopy. the result showed as below : based on ecological observation of russula subnigricans hongo, its ecological environment was investigated in order to simulate its ecological environment when they are cultivated
利用菌種分離技術、光鏡技術、電鏡技術、高效液相色譜技術、毒理實驗技術、電泳方法等對亞稀褶黑菇（ russulasubnigricanshongo ）的生物學特性和毒性機理進行了研究，主要包括以下內容：亞稀褶黑菇的生態學和組織學研究、菌種分離培養、掃描電鏡觀察、成分分析、粗毒液誘導小自鼠肝腎細胞凋亡，小白鼠中毒后肝腎細胞透射電鏡觀察，研究結果如下： 1 -
After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。 -
Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained
Pcr產物經回收后，經瓊脂糖凝膠電泳鑒定后，與pcambia1303連接並轉化大腸桿菌dh5a ，陽性重組子經pcr和限制性內切酶酶切圖譜分析，表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。 -
I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]
從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。
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