cloning dna 中文意思是什麼

cloning dna 解釋
克隆dna
  • cloning : 基因轉殖 克隆
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  1. Gene cloning provides a means of purifying and propagating specific dna segments.

    基因克隆化提供了一種純化和擴增特定DNA片段的方法。
  2. Cloning of ctsox2 gene shows the validation of rpgw - pcr used for isolating genes from the digested genomic dna

    C心以2基因的hmg盒區外的序列信息可以進一步研究它的功育旨。
  3. To test the p7 promoter activity, a series of constructs were obtained by cloning the different dna fragments into the luciferase gene reporter vector pgl3 - b. when the constructs were transiently transfected into thp - 1 cells, luciferase activity assay showed that the core region of p7 promoter located at - - 165 bp

    第一部分的工作首先通過對人acat - 1基因p7啟動子序列進行5 '和3 ' -端的缺失的詳細分析,結果顯示最大報告基因轉錄表達活性位於- 612 - 165bp區段。
  4. Recombinant dna techniques deeloped for cloning genes

    重組dna技術被應用於基因克隆
  5. Recombinant dna techniques developed for cloning genes

    重組dna技術被應用於基因克隆
  6. Recent studies show that dna transfer and recombination technology provides great potential for genetic improvement of zoysiagrasses ; molecular linkage maps were constructed from zoysia japonica and its hybrids with zoysia matrella ; the researches on gene cloning and gene resource are in progress

    最近的研究表明,基因轉移與重組技術在結縷草遺傳改良中具有巨大應用潛力;結縷草遺傳連鎖圖譜構建取得重要進展;基因克隆和基因資源研究正在展開。
  7. Based on the previous studies, the research in this paper was carried out, mainly including two parts as follows : ( 1 ) anammox bacteria and aerobic ammonia oxidizers were detected in situ in 6 sediment samples taken from jiangsu province. molecular techniques, such as fish, pcr, dna cloning and sequencing etc. were used for this purpose. ( 2 ) the continuous cultivation of anammox bacteria from sediment samples were studied, which provides experimental basis for the bioaugamentation of eutrophicated sediment applying anammox process

    本論文在前人研究的基礎上,開展了以下兩個方面的工作: ( 1 )採用分子生物學技術熒光原位雜交( fish ) 、多聚酶鏈式反應( pcr ) 、 dna克隆和測序等對采自江蘇省蘇州市、東太湖、新沂河等6個底質樣品進行了厭氧氨氧化菌和傳統氨氧化菌的原位檢測; ( 2 )探討了以底質作為接種體進行厭氧氨氧化菌富集培養的可行性,為天然底質環境中厭氧氨氧化過程的強化,富營養化底質微生物修復的可行性提供一定的依據。
  8. In this sequel, john hammond richard attenborough summons chaos theorist and onetime colleague ian malcolm jeff goldblum to his home with some startling information - while nearly everything at his jurassic park had been destroyed, engineers were also operating a second site, where other dinosaurs, resurrected through dna cloning technology, had been kept in hiding

    創造了恐以後,富翁約翰,韓文博士成立了遺傳科技公司,而且將恐基因帶到侏羅紀公園附近一個荒島梭拿島lsla sorne繁殖其後代。一場颶風將島上儀器變成廢虛,在天然環境下恐的數目瞬速增加。
  9. The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent

    將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中,獲得重組轉移載體質粒pbacpak - hbmp 。
  10. In this method, high fidelity dna polymerase ( pyrobesf * dna polymerase ) was used to ensure efficient and accurate amplification. after cloning and sequencing of the pcr products, one concatemer contains seventeen copies of grb ~ ast7 was attained. the grb - ast ? concatemer was cloned into pet22b expression vector

    先用t4dnapolymerase構建成模板,再通過高保真dna聚合酶( pyrobesttmdnapolymerase ) pcr進行擴增,從中得到一個含有17個拷貝的grb - ast _ 7的串聯體。
  11. Cloning and sequence analysis of nosema bombycis s special dna fragment

