avirulent strain 中文意思是什麼
avirulent strain
解釋
減毒株，無毒株-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。 -
In recent years, the outbreak of nd appear some new characters, it is reported that the amount of immunity defeat on nd increased, even chicks with high hi titer can be infected by ndv virulent strain. the gene - engineering vaccine is badly needed to control nd outbreak. so newcastle disease virus strain b95, a naturally avirulent and heat stable strain, was introduced from australia
新城疫是國際獸疫局公布的16種烈性傳染病之一。近年來，新城疫的爆發出現了許多新特點，免疫失敗報道增多，甚至抗體水平較高的雞群也發病。為控制新城疫的爆發，急需研製基因工程苗。 -
From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain
本實驗採用spf雞胚及雞胚原代成纖維細胞，從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞，並且這種凝集可以被特異性抗血清所抑制。
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