blotting 中文意思是什麼

Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility, with plasmid 3301 + 650, 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding, 98 by agrobacterium ). by cultivating them in blotting media containing basta 0. 4 mg / 1, we get 181 resistant leaves ( 98 by genegun bombarding, 88 by agrobacterium mediating )

獲得轉基因雄性不育煙草為了證實hsp70反義cdna能創造雄性不育，我們將3301 + 650和3301 + 651質粒用基因槍和農桿菌介導法轉化煙草無菌發芽的葉片，共207片（基因槍109片，農桿菌98片） 。在含basta0 . 4mg l的篩選培養基上進行篩選，得到抗性葉片181片（基因槍93片，農桿菌88片） 。

Blotting paper soaks up ink.

吸墨紙能吸墨水。

Test method for ink absorption of blotting paper

吸墨紙的墨水吸收性試驗方法

Standard test method for ink absorption of blotting paper

吸墨紙的墨水吸收性標準試驗方法

Paper and board. characteristics of blotting paper

紙.吸水紙特性

Water will permeate blotting paper

水能滲透吸水紙

It is not the real tatoo, it is only the blotting paper, haha !

那不是真的紋身，那次我去派對留下的印水紙!哈哈

I suggested to him about a transparent show cart with two smart girls sitting inside writing letters, copybooks, envelopes, blotting paper

我曾向他建議，讓兩個美女坐在一輛透明的陳列車里寫信，並擺上筆記本信封和吸墨紙。

The insulin - like growth factor - 2 ( igf - 2 ) and igf conjugated protein - 6 ( igfbp - 6 ) mrna level in rat calvaria bone tissue and mc - 3t3 - el cells were detected by northern blotting analyses and reverse transcription polymerase chain reaction. the estrogen responsive element ( ere ) in igfbp - 6 gene promoter was identified and involved in tcdd - reduced regulation of the gene expression by electromobility shift assays ( emsa )

正常胎鼠頭蓋骨組織igf一2mrna呈高水平表達狀態，而igfbp一6mrna的水平較低; ccf胎鼠頭蓋骨骨組織內igf一2mrna的表達較正常胎鼠降低， igfbp一6mrna的表達則明顯升高; atra和e :聯合應用時， atra可以抑制雌激素對細胞內igf一2和igfbp一6的這種調節作用。

This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting

本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基

In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。

However, it is necessary to acquire the antibody or the antiserum, which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin, using the special immune serum of pea ferritin, the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation, eljsa and western blotting

利用免疫檢測技術進行植物轉基因的表達檢測是一種簡單、靈敏、快速、可靠的方法，但其前提條件是要有與轉基因植物目的基因表達的蛋白質發生特異性免疫反應的抗體或抗血清。根據植物鐵蛋白之間有高度同源性和交叉免疫反應的特性，利用特異性的豌豆鐵蛋白抗血清，就可通過免疫沉澱、 elisa或western雜交等免疫檢測方法進行植物鐵蛋白含量等的檢測，從而更好地進行轉基因方面的研究。

It was the perfect setting for the blotting out of a too inquisitive foreign devil.

要結果掉一個過分好奇的洋鬼子，這是個好去處。

To verify the integration of st901 gene into transgenic plants genome, the stable transgenic plants were analyzed by pcr and southern blotting. the aberrant phenotypes were observed in pollens and anthers of the transgenic plants. most of pollen grains in the transgenic plant were distorted, shrunken, invagination and not stained with the solution of acetocarmine

通過對轉基因植株花粉、花藥形態的觀察和花粉活力的測定，表明st901啟動子驅動的st901基因在轉基因植株中的表達造成花粉嚴重敗育，花粉粒皺縮，扁癟、塌陷，缺乏內容物；轉反向表達載體的馬鈴薯花粉育性僅為對照的5 . 2 ，育性下降94 . 8 。

By sds - page and immuno - blotting, we found that a monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain antibody could react with cytoplasmic dynein intermediate chain - like protein at 67 kda in lily pollen. under confocal laser scanning microscopy after immunoflurescence labeling, we found that the dynein intermediate chain - like protein appeared punctated and was co - localization partly with microtubules in cytoplasm of lily pollen tube

免疫熒光標記及激光共聚焦掃描顯微鏡觀察發現，類細胞質力蛋白中間鏈在百合花粉管中存在於顆粒狀細胞器上；免疫熒光雙標及激光共聚焦掃描顯微鏡觀察發現，百合花粉管中類細胞質力蛋白中間鏈和微管存在部分共分佈。

Pupal hemolymph infected with recombinant virus bmpak - hbmp and bmpak - hbm showed four protein bands of about 31, ' 30, 28 and 25 kd in the western blotting profiles, due to the two atg initial codons at the 5 " ends of both pres2 and s gene

Western雜交結果表明，感染bmpak hbmp和bmpak hbm的家蠶蛹血淋巴中均有4種不同分子量的蛋白質和鼠抗人的一抗發生免疫反應，大小約分別為31kd 、 30kd 、 zskd 、 25kd 。

These results demonstrated that merely examining the segregation of reporter gene in pollens is a reliable method that could avoid the laborious testing process of the pure line from transformed plants ; ( 3 ) the transcript levels of leetrl and leetrl were checked by northern blotting analysis

（ 3 ）兩個基因在反義植株里的表達都受到不同程度的抑制。在反義leetr1植株里， leetr1表達被抑制后， leetr2的表達也受到不同程度的抑制。同時，反義leetr2植株里也有相似的結果。

Northern blotting analysis showed that cryld mrna was more stable than crylab mrna, however, cryld mrna formed later 3h and less 3. 7 times than crylab mrna during the mid - phase of sporulation. moreover, crylab mrna could keep stable and large amount during the post - phase of sporulation. this suggested that difference of transcriptional efficiency and initiate time might be main reason for the difference of expression of crylab and cry id

結果表明：基因cry1dmrna的形成比基因cry1ab的mrna滯后3小時，且基因cry1d形成mrna的量很低，產生過程很平穩，在芽胞形成中期比cry1abmrna低3 . 7倍； cry1abmrna含量在芽胞形成前期高於後期，在後期仍能大量持續穩定地轉錄。

As the ancient clerk deliberately folded and superscribed the note, mr. cruncher, after surveying him in silence until he came to the blotting - paper stage, remarked

老行員仔細折好字條，寫上收件人姓名。克朗徹先生一聲不響地觀察著他，在他吸干墨水時說：

Sds - page and western blotting of plasmodia of physarum polycephalum showed that cyclin b was present in the plasmodia of s phase, g2 phase, prophase, and metaphase. the molecular weight of cyclin b in physarum polycephalum was around 62kda. cyclin b was gradually accumulated from s phase to metaphase, and reached the maximal level at metaphase, however disappeared in the plasmodia of anaphase and telophase

Westernblotting分析結果表明， cyclinb存在於s期、 g _ 2期、前期和中期的原質團中，分子量約為62kda ， cyclinb的含量從s期到中期逐漸增加，特別是有絲分裂前期增加明顯，中期原質團cyclinb含量最高，后末期原質團中沒有cyclinb 。