cell fractionation 中文意思是什麼

cell fractionation 解釋
細胞[組分的]分級分離
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  • fractionation : n. 【化學】分餾（法）；分層分離，分級分離，份化。
  1. The second general problem of cell fractionation is the contamination of each fraction by others.

    細胞組分分離的第二個普遍問題是各組分的相互摻雜。
  2. - acetolactate decarboxylase is purifed from cell extract by 50 % - 80 % ammonium sulfate - fractionation, 50, 2min heat treatment and deae - sepharose fast flow column chromatography, which we study the different ph and different buffer of deae - sepharose fast flow column chromatography and conclude ph 6

    對其酶學性質進行了研究。 -乙酰乳酸脫羧酶經50 80硫酸銨分級沉澱、 50 ， 2min熱處理、 deae - sepharosefastflow離子交換柱層析方法分離純化。
  3. - acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms. the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product. in this paper, - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation, heat treatment, deae - sepharose fast flow column chromatography

    本文對來源於枯草芽孢桿菌（ bacillussubtilis ） 3226 - 5的-乙酰乳酸脫羧酶經硫酸銨分級沉澱、熱處理、 deae - sepharosefastflow離子交換柱層析等分離純化步驟，得到sds - paeg電泳純，通過n末端氨基酸序列分析驗證酶蛋白的純度。
  4. The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100. the sod activity for purified enzyme could reach 4966 iu / mg proteins, and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis. when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0. 2mmol / l kcn or 0. 5 mmol / l h2o2, the active band was still showed at the same position, which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod

    通過sds一聚丙烯酸胺凝膠電泳可知該sod酶的分子量約為20kda .在非變性聚丙烯酞胺凝膠（ pagb ）電泳后，在凝膠son顯色反應液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝膠sod顯色反應后在原來的sod酶部位仍然含有sod活性帶，這表明利用kcn和h20 :處理並不能抑制500的活性，該sod屬于mn一sod 。
  5. Subcellular localization by cell fractionation, immunofluorescence revealed that three human isoforms were associated with membranes involved in the autophagic pathway. the glycine and lysine respectively decided the location of autophagosome of human map1lc3a and map1lc3b. in addition, our results indicated that other proteins were involved in the post tranlational modification of human map1lc3b and this modication could be induced by amino acid starvation

    人lc3a的膜定位與gly120位點的切割和修飾緊密相關，而人lc3b的膜定位是與lys122相關，進一步證明了修飾方式的特異性，對人lc3b的進一步研究表明參與其修飾的分子也與其它基因不同，並且饑餓可以誘導其修飾的發生。
  6. New application of a subcellular fractionation method to kidney and testis for the determination of conjugated linoleic acid in selected cell organelles of healthy and cancerous human tissues

    結果發現各細胞器間存在交叉污染，是一個亞細胞研究的一個反例。
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