binding assay 中文意思是什麼

binding assay 解釋
結合測定
  • binding : adj 1 縛[捆、綁]…的；黏合的；系連的，連結的。2 有束縛力的，有拘束力的，附有義務的。3 〈口語〉引起...
  • assay : n 1 化驗；分析；鑒定，測定，驗定。2 被分析物，被化驗物。3 化驗結果，化驗報告。4 〈古語〉企圖，嘗...
  1. A competitive binding assay used to determine hapten iaa with the aid of hrp - iaa conjugate has been developed. it was based on the ordered, stable mercapto self - assembled monolayer formed on gold surface, which immobilized igg with covalent bond

    方法基於金基底上形成的均一、穩定、有序的巰基乙酸自組裝單層膜，在edc 、 nhs活化下，能以共價方式固定抗體。
  2. There were some degration in the purified protein, but gst - ap - 2 a still had the dna binding activity in the gel shift assay. the gst - testin and gst - antn1 were used for immunolize rabbits

    雖然所提取的融合蛋白gst - ap - 2出現較多降解，但是電泳遷移率變動分析顯示其仍然具有良好的dna結合活性。
  3. To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively

    鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。
  4. If determination of an analyte concentration is required, the assay system based on immunochromatography can be combined with a detector that is capable of precisely quantifying the binding complex formed as a result of the test, although the system is commonly fabricated in a stand - alone format

    如果需要測量分析物的濃度，建立在免疫層析基礎上的檢測系統可與監測器相結合，這個監測器能準確定量形成的作為測試結果的結合物，雖然系統一般以單獨站立的形式製造出來。
  5. Besides, vgb gene expression also increased the chitinase secretion. in order to make the vitreoscilla hemoglobin gene express in bacillus subtilis, an inducible promoter ( levansucrase ) was cloned from b. subtilis wb600 and ligated with a promoter - less vgb gene. the resulted gene is called sacvgb and was demonstrated to express in e, coli by sds - page and carbon monoxide binding assay

    由於vgb基因啟動子不能在枯草桿菌中啟動表達，因此，根據已發表的果聚糖蔗糖酶基因（ sacb ）序列設計引物，從枯草桿菌wb600總dna中擴增出該基因的啟動子片段，然後將其與vgb基因編碼區及終止子序列相連，成功地組建了sacvgb融合基因。
  6. In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot

    以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。
  7. Pu xiaochun, ji weizhi, yang shangchuan, chen jianchun, zuo roujin and shang enyuan 1994 correlation of zona - binding with oocyte maturation and sperm motility in rhesus monkeys by hemizona assay

    普曉春、季維智、楊上川、陳建春、鄒如金、商恩緣1994獼猴卵母細胞成熟度和精子活力與精卵結合的關系。
  8. Pu xiaochun, ji weizhi, yang shangchuan, chen jianchun, zou rujin, and shang enyuan 1994 correlation of zona - binding with oocyte matoration and sperm motility in rhesus monkeys by hemizona assay

    普曉春、季維智、楊上川、陳建春、鄒如金、商恩緣1994獼猴的半透明帶分析法精子活動度和卵母細胞成熟度對精卵結合率的影響。
  9. Rafnar b, traustadottir k, sigfusson a, et al. an enzyme based assay for the measurement of complement mediated binding of immune complexs to red blood cells. j immunol methods 1998 ; 211 : 171 - 181

    郭峰，盧培恩，王海濱，等.新鮮血對癌細胞快速自然免疫反應的發現與實驗方法的創建.深圳中西醫結合雜志1999 ; 9 : 7
  10. The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga

    通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。
  11. To identify the interaction between kyot2 and zo - 2 - i3, yeast two - hybrid system, purification of kyot2 protein and gst pull - down assay were performed in the experiments. after kyot2 and zo - 2i3 exchanged their vectors, yeast two - hybrid test revealed physical binding of the two proteins

    本實驗通過酵母雙雜交及gstpull downas腳驗證了kyotz蛋白與zoz蛋白在體外的相互作用，並通過酵母雙雜交實驗初步確定其相互作用的位點。
  12. Proteinmicroarrays can be based on any ligand - binding assay that relies on theformation of product with an immobilized capture molecule and a targetmolecule present in the solution

    蛋白質晶元可以基於任何配體結合實驗，靠產品形成一個固定捕捉目前在分子和分子靶解
  13. The vhb expression in the strain hv was investigated by co - binding assay. in flask culture, the strain hv had almost cell densities compared with e. colihms174 in the early phase of the growth

    在搖瓶中培養，發現在培養前期， hv的生長與無vhb基因的對照菌e . colihms174相差不多，到培養後期時， vhb基因整合菌hv才略有生長優勢。
  14. Methods receptor binding assay ( rba ) was adopted to measure the distribution of pgr and er in liver cancer and tissue in its vicinity in 63 patients with primary liver cancer ( plc ) undergone laparotomy and in non - tumor liver tissue in 7 cases with gastric carcinoma

    方法採用放射性配基結合分析法（ rba ）測定了63例行手術切除的肝癌組織、癌周組織及7例非肝癌肝組織的雌、孕激素受體的含量並結合臨床資料進行統計分析。
  15. By yeast two - hybrid assay, aes was found to interact with gp130 intracellular region through its conserved q domain. results from the yeast two - hybrid assay, gluthione s - transferase fusion protein pull - down assay and immuno - co - precipitation assay indicated that the q domain of tle1 is capable of binding gp130 intracellular domain, and the intracellular membrane proximal region of gp130 containing conserved boxl and box2 motifs seemed essential for this interaction. to investigate the consequence of this interaction, tle1 - gfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with gp130 expression vector

    在通過酵母雙雜交分析確定aes通過q結構域與sp130分子胞漿區結合的基礎上，為確定tle1分子是否也能通過保守的q結構域與gp130分子胞漿區結合，我們通過pcr擴增編碼gp130胞漿區與tleq分子不同結構域的cdna ，構建了含有這些不同結構域的酵母雙雜交載體，通過酵母雙雜交分析證實： tle1分子通過其氨基端的q結構域與gp130分子胞漿區近膜段結合。
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