    片段的克隆與序列分析
  12. Cloning and polymorphism analysis of rab1 genes from micronuclear dna of euplotes octocarinatus

    1家族基因的分離及多態性分析
  13. The a - complementation reaction of & - galactosidase ea and ed was also used in dna cloning, protein protein interactions monitoring and expressing immunoassays studying

    -半乳糖苷酶的ea 、 ed這種-互補性還被用於分子生物學、蛋白質相互作用的監控、表達免疫分析等方面的研究。
  14. Even animal clones that look healthy may have genetic abnormalities or be predisposed to a decreased life span because the cells used in the cloning process acquire dna damage as they age

    即使看起來很健康的克隆生物也會攜帶畸形病變的基因,而且他們的壽命通常會比正常生物的要短,因為克隆過程中的細胞隨著年齡的增長, dna會逐漸遭到破壞。
  15. Cloning of the glyphosate n - acetyltransferase gene from soil total dna and its characterization of enzyme activity

    乙酰轉移酶基因的克隆及酶學特性分析
  16. Firstly, the hyaluronate synthase ( has ) gene ( hasa ) was cloned into the cloning vector puc19 by pcr using the total dna sample of s. equi as template

    本文研究代謝工程理論,對本實驗室的一株ha生產菌( streptococcusequi )進行了ha合成與分解關鍵酶基因及功能的研究。
  17. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后,轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌,進一步用遺傳毒素g418篩選多拷貝的轉化菌株,命名為gh1 ;將gh1甲醇酵母菌用0 . 5的甲醇誘導表達,發酵上清經90飽和度的( nh _ 4 ) _ 2so _ 4沉澱, yw - 3 ( mwc03000 )的超濾膜超濾,再經cm陽離子交換, c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分,其中4289 . 05的組分經質譜鑒定,氨基酸組成分析和序列測定為正確的表達產物,生物學活性表明其活性為天然毒素活性70 % ,表達量為80mg / l 。
  18. Arc1, thl1 and thl2, the substrate protein genes of s receptor kinase, were cloned through a series of methods of molecular biology such as pcr, rt - pcr, dna cloning and sequencing. the resultings sequences were highly analysed by using the related biosoftwares on internet, providing new insights in the field of the molecular mechanism of self - incompatibility in plants. the major results are as followings : 1

    本文通過pcr和rt - pcr等一系列分子生物學方法克隆了蕓薹屬植物中的甘藍和油菜自交不親和信號傳導過程中srk底物蛋白基因arc1 、 thl1和thl2 ,並使用各種相關生物信息學軟體對srk底物蛋白基因序列進行了分析,然後在internet網上利用在線軟體對蛋白質的結構和功能進行了預測和探討,以期為蕓薹屬植物自交不親和性的分子機理的研究提供新的內容。
  19. At last, ctab - dna and sds - dna methods are used in this experiment. on the basis of optimizing experiment procedure and pcr system of the materials, the initial reversion transcription system and orthologus gene cloning technique are established

    對這3種方法比較后,確定本實驗採用ctab - dna酶消化法和sds - dna酶消化法提取白樺雄花芽組織的總rna 。
  20. Streptomyces low copy number plasmid scp2 * is also employed for the cloning and recovery of large dna fragment from streptomyces. as scp2 * can accommodate large dna insertions and is efficiently transmissible among its permissive hosts such as s. lividansbut seemed to be unable to replicates in streptomyces sp. fr - 008, it might be a suitable vector for the cloning of the entire fr - 008 pks gene cluster through gene replacement followed by conjugation with 5

    Scp2 *可以在變鉛青鏈黴菌等許可宿主內復制並在許可宿主間有效轉移,但似乎不能夠在鏈黴菌fr - 008中復制,因此可能適合於在鏈黴菌fr - 008中通過進行基因置換及隨后的鏈黴菌fr - 008和變鉛青鏈黴菌間的接合過程來克隆完整的fr - 008生物合成基因簇。
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