blot hybridization technique 中文意思是什麼
blot hybridization technique
解釋
吸印雜交技術,印跡雜交技術- blot : n 1 墨污，墨漬，污點，污斑。2 瑕疵；恥辱，污名。3 〈古語〉塗去，抹去。vt （ tt ）1 用（墨水等）弄...
- hybridization : 混成作用
- technique : n. 1. （專門）技術；（藝術上的）技巧，技能。2. 手法〈如畫法，演奏法等〉。3. 方法。
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Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city
最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。 -
Was first processed by dgd embedment and embedment - free technique and general technique for em morphology. a perinuclear structure consisted of interacted filaments we called lamina - like structure was observed. then using western blot assay, we found a lamin - like component band of 68kd protein in the three - step - fractionated cells. to investigate the distribution of the lamin - like protein in cells, a immunofluorescence experiment for in situ hybridization was designed using goat anti - lamin protein antibodies as the probes. the results revealed that the positive reactivity presented at different part of the cells. the perinuclear cross - actions were distinct, and cross - action with the oral apparatus and the cortex were also obtained
本文以dgd包埋去包埋技術對草履蟲的核纖層通過透射電鏡和免疫熒光分子雜交等技術進行了觀察。結果顯示，在核周存在由10nm纖維組成的核纖層免疫熒光結果表明，在核周呈陽性反應，並在其表皮口器等部位呈交叉的陽性反應蛋白分子雜交的反應帶在68kd處呈陽性反應。
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