autoradiography 中文意思是什麼
autoradiography
解釋
放射顯跡圖-
The protein nucleic acid complexes were identified by autoradiography for 48 h. the psg5heo and igfbp - 6 promoter pcat vectors were transfected into cos - 7 cells with iipofectamin2000 ; these cos - 7 cells where treated with 1 jxm e2, with lonm tcdd and with both lonm tcdd and 1 jxm e2, individually ; the cat protein was examined by cat - els a. results : the congenital skeletal defects in fetal rats were induced with 10 - 15ug / kg tcdd or with 100 - 140 mg / kg ra with dosage - dependence effect. the igfbp - 6 mrna in rat calvaria tissue and mc - 3t3 - el cells were increased with tcdd and ( or ) ra, whereas the cell proliferation and igf - 2 mrna in mc - 3t3 - el cells was decreased in the status of e2
結果: tcdd在大鼠胚胎發育時期具有骨骼致畸作用，骨骼發育畸形包括:內翻足、脊柱裂、顱面骨發育缺陷、無尾畸形等;隨著tcdd濃度的升高（ 10一15林擴kg ） ，致畸率明顯上升（ 14 . 7 %一24 . 6 % ） ; tcdd和ra聯合用藥可以增強對大鼠骨骼發育的致畸效應; e :對成骨細胞內igf一2基因有正性調節作用，對igfbp一6基因有負性調節作用， tcdd可以抑制e :對細胞內xgf一2和igfbp一6的調節作用。 -
Autoradiography a technique whereby a tin slice of tissue containing a radioactive isotope is placed in contact with a photographic plate
放射自顯影術：將含有放射性同位素的一小薄片生物組織與感光層接觸的一項技術。 -
Mrna from the mitten crab ovaries at stages ii and iii were made as 33p probes by reverse transcription reactions respectively, then two identical membranes were hybridized with the two kinds probes. washing, pressing and autoradiography were performed before signals scanning and analysis. in total, 167 clones whose signal difference was > 2 fold were obtained
分別將中華絨螯蟹期和期卵巢的mrna反轉錄標記成~ （ 33 ） p探針，然後將2種探針分別與2張相同的膜雜交，經洗膜、壓片和放射自顯影后進行信號的掃描和分析。
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