agrobacterium 中文意思是什麼

agrobacterium 解釋
農桿菌屬，土壤桿菌屬
  1. In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays

    本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。
  2. Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility, with plasmid 3301 + 650, 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding, 98 by agrobacterium ). by cultivating them in blotting media containing basta 0. 4 mg / 1, we get 181 resistant leaves ( 98 by genegun bombarding, 88 by agrobacterium mediating )

    獲得轉基因雄性不育煙草為了證實hsp70反義cdna能創造雄性不育，我們將3301 + 650和3301 + 651質粒用基因槍和農桿菌介導法轉化煙草無菌發芽的葉片，共207片（基因槍109片，農桿菌98片） 。在含basta0 . 4mg l的篩選培養基上進行篩選，得到抗性葉片181片（基因槍93片，農桿菌88片） 。
  3. Because of the special biological structure of bryology, it was very difficult to transfer foreign gene into the protonema or gametophyte by agrobacterium - mediated transformation. protoplasts as acceptor, using direct dna transfer methods such as microprojectile bombardment and peg - mediated transformation is becoming a good way

    由於蘚類植物特殊的生物學結構用農桿菌侵染其原絲體或者莖葉體很難實現轉化，以原生質體作受體是蘚類植物轉化的常用途徑。
  4. Calli transformation of maize inbred via agrobacterium

    農桿菌介導的玉米自交系愈傷組織的轉化
  5. The results also revealed that it was a reasonable choice to use carbenicillin as the antibiotics of inhibiting growth of remnant agrobacterium after co - culture, and when hygromycin was used as the selective agent, continuous selection at low concentrations ( 50 ~ 150mg / l ) produced the highest numbers of transgenic plants without escapes

    試驗表明，在農桿菌介導高羊茅遺傳轉化中，抑菌劑宜選用梭節青霉素;以潮黴素作為選擇劑時，採用低濃度（ 50一15om留l ）連續篩選的方式比較合適，在該方式下，獲得的轉基因植株較多。
  6. Plantlet regeneration and agrobacterium - mediated transformation of peanut with epicotyl as explants

    花生上胚軸為外植體的植株再生及轉化研究
  7. A new transgenic system of gerbera mediated by agrobacterium tumefaciens

    小細胞團為受體的根癌農桿菌介導的非洲菊基因轉化體系
  8. Transformation of glycyrrhiza inflat bat by agrobacterium rhizogenes r

    發根農桿菌轉化脹果甘草的研究
  9. The suitable concentration of the selective agent kanamycin is 50mg. l - 1. the duration of infection and co - culture with agrobacterium influences soybean transformation evidently

    農桿菌侵染時間和共培養時間對大豆的轉化有明顯的影響，侵染時間以15min為宜，共培養時間以3d為宜。
  10. Therefore, toreniafournieri is a very suitable model plant for studies of double fertilization in living material. we transferred the fusion gene ofgfp : mtn to toreniafournieri by leaf disc transformation mediated by agrobacterium and gain transgenic plant

    我們將嵌合基因gfp : mtn通過葉盤法導入藍豬耳，探索了藍豬耳遺傳轉化的方法和轉基因苗的再生條件，建立了成熟的藍豬耳轉化系統。
  11. This paper describes a strategy that has developed to transfer the cdna of tobacco mnsod gene into the commercially important breeding line - baoding alfalfa via agrobacterium infection. transgenic alfalfa plants have been generated that overproduce a nicotiana plumbaginifolia l. manganese superoxide dismutase ( mnsod ). the results domenstrated that baoding alfalfa is an important breeding line which easily amenable to genetic transformation

    本研究採用我國農藝性狀優良的豐產苜蓿品種保定苜蓿，通過農桿菌介導的轉基因方法，使用特定的質粒載體pchlsod將煙草mnsod基因的cdna序列導入保定苜蓿中，說明保定苜蓿是一種易於遺傳轉化的優良苜蓿育種品系。
  12. A transcriptional fusion between 35s promoter and nearly full length hag1 cdna was constructed and transformation was performed through agrobacterium using root expantso the first whorl organs of transgenic plants flowers are carpel - like organs with stigmatic papilllae and a few ovules

