calf serum 中文意思是什麼
calf serum
解釋
小牛血清-
Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus, two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196. after 6 days cryopreservation, one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other, cryopreserved for 13 days, was 52
以細胞培養液m199 （含20 %的小牛血清）為稀釋液， dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣，採取先慢后快的方式，冷凍保存兩組草魚囊胚晚期細胞於一1 %的液氮中。第一組冷凍保存6天後解凍，成活率為72 % ，第二組冷凍保存13天後解凍，成活率為520 / 0 。 -
The concentration of fbs ( fetal bovine serum ) and ncs ( newborn calf serum ) was influential on the culture process of mef ( mouse embryonic fibroblast ) and es cells
血清濃度的高低對胎鼠成纖維細胞和es細胞的培養過程是有影響的。無血清或低濃度的血清能抑制細胞分裂增殖，使細胞生長出現停滯。 -
But in experiment ii, the obtained result of measurement and examination is not better than control group 。 conclusion : mixed animo acids can be safely applyed to vessel cryopreservation instead of calf serum 。 the effect of the groups of the rapid freezing method is not better than the groups of the rate - controled slow freezing
而實驗ii的血管其內皮結構不清晰，內皮細胞超微結構變化較大。結論：復方氨基酸可代替胎牛血清用於血管的深低溫保存.速凍降溫方式對血管深低溫保存效果不及慢凍降溫方式。 -
Method : 120 rabbit vessels were randomly divided into three groups : the vessel of control group in cryopreservative medium containing calf serum were subjeted to rate - controled slow freezing ; those of experiment group i in cryopreservative medium containing mixed animo acids were frozen by the same method as in control group ; and those of experiment group ii in the same cryopreserative medium as in control group were subjected to rapid freezing
材料和方法：取中國純種家兔股動脈120段，隨機分為3組：對照組用含胎牛血清的保護液處理，以慢凍方式降溫；實驗組1用含復方氨基酸的保護液處理，以慢凍方式降溫；實驗組ii用含胎牛血清的保護液處理，用速凍方式降溫。 -
Various concentrations of rrta and rrta - yqrl were added into the wells and incubated for 6 h to allow internalization of toxins by fluid - phase endocytosis. the cells were then incubated in rpmi - 1640 medium containing 10 % fetal calf serum for another 24 h
結論本實驗用基因工程方法原核表達了rl 』 a蛋白和rl 』 a一yqrl蛋白，利用rta蛋白和f3ga的結合特性，溫和、簡單、有效地洗脫了目的蛋白。 -
In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
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