antisense gene 中文意思是什麼
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Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。 -
Protective effect of c - fos antisense gene recombinant transfection on ischemic hypoxic cardiomyocytes
反義基因轉染對缺血缺氧心肌細胞的保護作用 -
Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato
核酶嵌合基因植物表達載體的構建及對番茄的轉化 -
To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated
但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。 -
They contained an antisense constructed for the spruce gene encoding ccr ( cinnamoyl alcohol dehydrogenase ), an enzyme of monolignol synthesis. the antisense rna method is a technique for reducing the expression of a resident target gene. the transgenic sublines were produced by particle bombardment at the dept of forest genetics, swedish university of agricultural sciences
本項目研究的目的就是採用瑞典農業大學構建的反義ccr基因轉導的挪威雲杉細胞愈傷組織，通過誘導產生再生植株，並檢測證實該基因是否表達及其它相關基因的表達狀況，為培育出低木質素的轉基因挪威雲杉新品種奠定物質和理論基礎。 -
Are the changes in signal transduction and gene expression related to the structural alteration of the sugar chain on some surface receptors resulting from the transfection with sense or antisense gnt - v
而細胞信號轉導的改變是否與細胞表面某些受體的糖鏈結構的改變（由gnt - v的正義或反義cdna轉染引起的）有關 -
In the presented study, using h7721 human hepatocarcinoma cell line transfected with sense or antisense cdna of gnt - v, the effects of gnt - v on signal transduction an d its mechanism as well as the alteration of gene expression were investigated. we expected to elucidate whether and how the transfected glycosyltransferase modulate the cell signal transduction and gene expression
本文以穩定轉染gnt - v正義或反義cdna質粒的h7721人肝癌細胞為材料，從下列四個方面對gnt - v影響信號轉導及其機制以及引起基因表達的變化進行了研究，企圖闡明糖基轉移酶轉染是怎樣調節細胞信號轉導的 -
Carnation flower vase life prolonged by transformation with antisense acc oxidase gene
基因導入獲得瓶插壽命長的香石竹植株 -
Cloning of rejection related gene and construction of antisense gene transferring vector
玉米溫敏核雄性不育相關基因克隆 -
Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis
分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。 -
Reverse malignant phenotypes of colonic carcinoma cells transfected with antisense gene to htrt
反義人端粒酶催化亞單位基因轉染對人結腸癌細胞惡性表型的逆轉作用 -
This vector, including sbe2b gene, was 6. 06kb long, containing endosperm specific promoter and a intron that enhanced the transcript efficiency. both sense and antisense cdna were transformed to upregulate and downregulate amylose content and relative branching characters
正向連接的表達載體用於增加胚乳中支鏈澱粉的含量，反向連接用於減少胚乳中支鏈澱粉的含量，增加直鏈澱粉的含量。 -
Antisense nucleic acid technique is a method using single - stranded complementary sequences with specificity assigned by watson - crick base - pairing targeted specific messenger rna or dna to block expression of target gene
反義核酸技術是根據堿基互補原理，使用與目標靶的遺傳物質（ mrna或dna ）特定互補的核酸片段封閉基因表達的技術方法。 -
An expression vector with fragment 9 in antisense orientation was constructed to block the expression of the relevant gene ( fragment 9 related gene, fnr gene ) in vero cells. interestingly, we found that the nontargeted mutation frequency induced by mnng was increased significantly, implicating that the product of the blocked gene may be involved in the inhibition of nontargeted mutation
利用反義核酸技術構建含反向插入9號片段的真核細胞表達重組體並轉染細胞，以獲得反義rna阻斷vero細胞中相應基因的表達，發現mn 』 ng誘發的非定標性突變頻率顯著增高，提示被阻斷的相關基因的表達產物可能參與抑制非定標性突變的發生。 -
Construction of the recombinant adenovirus vector carrying antisense multidrug resistance gene
靶向肝癌細胞的重組腺相關病毒載體的構建 -
Construction of recombinant adeno - associated virus vectors carrying double gene of antisense multidrug resistance - associated protein and antisense multidrug resistance
1雙耐藥基因的重組腺相關病毒載體的構建與鑒定 -
In the experiment, we have explored optimal conditions for the transformation of torenia fournier, and transformated it with antisense acc synthase gene to get transformated torenia fournier plants with flower longevity extension and more tolerance to abiotic stress
本研究探索和完善了藍豬耳遺傳轉化條件，並將反義acc合酶基因轉化藍豬耳，以期獲得延長花壽命，和有較高抗性的轉基因藍豬耳植株。 -
In this research, we constructed the expression vectors, which contain exogenous gene chalcone systhase ( chs ) antisense gene of gerbera hybrida and transformed it into agrobacterium lba4404 through freezing - melting. an efficient regeneration system and transformation system have been estadblished on leaf discs of torenia fournieri lind
以藍豬耳為主要研究對象，建立了高效的藍豬耳離體再生體系，並以藍豬耳葉片為轉化材料，用帶有目的基因的農桿菌感染建立了藍豬耳的遺傳轉化體系。 -
Genetic transformation of the acc oxidase antisense gene to cucumis melo l. cv. hetao via agrobacterium tumefaciens
氧化酶反義基因轉化甜瓜品種河套蜜瓜的研究 -
The elisa and western blot analysis indicated that the reaction between annserum and antigen was high specifically. to block the annfaf expression in strawbern. " fruit, the antisense gene of annfaf
為獲得annfaf基因的表達缺陷型植株，以研究該基因在草莓果實成熟過程中的調控作用，本研究構建了annfaf反義融合基因的植物重組表達質粒prok - annfaf 。
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