    轉基因植株花的表型類似與ap2突變體，其第一輪花器官萼片具有心皮的特徵，頂端產生柱頭狀的乳突，邊緣有胚珠產生。
  13. As a popular vegetable, tomato is rich in vatamin a, b, p and other nutrients ; among them, lyxopene can prevent prostate gland tumor. so introducing stilbene synthase gene into tomato to get new health - protection tomato will have important social and economic effect. our research is made up of four parts ; the cloning and sequencing of stilbene synthase ; tomato genetic transforming mediated by agrobacterium - lba4404 ; quantification of resveratrol in transgenic tomato by hplc analysis

    本研究包括四個方面的內容：芪合酶基因（ stilbenesynthasegene ）的克隆與全序列分析；芪合酶基因（ stilbenesynthasegene ）植物表達載體的構建；農桿菌lba4404介導的番茄遺傳轉化： hplc技術分析轉基因植株中表達白藜蘆醇的含量。
  14. Using the leaves of " royal gala " as the explants, both vectors were transformed via agrobacterium - mediated transgene system. some transgenic plants have been obtained now

    目前己經獲得了2個株系的反義轉基因植株和3個株系的正義轉基因植株，轉化頻率為0
  15. As part of the project to improve isoflavone contents of legume forage by introducing isoflavone synthase ( ips ) gene, in this work, we have established the agrobacterium - mediated transformation system of white clover

    A311帶有pbi121質粒，該質粒攜有gus基因，其啟動子為35s啟動子，篩選標記基因為npt 。
  16. We produced transgenic tomato plants by an agrobacterium tumefacien - mediated method. pcr - southern blot and rt - pcr revealed integration of afgf gene in the plant genome and successful its transcription in transgenic plant cells

    我們利用農桿菌介導的轉化方法獲得了afgf轉基因番茄植物，並且通過pcr - southern 、 rt - pcr證明了afqf在宿主基因組中的整合及表達。
  17. As explants, were infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring the expression vectors pbgsb and pbglsb respectively and co - cultured for 3 days in darkness on the culture medium ( ms + 0. 1 mg / l 6 - ba + o. l mg / l naa + 0. 7 % agor ph 5. 7 )

    Ling inaa ）中暗培養三天後，移入加入抗生素100mg lkan和500mg幾cr的誘芽培養基，于組培室中進行誘芽和抗性篩選。
  18. Leaves of tobacco ( nicotiana tabacum ) were wounded, infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring expression vector pbgsb and pbglsb and co - cultured for 3 days in darkness on the culture medium ( ms + 0. 5mg / l 6 - ba + 0. 7 % agor ph 5. 7 )

    4以煙草（品種sr ）無菌苗葉片為外植體，採用農桿菌浸染的葉盤轉化法，用構建好的植物表達載體對煙草進行遺傳轉化。外植體置於無抗生素的誘芽培養基（ ms 0
  19. The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present. in the second part of this experiment, the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens, and kanamycin resistant transgenic p lants were obtained by screening in selective condition

    本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因（ ssnhx1 ）的表達載體prok2導入大豆子葉節中，經過含km的篩選培養基連續篩選，獲得了ssnhx1轉基因植株，篩選劑卡那黴素的適宜濃度是50mg . l ~ （ - 1 ） 。
  20. Somatic hybrid cells were obtained between ethionine resistant cell line of astragalus adsurgens pall, and agrobacterium / " / z / zogerae. s ' - transformed cell line of medicago saliva l. using protoplast fusion by peg method

    通過peg誘導原生質體融合和雜種細胞的篩選培養，首次得到沙打旺（ + ）紫花苜蓿的屬間體細胞雜種。
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