bacmid 中文意思是什麼
bacmid
解釋
桿粒[帶有桿狀病毒基因組的質粒，可在細菌和昆蟲細胞之間穿梭-
The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme, were linked by means of t4 dna ligase and transformed into e. coli jm109 permissive cells, yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid
將重組質粒pugedna與轉移載體pfastbacldna用bamhi和ecori雙酶切處理， t _ 4dna連接酶連接，用連接產物轉化大腸桿菌jm109感受態細胞，得到重組轉移載體質粒pfastbac - gedna 。 -
Transfection of the gp64 - mi and reintroducing hal33 gene bacmid dna into sf9 cells shown that hal33 can rescue the gp64 null bacmid in sf9 cells
對該重組病毒bv的westernblot檢測，結果表明fi和fz同時存在並以二硫鍵相連。 -
The interest gene was inserted in the - tha l. tho 1 multiple cloning sites of donor plasimd pfastbachtb of baculovirus expression system. after analysis by restriction endonuclease and pcr, the recombinant donor plasmid gpl - fast was transforn1ed to the competent celi dhi0bac which contalns the bacmid and the helper plasmid, the recombinant bacmid gpl - bac was acquired which would express the vpl of gpv strain h l
同時將該目的基因插入到桿狀病毒表達系統的供體質粒pf _ （ ast ） b _ （ ac ） htb的xba 、 xho多克隆位點間，經酶切、 pcr鑒定后，將重組的供體質粒gp1 - fast轉化到含有桿狀病毒和輔助質粒的dh10b _ （ ac ）感受態細胞中，獲得了表達gpvh1株vp1的重組桿狀病毒gp1 - bac 。 -
I ) two mutants ( hpab - 38 and hpab - 34 ) were designed based on the three - dimension structure of hpab - constructed by protein homology modeling method, ii ) the mutant molecules were generated by pcr and inserted into pfast hta donor plasmid, the later was then transformed into escherichia coli dhlobac to generate recombinant baculoviruses dna by site - specific transposition of an expression cassette into a baculovirus shuttle vector ( bacmid ) propagated in e. coli. the recombinant bacmids were isolated and transfected into insect sf - 9 cells to reproduce baculoviruses
4 . h隊b一p及其突變體高效表達工程菌的構建:將用pcr擴增的h隊b一p 、 h獄b一p35 、 h隊b一p34基因，分別與pinpointxa一3質粒連接，轉化jm109大腸埃希菌，獲得的工程菌在表達融合蛋白時不穩定，第5代后即見不到表達條帶。
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