cloning by expression 中文意思是什麼

cloning by expression 解釋
表達克隆
  • cloning : 基因轉殖 克隆
  • by : adv 1 在側，在旁，在附近。2 （擱）在一邊，（放）到旁邊，（存）在一旁；收著。3 （由旁邊）經過，過...
  • expression : n 1 表現，表示，表達。2 詞句；語句，措辭，說法。3 表情，臉色，態度；腔調，聲調。4 【數學】式，符...
  1. Cloning and expression in e. coli of lactase. specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis. klac gene was amplified by pcr and subsequently cloned

    乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母（ kluyveromyceslactis ）菌株k538的基因組dna為模板，設計引物，利用pcr獲得乳糖酶基因klac ，經dna測序驗證，得到克隆t1549 。
  2. One was cloning, sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ), the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot

    為了鑒別診斷tgev與prcv及對tgev進行流行病學調查，本研究採用原核表達系統（ gst融合表達系統）表達tgev纖突蛋白（ s蛋白）中含有b和c抗原位點的多肽，並且制備了非放射性地高辛標記的核酸探針，通過斑點雜交（ dot - blot ）檢測tgev核酸rna 。
  3. In our study we have cloned the osd gene from s. typhimurium by pcr, characterized the gene product and used this gene to construct asd + expression cloning vectors ptrc99a - asd

    再將hpylori尿素酶b亞單位基因與尿素酶b和熱休克蛋白a融合基因分別克隆入ptrc99a一asd質粒的多克隆位點之內。
  4. 2. cloning and functional expression of a heterogenous gene from a. nidulans in e. coli strain a. nidulans chromosome dna was used as template to amplfy gene qutb by pcr, the dna sequencing showed that the cloned gene was in agreement with the reported sequence

    異源奎尼酸脫氫酶（ qdhase ）在大腸桿菌中功能性表達以構巢麴黴菌（ a . nidulans ）基因組dna為模板，用pcr方法擴增得到了qutb基因，序列測定與文獻報道一致。
  5. Gene cloning differential fragment gha27 was produced by cdna - aflp method employed to compare the gene expression in developing anthers between the male sterile and fertile plants of cotton dong a. a cdna clone, designated gharf ( gossypium hirsutum adp - ribosy lation factor ), was isolated from a cotton ( gossypium hirsutum l. ) cdna library using gha27 as probe. 2

    棉花arf基因的克隆選取棉花洞a雄性不育株和可育株花藥cdna - aflp差別顯示的差異片段gha27 ，用地高辛標記作為探針，篩選棉花洞a花藥的cdna文庫，克隆得到了棉花arf基因的cdna全長序列，將其命名為gharf （ gossypiumhirsutumadp - ribosylationfactor ） 。
  6. In the first part, the focus is to find the receptor molecules directly by screening two cdna libraries with a recombinant construct prpap or as an alternative, to find an enriched area in the embryo brains and construct libraries from this brain region and perform the expression cloning as above

    方法： （ ）以融合蛋白prpap通過瞬時表達克隆法篩選兩個cdna文庫，或者通過與胚胎腦的結合實驗篩選有豐富結合蛋白的腦區，以圖構建cdna文庫並進行表達克隆的篩選。
  7. Two types of repeat sequence, a 15 - amino - acid ( eelcaqlcstppppi ) with 2 repeats and a 6 - amino - acid ( ppictp ) with 4 repeats, were firstly reported. 2. the characterization of meq gene product and its expression within the cells a recombinant baculovirus transfer vector pblubac4 - meq was constructed by cloning meq gene of marek ' s disease virus ( mdv ) ga strain into the baculovirus transfer vector pbluebac4 under the polyhedrin promoter

    此外，研究還發現了meq基因的兩類有趣的重復結構，其中一類是含15個氨基酸殘基（ eelcaqlcstppppi ）的結構，有2個重復，另一類是含6個氨基酸殘基（ ppictp ）的結構，共有4個重復，它們全部分佈在meq蛋白c -端的轉錄激活域內。
  8. The full - length sequence, the 3 ' - deletion fragment, the sequence encoding the mature protein and the sequence encoding the conservative domain, were cloned using synthesized primers. recombinant expression vectors were constructed through directional cloning and then host e. coli were transformed by the vectors

    設計特異引物克隆得到毒蛋白基因的4個片段，即基因全長、末端缺失片段、編碼成熟肽的片段及編碼活性區域的片段。
  9. A fusion - trascriptional library was then constructed by inserting sau3ai - partially digested chromosomal dna from 7653r into bamhi cloning site of phn127. a group of constitutive - expression fusions with different fluorescent strength were obtained

    將7653r的總dna經sau3ai部分酶解並克隆到phn127上，獲得的融合子庫，從中篩選到gfp組成型表達的具有調控活性的dna片段。
  10. In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells

