allantoic fluid 中文意思是什麼
allantoic fluid
解釋
尿囊液-
Two ndv strains, lasota and f48e9, were propagated in 9 - day - old embryonated specific - pathogen - free ( spf ) eggs respectively for 36 hours at 37, then these chicken embryos were placed at 4 for 12h before the allantoic fluid was gained
首先，將ndvlasota株和f48e9株分別接種於9日齡spf雞胚，於37孵化36h ，置4 12h后收獲雞胚尿囊液。 -
Heamagglutination tests were applied to detect virus in allantoic fluid of chicken embryos which were infected by b95 gathered from the vaccinated chickens " cloacal and oral cavity. the results show that the virus may be detected from 2 days to 11 days after the chicken being vaccinated. the hi antibodies were measured by heamagglutination inhibition tests. there is no significant difference between the immunized and the control chickens which were fed in one case. chickens were immunized with b95 by different immunization meathods or with different vaccines by the same meathod. lt is demonstrated that eyedrop, drinking water, spray or muscle injection all can stimulate good effects, but eyedrop and spray seem to be the best meathods. b95 immunized chicken have relatively higher hi titers and it also can last for a longer time than others
但如果兩者相隔10天以上免疫， b95免疫不受h120的影響；如果同時免疫b95和h120 ，加大b95的免疫劑量也能獲得良好的免疫效果。用棉拭子采b95免疫雞口腔、泄殖腔的分泌液，檢測其中病毒的存在，結果免疫后2 11天雞口腔和泄殖腔中均有病毒的存在，說明b95免疫雞帶毒時間長。研究結果表明， b95具有不受母源抗體干擾、 hi抗體產生快、水平高、持續時間長、同居擴散性強等特點，因此b95是一株優良的、具開發前景的新的新城疫疫苗株。 -
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。 -
The amniotic fluid and allantoic fluid of chicken embryo have been analyzed and the resonances of most substances in them were assigned by 1 - dimention and 2 - dimention nuclear magnetic resonance ( nmr ) methods. this work could be the basis of quantitative analysis of metabolites and studying the metabolites changing between the amniotic and allantoic fluid during the hatching process. also, it provided a new way to study the process of embryo developing
用一維及二維液體高分辨核磁共振（ nuclearmagneticresonance ， nmr ）方法綜合分析了雞胚羊水和尿囊液的成分，對其中的大多數譜峰進行了歸屬，發現了一些未經報道的小分子代謝物，為研究胚胎發育過程中各個胚囊中體液的交換和小分子的代謝以及進一步對發育過程中代謝物含量變化的定量研究打下了基礎，並為研究胚胎發育的過程提供了新的思路。 -
The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks
生物學特性：雞胚尿囊液經離心、磷鎢酸負染后，電鏡觀察該病毒為典型的冠狀病毒；該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用，當繼代到第5代后，胚體嚴重病變；病毒在雞胚中隨著接種時間的延長，其效價增高， 96h可達到48h的2倍；該毒株可在cef上生長，但不能形成明顯的蝕斑；經1胰酶處理后可凝集雞紅細胞；雞胚的第四代尿囊液病毒回歸動物體，病死雞腎臟呈典型的花斑腎，腺胃則未見肉眼可見的病變。
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