chimeric vector 中文意思是什麼
chimeric vector
解釋
嵌合載體-
Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into
經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。 -
Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato
核酶嵌合基因植物表達載體的構建及對番茄的轉化 -
Finally, the chimeric gene was inserted into binary ti vector plbj21, and cloned into agrobacterium tumefaciensgv3101 by electroporation
為了提高can小肽表達強度，本實驗構建了can小肽二連體。 -
Megf and melittin was linked together by a linker into an chimeric dna, megfmel, and the chimeric dna was expressed by using pet30a as expression vector and e. coli as expression strain
值在slug砌l與54ug加l之間。在誘導表達各目的產物時誘導溫度為30c 。 -
They indicated that ecbp21 may have some relative with the response to stress treatments. further more, we constructed the binary vector containing ecbp21 - gfp chimeric gene and got transgenic plants by using the agrobacterium vacuum infiltration method
4 .初步開展了轉基因研究:為了進一步確定ecbp21在植物生長發育過程中的作用，構建了ecbp21植物表達二元載體，通過真空滲透法轉化了擬南芥並獲得了轉基因植株。 -
The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。 -
Furthermore, by inserting " anther box " element to the mutated area of two site - mutation promoters, another two promoters, ipmas and ipmal, were created. in order to study the chemical - inducible capacity of wild and modified pr - la promoters, a coding sequence of gus ( | 3 - glucuronidase ) gene was fused to their downstre am, and the chimeric genes were cloned into pbin ! 9 - based plant expression vector
為了檢測得到的啟動子驅動效率及誘導活性，將所得到的啟動子、定點突變啟動子和插入花藥盒的啟動子與gus基因連接，構建了6個植物表達載體，同時分別構建包含ipl barnase 、 ipml barnase 、 ipmal barnase嵌合基因的植物表達載體。
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