    為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。
  11. Pcr products were inserted into pbv - 220 with double digestion of restriction enduonuclease. the expression vectors of pbv - a pbv - b and pbv - c were constructed by orientaional cloning. through sds - page, bioactivity and function analysis of expressed protein, the function of phaa, phab and phac was confirmed

    菌株的亞克隆基因組片段中，分離出phaa 、 phab和phac三個基因片段，定向克隆至原核表達載體pbv220上，構建了三個原核生物表達載體pbv - a 、 pbv - b和pbv - c ，通過對表達載體誘導表達，表達蛋白產物的sds - page分析、生物活性與功能分析，確定了基因phaa 、 phab和phac的生物學功能。
  12. Phaa, phab and phac were inserted to pbv - 220 with double digest of restriction enduonuclease. the expression vectors of pbv - a, pbv - b and pbv - c were constructed by orientaional cloning. indefication of expression vector with restriction enduonuclease digest showed that phaa phab and phac were in right orfs

    將phaa 、 phab和phac片段雙酶切后，定向克隆至原核表達載體pbv220 ，構建了三個原核表達載體pbv - a 、 pbv - b和pbv - c ；經酶切分析表明，所克隆的三個基因phaa 、 phab和phac置於表達載體的正確閱讀框架下。
  13. 1. expression of cry genes located in native plasmid in different flagella serotype strains to study cloning and expression of icps genes, an ecor i - f fragment of cryla ( a ) gene from pesi was inserted into pselect - 1 with t7 rna polymerase promoter in vitro. the plasmids of bacillus thuring fensisybt - 803 and ybt - 791 were analyzed by southern hybridization using an rna probe of ecori - f fragment and by pcr identification with cryl mixture primers

    將cry基因的高保守區的cry a （ a ） ecog - f片段插入帶有t7rna聚合酶啟動子的質粒pselect - 1 ，獲得了能在體外轉錄的rna探針載體pbpl - 1 ，用該載體制備的rna探針具有特異強，背景清楚，省時省力等優點，已成功地用於蘇雲金芽胞桿菌的分子生物學研究和特異菌株的篩選。
  14. Presently, the scientist have made out some successes by using biotechnology and molecuology, for example, planting out regeneration shoots, culture suspended cell in ferment tank, screening mutant tissue with high content of flavonoids, cloning and sup - expression of key enzyme, restraining derivative pathway by anti - dna or anti - rna and inducing genetic transformated hairy root

    通過培養水母雪蓮的發狀根將是獲得雪蓮類黃酮極有前途的方法，目前還是一項待填補的空白。發根農桿菌（ agrobacteriumrhizogenes ）是一種革蘭氏陰性土壤細菌，細胞內有200kb左右的雙鏈閉環dna ? ri質粒（ rootinducingplasmid ） 。
  15. The interest gene was inserted in the - tha l. tho 1 multiple cloning sites of donor plasimd pfastbachtb of baculovirus expression system. after analysis by restriction endonuclease and pcr, the recombinant donor plasmid gpl - fast was transforn1ed to the competent celi dhi0bac which contalns the bacmid and the helper plasmid, the recombinant bacmid gpl - bac was acquired which would express the vpl of gpv strain h l

    同時將該目的基因插入到桿狀病毒表達系統的供體質粒pf _ （ ast ） b _ （ ac ） htb的xba 、 xho多克隆位點間，經酶切、 pcr鑒定后，將重組的供體質粒gp1 - fast轉化到含有桿狀病毒和輔助質粒的dh10b _ （ ac ）感受態細胞中，獲得了表達gpvh1株vp1的重組桿狀病毒gp1 - bac 。
  16. On the base of construction of pbi121vp7, we constructed the fusion gene expression vector pbi121ctbvp7 by the same sigle cloning site ( ndei and xbai ) of vector puc19ctb and pbi121vp7

    設計具有ndei和saci單限制性酶切位點的vp7基因引物，通過pcr擴增出vp7基因並經測序驗證。
  17. Sequence analysis shows that they share 98. 75 % similarity at the dna, and 98. 67 % at the protein level. ns2 gene was cloned into the multiple cloning sites of prokaryotic expression vector pgex - 6p - l. the recombinant plasmid pgex - 6p - ns2 was constructed and transformed to the competent cell bl21 ( de3 ) plyss, positive bacterium strain was induced by iptg

    將ns2基因插入到原核表達性質粒pgex - 6p - 1的ecor 、 bamh多克隆位點之間，將重組原核表達質粒pgex - 6p - ns2轉化到bl21 （ de3 ） plyss感受態細胞中，獲得了表達ns2基因的陽性亞克隆重組子，在含amp的lb液體培養基中培養，經iptg誘導表達，用sds - page分析表達產物。
  18. Construction of rab5a gene eukaryotic expression vector by direct cloning

    1基因的克隆及其在成骨細胞中的表達
  19. Cloning and expression of human soluble tumor necrosis factor receptor by e. coli and appraisement of its activity

    基因多態性與非酒精性脂肪性肝病胰島素抵抗的相關性
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