bmn 中文意思是什麼

bmn 解釋
中腸腺壞死桿狀病毒
  1. ( 3 ) the chromosomes obtained by air - drying and chromosomes from the silkworm genital gland are similar in shape. but the numbers and the length of the chromosomes are obviously different. the chromosomes obtained from the genital gland have little difference in length, while the chromosomes of bmn cell are different in length very much

    但是，在染色體的數目上有顯著的差異。另外，染色體間的長短差別也非常明顯。由家蠶生殖腺得到的減數分裂中期染色體和胚胎得到有絲分裂的中期染色體，其染色體長短差異不明顯，而binn細胞的中期染色體長短非常不一致，差異非常顯著。
  2. Then we transfected the plasmid to the bmn cells through lipofectin

    在不同的時間，收集細胞上清。
  3. ( 2 ) the chromosomes of bmn cells showed the typical characteristics of lepidoterati insects. chromosomes of matephase without apparent centromere are short pole - like and pellet - like

    （ 2 ） bmn細胞的染色體表現為典型的鱗翅目昆蟲的染色體：中期染色體短桿狀或顆粒狀、無明顯的著絲粒。
  4. The bmn cells are multiploidy and heteroploidy so highly that in which the diploid was few and far between, and multiploid was so much that came to 90 %. even there were 533 chromosomes in one cell

    Bmn細胞是高度異倍化、高度多倍化的細胞系，其典型二倍體極少而高多倍化群達90 ，最多的染色體一群在500條以上。
  5. ( 4 ) although the majority of bmn cells " mitoses are normal mitoses, there are still some abnormal phenomena found such as analogous amitoses, mitoses in multiploid cells and endomitoses

    K ） bmn細胞有絲分裂以正常有絲分裂為主，但同時存在多倍核有絲分裂、核內有絲分裂以及類似於縊縮和劈裂等的類無絲分裂等現象。
  6. The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent

    將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中，獲得重組轉移載體質粒pbacpak - hbmp 。
  7. Expression amount of rhbsag in the former was increased 60 % and 80 % respectively than the latter. antigenicity of pres2 of fusion protein was increased 8 times and 10 times in bmn cells and in pupa hemolymph respectively than nonfusion hbsag

    Bmpak - hbmp表達的融合m蛋白（ pres2 + s ）中， pres2抗原性顯著提高，與bmpak - hbm表達的非融合蛋白相比，融合蛋白pres2抗原性在細胞中約提高了8倍，蛹中約提高了1o倍。
  8. The reasults are summed up as following : ( 1 ) the more metaphase chromosomes of bmn cells and moderately contraction can be obtained by air - drying after cultured at the colchicine concentration 1. 0 u g / ml, 28 " c, for 6 - 9h

    用空氣乾燥法和培養細胞貼片法對bmn細胞的染色體和有絲分裂進行研究發現： （ 1 ）採用空氣乾燥法制備bmn細胞的染色體，在終濃度0 . 1ug / ml的秋水仙素28培養6 9h條件下可得到較多中期分裂相，染色體的凝集也比較適中。
  9. The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses

    同時，還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試，並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學，細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞，但其遺傳學和細胞生物學背景知之甚少。
  10. The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10

    重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
  11. This dissertaion, on the basis of the other studies, on the one hand, researched and analysed the chromosomes and mitoses of the cell line - bmn, which is widely used in the silkworm baculovirus expression system as an engineering cell. on the other hand, the dissertation attempted to explore establishment of the new silkworm embryoni cell line by primary culture

    同時，家蠶體外細胞培養研究的進行，不僅可以為家蠶細胞生物學的基礎理論研究提供良好的研究系統，而且在家蠶資源的開發與利用方面也具有重大的意義。本研究在借鑒前人研究的基礎上，一方面對現在廣泛應用於家蠶桿狀病毒表達系統的工程細胞? bmn細胞的染色體和有絲分裂進行了研究與分析。
  12. The expression activity reached the highest point at the 72nd hour in bmn cell ( 22 u / 2x ! 06cells ) and at 144th hour in larvae ( 159 u / ml ), respectively. elisa assay showed that expression product had angiostatin ' s immunoreactivity. western blotting assay also showed that product expressed in cultured cells was a 36 kd band, while product expressed in larvae of silkworms was two proteins, and the molecular weights were 37 kd and 42 kd, respectively

    表達產物活性在家蠶細胞中表達72小時達到最高值，經胞內表達產物作用后，血管內皮細胞的存活率僅為（ 28 . 0 3 . 0 ） ，表達量約為22u 2 10 ~ 6個細胞；在家蠶體內表達144小時活性達到最高值，內皮細胞的存活率僅為（ 6 . 4 0 . 5 ） ，表達量約159u ml 。
  13. Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf

    本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前，然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中，獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ，並與線性化bm - bacpak6dna共轉染家蠶細胞株，獲得重組病毒bacpak - 6aa - hgm - csf 。
  14. In the present thesis, it is mainly to express the human angiostatin ( kl - 3 ) in bombyx mori cells ( bmn ) and larvae of silkworms with baculovims expression vector system ( bevs ), to investigate the effect of human angiostatin ( kl - 3 ) on cell apoptosis in endothelial cells, and on vascular growth in chick embryo, and to elucidate cooperation between angiostatin and endostatin

    因此，在本實驗中我們用家蠶桿狀病毒表達系統表達了人血管抑素（ k1 - 3 ） ，並研究了其對內皮細胞凋亡和雞胚新生血管生長的影響，闡明了其與內皮抑素的協同作用。
  15. The bmn cells and fifth instars were infected by the recombinant virus bacpak - angiostatin. the bioactivity of the protein product was determined by human umbilical vein endothelial cells ( ecv304 ) growth assay in vitro and inhibition of vascular growth assay in cam in vivo. angiostatin showed significantly inhibitory effect on endothelial cells

    重組病毒以moi = 10感染家蠶細胞（ 2 10 ~ 6個細胞/ 5ml ）和家蠶5齡幼蟲，表達產物用體外培養的人臍靜脈血管內皮細胞（ ecv304 ）及體內雞胚尿囊膜（ cam ）新生血管實驗檢測其抑制活性： （一）血管抑素可明顯抑制體外培養的內皮細胞增殖。
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