c gene segment 中文意思是什麼
c gene segment
解釋
c基因片段-
Arginine rich human histone h3 is a kind of basic nucleosome protein. in the medium of physiological condition, by electrostatic interaction, histone h3 which arginine impart to it a positive charge binds to dna which phosphate groups impart to it a negative charge. in this paper, in order to establish a new technology of transfection, the functional domains la and ii gene segments of pea were lined with histone h3 gene segment, then a recombinant fusion protein was constructed which serves as c arrier for transfer of dna via receptor - mediated endocytosis
本實驗在已成功獲得的pea功能區a ，功能區基因片段與人組蛋白h3基因片段的克隆菌株的基礎上，構建原核表達載體，將克隆質粒pmd - 18t - pea經nco ， mlu雙酶切，回收酶切產物；將克隆質粒pmd - 18t - h3經mlu ， xho雙酶切，回收酶切產物。 -
The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
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