brown staining 中文意思是什麼
brown staining
解釋
棕褐色變-
After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。 -
The orange - brown, scalloped appearance of these lesions is consistent with old contusions. the resolution left behind hemosiderin from the hemorrhage that produces the orange - brown staining
棕黃色圓齒狀外觀的病變與舊挫傷相一致。出血后血液溶解形成的含鐵血黃素產生棕黃色外觀。 -
Staining may vary from dog to dog and may be light pink or a rusty brown in color
而淚痕因狗爾異，可能是淺粉紅色或是銹褐色。 -
Results : the retroviral vector containing vegf gene was constructed successfully. immuohistochemistry showed that it expressed vegf protein in bmsc plasma ( brown staining ) and the experimental study confirmed that its mvc was higher than that in control group ( p < 0. 01 ). conclusion : gene transfer technology mediated by retroviral vector
結果:成功構建vegf逆轉錄病毒表達載體，免疫組化方法顯示經感染的bmsc胞質中有陽性棕色顆粒出現，且動物實驗顯示新生血管計數明顯高於對照組（ p < 0 . 01 ） 。 -
Escs can be induced into neuron - like cells by sequential neural induction the undifferentiated escs grew in colonies, with clear bound. alkaline phosphatase staining showed dark brown positive particles were distributed in every esc colony. after the sequential neural induction, the cells converted into neuron - like cells, with homogeneous forms, which had round bright cell bodies and thin long bipolar or multipolar processes
5誘導分化前後細胞端粒酶活性檢測（ trap ）結果1 .使用序貫誘導法可將escs大比例誘導分化為神經元樣細胞未分化的escs聚集成團狀生長，細胞排列緊密，集落邊界清楚，堿性磷酸酶染色呈強陽性，胞漿布滿棕黑色顆粒。
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