camv 中文意思是什麼

camv 解釋
virus]花椰菜花葉病毒
  1. Up to date, the cauliflower mosaic virus ( camv ) 35s promoter and its derivatives are used commonly. no report show transgenic plants expressed badh from halophyte and using its own promoter or other strong promoters

    目前在遺傳工程中所用的啟動子多為35s ，還沒有高效脅迫誘導啟動子的實際運用的報道。
  2. Agobacterium tumefaciens strain a311 carrying the plant tranfer vector pb1121, which contains the neomycin phosphotransferasell gene ( nptll ) and p - glucuronidase reporter gene ( gus ) both under the control of the camv 35s promoter, was used in the establishment of the genetic tranformation of white clover

    選用苗齡4 5天的帶柄子葉作為外植體，先將外植體預培2天，再與根癌農桿菌a311共培養3 4天後，轉入附加有40mg l ~ （ - 1 ）卡那黴素和400mg
  3. Diagnosis and genetic analysis of resistance to cauliflower mosaic virus in brassica crops which was transformed with camv gene vi

    蕓薹屬蔬菜植株抗病性鑒定及其遺傳分析
  4. And then the ced - 9 gene was inserted into plant expression vector pbi121 under the control of camv 35s promoter

    在此基礎上再構建重組表達載體pbi - ced9 ，將ced - 9基因置於camv35s啟動子控制之下。
  5. We confirmed that the cloned gene dreb1c was really transformed into arabidopsis by pcr method, in which sequences of camv 35s were used as forward primer

    提取轉基因植株的基因組dna ，進行pcr反應，證明了dreb1c確實轉入擬南芥中。
  6. 2. in order to express chs in plant, we constructed plant expression vector p1301b q c. first added camv 35s promoter and nos terminator to chs by one median clone

    （ 2 ）為了能在在植物中表達（ chs ，通過一步的中間克隆，將chs連上camv35s啟動子和nos終止子。
  7. This gene, artificially put under the control of camv 35s, was introduced into tobacco with the aid of agrobacterium, and the transgenic plants obtained were identified by gus staining, pcr and southern blot analysis

    將arge與組成型啟動子camv35s相連，構建植物表達載體，通過農桿菌介導轉化煙草。經過gus染色、 pcr及southern鑒定獲得了轉基因植株。
  8. By blue - white screening and sequence analysis, we obtained the positive clone. 2. we constructed plant binary expression vector carrying fusions of the enhanced camv 35s promoter and dreb1c ( 35s : dreb1c ) in the sense orientation

    我們從擬南芥基因組中克隆了dreb1c基因全長，並將其連接到中間載體pgem - t - easy上，進行了測序驗證，結果顯示所克隆的dreb1c序列完全正確。
  9. To target this mitochondrial enzyme into chloroplast, the cdna sequence of mnsod was fused to a chloroplast transit peptide from a pea rubisco small subunit gene, whereas expression of the chimeric gene was driven by the camv 35s promoter

    Pchlsod質粒含有煙草mnsod基因的cdna序列，與豌豆核糖體小亞基葉綠體引物肽（ tp ）的編碼基因序列構成融合基因，由35s啟動子調控。 npt基因為選擇標記基因， pgv2260為輔助質粒。
  10. Driven by the cauliflower mosaic virus ( camv ) 35s promoter, the er - shsp over - expressed constitutively. the growth and the phenotype of transgenic plants can be used for researching the function of er - shsp in improving tomato ' s cold resistance and the er - shsp chaperones function in vivo. after degested by kpnl and xbal enzymes from the pbs - er plasmid, the gene er - shsp - lehsp21. 3 was inserted into the prokii vector to construct an eukaryotic expressing vector

    利用基因工程方法，將內質網小分子熱激蛋白基因（ endoplasmicreticulum - locatedsmallheatshockproteingene ， er - shspgene ） - lehsp21 . 3導入到番茄體內，使之在植物體中組成性表達（ constitutiveexpression ）內質網小分子熱激蛋白（ er - shsp ） ，觀察轉基因番茄在低溫條件下的生長和表型反映，研究er - shsp在提高植物耐寒性中的作用，同時為體內研究er - shsp的分子伴侶機制提供依據。
  11. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。
  12. In this construct, hf2 dna should be expressed under the control of the camv 35s promoter. the construct was transformed to petunia hybrida of the light pink via agrobacterium tumefaciens eh a105 using the leaf disc method. in the end, three transgenetic plants were obtained by screening with the phosphinothricin resistance and pcr amplification

