bovine serum 中文意思是什麼

bovine serum 解釋
牛血清
  • bovine : adj. 1. 牛科的；牛的；牛一樣的。2. 魯鈍的。n. 牛科動物。
  • serum : n. （pl. serums, -ra ）1. 【醫學】血清。2. 血漿。3. 漿液；樹液。4. 乳清；乳漿。
  1. Interaction of norfloxacin with bovine serum albumin

    諾氟沙星與牛血清白蛋白結合作用研究
  2. Interaction of bovine serum albumin with benzoate

    光譜法研究苯甲酸鈉與牛血清白蛋白的作用
  3. The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells

    研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
  4. Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus, two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196. after 6 days cryopreservation, one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other, cryopreserved for 13 days, was 52

    以細胞培養液m199 （含20 %的小牛血清）為稀釋液， dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣，採取先慢后快的方式，冷凍保存兩組草魚囊胚晚期細胞於一1 %的液氮中。第一組冷凍保存6天後解凍，成活率為72 % ，第二組冷凍保存13天後解凍，成活率為520 / 0 。
  5. Was bound to bovine serum albumin to form a complete antigen e

    C3處的酚oh ，與牛血清白蛋白bsa偶聯，制備得e
  6. Part iv in this paper, 28 physiologically ph7. 43 ( + 0. 02 ), applying fluorescence method, we observed the fluorescece quenching of bovine serum albumin ( bsa ) by its interaction with cu ( ii ) and zn ( ii ) or cu ( ii ) absolutely. we calculated stern - volmer ' s - k ' svotbsa, and lineweaver - burk ' s a ^ ' dcu - bsa in these two kinds of systems. the different interaction of cu ( ii ) and zn ( ii ) with bsa has been discussed in this paper

    在出er卜lmcfap人公瓦的全懶上吐似推導了此三元體系中熒光碎滅的締合公式。在與二元體系的比較后，推測zno ）會竟爭cu門）與bsa結合， cu門川與bsa結合的穩定性大於zn ）與bsa結合的穩定性。
  7. Spectroscopy study on the interaction between colchicine and bovine serum albumin

    秋水仙堿與牛血清白蛋白相互作用的光譜性質研究
  8. The concentration of fbs ( fetal bovine serum ) and ncs ( newborn calf serum ) was influential on the culture process of mef ( mouse embryonic fibroblast ) and es cells

    血清濃度的高低對胎鼠成纖維細胞和es細胞的培養過程是有影響的。無血清或低濃度的血清能抑制細胞分裂增殖，使細胞生長出現停滯。
  9. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  10. The protein accounting for the total was approximately 11. 80 %. the peptide was synthesized according to the sequence of grb - ast7. since ast7 is a hapten, it was conjugated to the carrier proteins bovine serum albumin ( bsa ) using l - ethyl - 3 - ( dimethyl - aminopropyl ) carbodiimide ( edc )

    根據ast _ 7氨基酸序列合成小肽，用edc做偶聯劑把合成的半抗原小肽與載體bsa偶聯成完全抗原，免疫家兔三次后，采血收集抗血清，用elisa測定效價為1 400 。
  11. Preparation and determination of galactosyated bovine serum albumin

    半乳糖基牛血清白蛋白的制備和測定
  12. Ethyl violet - based spectrophotometric determination of bovine serum albumin

    乙基紫分光光度法測定牛血清白蛋白
  13. Fluorescence spectral study on interaction of tns with bovine serum albumin

    與牛血清白蛋白相互作用的熒光光譜研究
  14. Studies on the interaction between sulfadimethoxine and bovine serum albumin

    磺胺二甲氧嘧啶與牛血清白蛋白相互作用的研究
  15. Study on the interaction between tetrabromofluorescein and bovine serum albumins

    曙紅與牛血清白蛋白相互作用的熒光光譜研究
  16. Bovine serum albumin

    或牛血清白蛋白
  17. Anti - melatonin monoclonal antibodies of higher titer, affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections. we obtained 6 strains of hybridoma, all of them secreting specific antibodies to mt, we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique. an inhibition curve for mt was obtained in the range of 50pg to30ng, and 1. 4ng of mt inhibited the value of the assay by half. we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues, the moabs recognized mt but

    通過將mt用甲醛作連結劑連結到牛血清白蛋白上sa採用皮下多點注射兔疫小鼠得到了高效價，高親和力，較好特異性的抗mt單克隆抗體，最後獲得了5株單克隆細胞株，都能分泌針對mt的特異性抗體，建立了選擇性基團免疫分析法，用制備的抗體測定了人血清中mt的含量，作了mt的抑制標準曲線，其抑制范圍從50pg ? 30ng ，半抑制量為1
  18. In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks

    方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。
  19. Three dimensionally ( 3d ) - ordered macroporous materials of bovine serum albumin ( bsa ) and fluorescein isothiocyanate ( fitc ) labeled bsa were prepared by means of colloidal crystal as template

    摘要用膠體晶體為模板制備了牛血清白蛋白和異硫氰基熒光素標記的牛血清白蛋白的三維有序大孔材料。
  20. In this thesis the in situ brilliant cresyl blue ( bcb ) dimer with weak fluorescence in the solution of anionic surfactant ( as ) sodium lauryl sulfonate ( sls ) was used as fluorescence probe. at the same time, the new method for the determination of bovine serum albumin ( bsa ) was built. the linear range was 0 - 7mg / l and the detection limit ( 3 o / k ) was 3. 66 10 - 3 mg / l, the r. s. d for the determination of 2. 00mg / l bsa was 2. 77 %

    本論文以陽離子染料燦爛甲酚藍在陰離子表面活性劑十二烷基磺酸鈉存在下形成的現場弱熒光二聚體為熒光探針，建立了測定牛血清白蛋白的新方法，該法線性范圍為0 7mg / l ，檢測限為3 . 66 10 ~ （ - 3 ） mg / l ，對2 . 00mg / l幾的牛血清白蛋白平行測定8次，相對標準偏差為2 . 77 ，說明本法準確可靠。
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