chain blast 中文意思是什麼
chain blast
解釋
連鎖沖擊-
A pair of degenerate primers were designed in the conserved domain which based on the alignment of acbf gene family in tobacco and arabidopsis. a 239 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ), which was used as a probe for screening tomato fruit ( pink stage ) cdna library. one positive clone containing entire coding region were isolated, which was identified as a new member of acbf family by blast server, and named as leacbf
根據genbank (中的擬南芥、煙草acbf家族成員序列比較的結果,在該基因的保守區設計簡並引物( degenerateprimer ) ,以轉色期普通番茄果實的rna為模板,進行rt - pcr擴增,獲得239bp的擴增片段,以此片段作為探針篩選轉色期普通番茄果實cdna噬菌體文庫,獲得了包含全長編碼區的陽性克隆。 -
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