    通過三親交配將重組質粒pc3301 - hf2導入根癌農桿菌eha105 ，抗性篩選、 pcr檢測及dna點雜交表明轉化后的農桿菌帶有完整目的基因片段，能夠用於轉化植物。
  13. In this dissertation, influences of various factors on plant regeneration and agrobacterium - mediated transformation of tall fescue embryogenic calli were systematically studied, and thereafter, stress tolerance - related cbf1 gene guided by constituent promoter camv 35s was incorporated into genome of this grass to obtain transgenic plants with increased stress tolerances

    本論文在對高羊茅胚性愈傷組織植株再生與農桿菌介導遺傳轉化的多種影響因素進行系統研究的基礎上，將組成型表達啟動子camv35s引導的耐逆相關cbf1基因導入該草種的基因組，獲得耐逆性增強的轉基因植株。
  14. The plant expression vector pbin19 - rok219 was constructed, which contains cauliflower mosaic virus ( camv ) 35s promoter and noster. introduction of ibdv vp2 gene under control of camv 35s promoter resulted in the construction of binary expression vector pbr - vp2. then the vp2 gene was in the left and right border regions, which denote the limits of the dna that is integrated into the plant genomic dna via agrobacterium fwme / ac / ms - mediated transformation

    構建了表達載體pbin19 - rok219 ，以ibdv甘肅天水株的抗原基因vp2為目的基因，將vp2基因置於植物組成型表達啟動子camv35s之下，構建了一個ibdvvp2基因的植物表達載體pbr - vp2 ，這樣使vp2基因位於農桿菌t - dna的左右邊界重復序列之間。
  15. Three binary expression vectors were constructed, which harbored the cl - i - 2kl, r - 2kl, and d - l - 2kl - r - 2kl respectively, driven by the camv 35s promoter. then, agrofeac / erium - mediated transformation of iobacco ( nicotiana tabacum, w38 ) was carried out. transformed tobacco lines were obtained through tissue culture and confirmed by pcr and southern blotting analysis

    通過分子克隆技術，將c1 - i - 2k1 、 r - 2k1和二者的連接（ c1 - i - 2k1 ? r - 2k1 ）分別構建到雙元表達載體上，並使它們分別置於35s啟動子的驅動之下，然後通過農桿菌介導的技術分別對煙草（ nicotianatabacum ， w38 ）進行了轉化，利用組織培養技術再生植株。
  16. We excised the pgn fragment, which contains a camv 35s promoter a gus gene and a nos terminator from the plant expression vector pbi121 with ecor i and hindlll and inserted it between the ecor i and hindlll sites of the plant expression vector pcambia3301, yielding pc3301 - pgn. the hf2 fragment was excised from pg - ho with xba i and sac i and then cloned between the xba i and sac i sites of the plant expression vector pc3301 - pgn to produce pc3301 - hf2

    將pbi121植物表達載體上含有camv35spromoter 、 gusgene和nosterminator的pgn片段酶切下來，連接到植物表達載體pcambia3301上，構建成中間載體pc3301 - pgn ，然後用hf2dna片段替換pc3301 - pgn載體上的gus基因，構建成植物表達載體pc3301 - hf2 ， hf2dna正向插入在植物表達載體35s啟動子的下游。
  17. In this study, a pcr detection method on camv 35s promoter and nos terminator which are extensively used in gmc as regulatory elements. the pcr detection result showed : the practical detection limit was 0. 5 % ( w / w ), which meant this method could detect the gm soybean when its weight proportion was equal or exceeded 0. 5 %

    本研究初步建立了轉基因農作物常用的調控基因35s啟動子和nos終止子的pcr檢測技術，檢測結果表明：本實驗的轉基因檢測下限為0 . 5 （ w w ） ，即當受檢材料中的轉基因成分佔非轉基因成分等於或大於0 . 5時可以檢出。
分享友人
分享到 Line

分享到臉書