antiviral protein 中文意思是什麼
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The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。 -
To find out the difference between mut + and muts recombinants we compared their expression of pokeweed antiviral protein in the same conditions
在相同的培養條件下比較了mut ~ +和mut ~ s重組菌株表達pap的異同。 -
Someone tried this way but the yield is low. francis expressed truncated mutant pokeweed antiviral protein gene in pichia pastoris
目前已經有人在這方面做出了嘗試，但蛋白表達產量還不高。 -
Many studies revealed that pokeweed antiviral protein ( pap ) can control plant virus effectively
許多研究發現美洲商陸抗病毒蛋白（ pokeweedantiviralprotein ， pap ）對植物病毒具有良好的控制效果。 -
Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting
本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。 -
Sds - page results showed that as to mut + recombinant highest yield was obtained after 4 days inducing and with the culture time prolonged it reduced. pokeweed antiviral protein gene expressed well when methanol concentration reached 10g / l. pokeweed antiviral protein obtained high yield in thin acidic culture medium ( ph6. 0 - 6. 4 ) and its quantity in total mass of secrete protein exceeded 30 %
Sds - page分析結果表明， mut ~ +組菌株在甲醇誘導第四天後pap在培養液中積累量達到最高水平，延長培養時間會導致產量下降；在10g / l的甲醇濃度誘導下， pap的表達量達到最高；培養基ph值在偏酸性條件下（ 6 . 0 - 6 . 4 ） pap的表達量都維持在較高的水平。 -
Antiviral protein, avp
抗病毒蛋白 -
Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein
本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。 -
The recombinant protein exhibited specific binding to antiviral antibodies of h5 subtype and has no cross - reaction with h7 and h9 subtype
分別用親和層析以及電洗脫兩種方法對gst - ha1融合蛋白進行了純化，都獲得了理想的純化結果。
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