bait protein 中文意思是什麼

bait protein 解釋
引誘蛋白
  • bait : n 1 餌；引誘物；誘惑。2 （路上的）休息（或進食）。vt 1 把餌裝到…上；引誘。2 （在路上）喂喂（馬）...
  • protein : n. 【化學】朊，蛋白（質）。
  1. The targeted disruption of mouse rbp - j revealed that homozygous null mutants die before 10. 5 days postcoitum ( dpc ) with various abnormalities, including growth retardation and defects in the central nervous system and somites. in order to assess the function of rbp - j, a yeast two - hybrid screening using rbp - j as a bait protein was performed and a nuclear matrix protein, mint was isolated

    Sdpc胚胎和人的hela細胞cdna文庫中篩選到一個可以與rbp斗相互作用的新蛋白ram7 ，后發laram7與mmt （ msxz inter肌tingnucleartargetprotein ）為同一蛋白。
  2. To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively

    鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。
  3. We firstly used g protein a subunit as bait

    首先以g蛋白亞基為誘餌構建了誘餌蛋白表達載體。
  4. The study on the function and mechanism of phrip1 is important for clarifying how the cell plate and cell wall form in plants. in this study, full length of phrip1 is amplified by pcr and ligated into pks plasmid, then the bait plasmid, peg202 - phrip1, is constructed. the inseret gene are sure to be translated into the right fusion protein through its sequence. in the yeast two - hybrid system, the bait plasmid ( peg202 - phrip1 ) and a reporter plasmid ( psh18 - 34 ) are introduced into the yeast ( egy48 ) by co - transformation. then cdna library ( which is in pjg4 - 5 ) is screened and two genes are obtained. the two insert gene fragments are sequenced. one of them is plastocyanin, the other is putative photosystem i reaction center subunit ii precursor, both of them are the necessary components of photosynthetic chain

    成膜素相關蛋白1 （ phrip1 ）是一個含608個氨基酸的蛋白質，它對于植物胞質分裂中細胞板的形成起到了十分重要的作用。研究phrip1的功能和機制，對在分子水平上闡明植物細胞板以及細胞壁形成的機理具有重大的生物學意義。在本實驗中，根據phrip1的序列設計引物對其進行pcr擴增，得到該基因后將其連接到了pks質粒上，並進一步構建成了誘餌質粒peg202 - phrip1 。
  5. Using yeast two - hybrid system with lim domain protein kyotl as a bait, we have isolated an alternatively spliced form of human tight junction protein 2 - - - - - - zo - 2 - i3. the tight junction ( or zonula occludens ) is the most apical junction between neighbouring epithelial cells or endothelial cells

    53為深入研究kyoth在notch rbpjk途徑中的可能作用及其作用機制，本實驗室以kyoth為誘餌蛋白通過酵母雙雜交得到人類緊密連接蛋白zoe 。
  6. In order to assess the function of rbp - j, a yeast two - hybrid screening using rbp - j as a bait protein was employed and a lim protein, kyot, was isolated

    Kyot是一種lim結構域蛋白，有兩種亞型kyoti和kyotz ，在染色體上由同一基因編碼
  7. These results suggest that kyot plays an important role in the development of testis and spermatogenesis. 2 we performed yeast two - hybrid screening of a cdna library from human lymph nodes using kyot2 as a bait protein. 42 clones were gotten after 5xl06 were screened by four kinds of nutrition limitation and p - galactosidase assay, 22 clones were got after restriction of positive clones, at last, 13 genes were got by sequence assay including rbp - j known as the interacting protein with kyot before and two novel genes

    對此22個克隆進行序列分析，共獲得13種基因，其中包括已知的kyot相互作用蛋白rbpj和兩個未知基因，也包括在哺乳動物睪丸中特異3第四軍醫大學博士學位論文性表達的蛋白piasi ，同時還篩到了具有可與lm結構鞠互作用的pdzdomain分子緊密連接蛋白2和兩種同屬于kg家族的蛋白ringi和polycomb2
  8. In order to assess the function of kyot2, northern blot, immunohistochemical sabc and in situ hybridization methods were used to find out the expression of kyot and kyot knockout mice which were kept in our laboratory were analyzed. moreover, we employed a yeast two - hybrid screening using kyot2 as a bait protein and got 13 interacting proteins. the function of a novel protein of them, kbp was investigated

    為了進一步研究kyot的功能，我們利用northern印跡、兔疫組織化學等方法對kyot在體內的分佈進行時空定位，並利用本室懈的kyot基困剔除小鼠分析其表型，同時採用酵母雙雜交篩到tkyoth相互作用的13種分子，並對其中的一個新分子kbp進行了功能的研究
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