antibody chain 中文意思是什麼

antibody chain 解釋
抗體鏈
  • antibody : n. 【醫學】抗體。
  • chain : n 1 鏈子，鏈條；項圈；表鏈。2 連鎖；連續，一系列，一連串；（山）脈。3 〈常 pl 〉鐐銬；羈絆，拘束...
  1. This is the immunofluorescent appearance of the myocardium with antibody to lambda light chain. thus, this is " al amyloid "

    心肌中針對輕鏈抗體的免疫熒光圖。為免疫細胞衍生性澱粉樣變性。
  2. By sds - page and immuno - blotting, we found that a monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain antibody could react with cytoplasmic dynein intermediate chain - like protein at 67 kda in lily pollen. under confocal laser scanning microscopy after immunoflurescence labeling, we found that the dynein intermediate chain - like protein appeared punctated and was co - localization partly with microtubules in cytoplasm of lily pollen tube

    免疫熒光標記及激光共聚焦掃描顯微鏡觀察發現，類細胞質力蛋白中間鏈在百合花粉管中存在於顆粒狀細胞器上；免疫熒光雙標及激光共聚焦掃描顯微鏡觀察發現，百合花粉管中類細胞質力蛋白中間鏈和微管存在部分共分佈。
  3. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  4. Note the location of h and l chain complementarity - determining regions 3 ( h3 and l3 ) at the center of the antibody combining site

    表達產物經elisa和westernblot檢測證實具有與相應抗原結合的能力。本研究成功地構建了。
  5. By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles

    對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現，抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應；進而通過免疫金標結合電子顯微鏡觀察發現，大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上；統計結果表明，類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。
  6. The two isolates were positive reaction hi polymerase chain reactions with two pair of primers specific for alv - j and gave antigenically strong reaction hi the indirect fluorescence antibody assay ( ifa ) with alv - j specific monoclonal antibody je9. negatively - stained electron microscopic and immune - electron microscopic observation demonstrated that viral particles of the inner mongolia and shandong isolate of alv - j, respectively designated imc10200 and sdc2000 strain of alv - j, showed characteristic morphology of alv

    利用pcr和間接免疫熒光反應進行鑒定，兩株j亞群禽白血病病毒可以被兩對alv - j特異性引物擴增（特異條帶約2 . 2kb和545bp ） ，且在特異性單克隆抗體je9的間接免疫熒光檢測中呈現強陽性熒光反應。
  7. Ricin toxin has wide - range anti - tumor spectrum. however, because rta has no selectivity, it can damge normal ce lls too. these years, ricin and it ' s a chain ( rta ) have been used to conjugate with monoclonal antibody to prepare immunotoxins ( its )

    近年來，蓖麻毒a鏈可以與單克隆抗體相交聯而制備免疫毒素（ immunotoxin ， it ） ，免疫毒素既具有強大的腫瘤殺傷作用，同時又有抗體的特異性，可以將毒素分子靶向運送到腫瘤細胞，以減少對正常細胞的損傷。
  8. New york ( reuters health ) dec 19 - lactobacilli transformed to carry heavy - chain antibody fragments appear to protect against rotavirus infection, swedish investigators report in the december 1st issue of the journal of infectious diseases

    紐約（路透社健康版） 12月19日瑞典研究人員首次在12月傳染病雜志上發表報告稱：經改造攜帶重鏈抗體片斷的乳酸菌有抗輪狀病毒感染的作用。
  9. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  10. Because expressed in escherichia coli, ricin a chain is not degraded easily for its non - glycosylation. our experiments lay a foundation for the researches that rta conjugates a monoclonal antibody to synthesize immunotoxins and the immunotoxins are used for cancer - targeted therapy

    蓖麻毒素a鏈在大腸桿菌中表達，因無糖基化修飾，克服了進入體內后被迅速降解的缺點，為進一步與單鏈抗體基因嵌合表達形成單鏈免疫毒素，對腫瘤細胞進行導向治療研究奠定基礎。
  11. Construction and screening of phage display single chain antibody library against bursaphelenchus xylophilus cellulase

    抗松材線蟲纖維素酶單鏈抗體庫的構建及篩選
  12. H chain cdr3 region is thought to be the antigenic epitope of antibody of monoclonal antibody np30. therefore, anti - id antibody vaccine and antigenic epitope vaccine are important strategy in developing schistosomajaponicum vaccine

    Fv基因，且其表達產物保留了抗體的親和性和特異性，有可能替代雜交瘤細胞分泌的單克隆抗體np30作為免疫檢測試劑。
  13. Construction of murine phage antibody library and selection of n - peptide - binding single - chain antibodies

    小鼠噬菌體抗體庫的構建和n -肽結合單鏈抗體的篩選
  14. The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain

    為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。
  15. Using the monoclonal antibody to rev and the anti - sera against the rev env gp90 - gst fusion protein. the molecular cloned virus was detected by ifa. we also amplified the gp90 from the cells infected with the molecular cloned virus by polymerase chain reaction. all these results indicated the recombinant plasmid containing the total rev genome cdna is infectious

    對snv株前病毒全基因組cdna克隆進行酶切，將酶切產物分別克隆進puc18中，分別將各個亞克隆進行測序，按照酶切位點和已知的部分序列以及rev物理圖譜將測得的序列進行拼接，完成了rev全基因組序列。
  16. Amplification, cloning and sequence analysis of the light chain and the heavy variable region genes of monoclonal anti - idiotypic antibody np30 of schistosoma japonicum to amplify and sequence the gene of the heavy chain and the heavy chain of anti - idiotypic monoclonal antibody np30 of schistosoma japonicum

    2 、日本血吸蟲單克隆抗獨特型抗體np30的單鏈抗體kcfv ）對balb c小鼠誘導保護性作用研究為了研究日本血吸蟲抗獨特型抗體np30單鏈抗體作為日本血吸蟲病
  17. Cloning and expression of human anti - rhg - csf single chain antibody gene

    單鏈抗體基因的克隆和表達
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