cloning site 中文意思是什麼
cloning site
解釋
插入位點,克隆位點-
In this sequel, john hammond richard attenborough summons chaos theorist and onetime colleague ian malcolm jeff goldblum to his home with some startling information - while nearly everything at his jurassic park had been destroyed, engineers were also operating a second site, where other dinosaurs, resurrected through dna cloning technology, had been kept in hiding
創造了恐以後，富翁約翰，韓文博士成立了遺傳科技公司，而且將恐基因帶到侏羅紀公園附近一個荒島梭拿島lsla sorne繁殖其後代。一場颶風將島上儀器變成廢虛，在天然環境下恐的數目瞬速增加。 -
The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中，獲得重組轉移載體質粒pbacpak - hbmp 。 -
The development of gene targeting and animal cloning thechnology makes the site - specific integration of foreign gene become possible
隨著基因打靶技術和動物克隆技術技術的發展，外源基因在高等哺乳動物基因組中的定點整合成為了可能。 -
A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally
進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。 -
A fusion - trascriptional library was then constructed by inserting sau3ai - partially digested chromosomal dna from 7653r into bamhi cloning site of phn127. a group of constitutive - expression fusions with different fluorescent strength were obtained
將7653r的總dna經sau3ai部分酶解並克隆到phn127上，獲得的融合子庫，從中篩選到gfp組成型表達的具有調控活性的dna片段。 -
According to csfv ' s e2 gene sequence published in genbank and the sequence of p. pastoris expression vector ppic9 ' s multiple cloning site ( mcs ), a pair of primers were designed with oligo and primers. o softwares
將該基因片段先克隆到pmd18 - t載體上，並進行了酶切、 pcr鑒定和測序分析，陽性重組質粒命名為t - e2 。 -
On the base of construction of pbi121vp7, we constructed the fusion gene expression vector pbi121ctbvp7 by the same sigle cloning site ( ndei and xbai ) of vector puc19ctb and pbi121vp7
設計具有ndei和saci單限制性酶切位點的vp7基因引物，通過pcr擴增出vp7基因並經測序驗證。 -
The ubi - sl - tocs fragment was taken out, inserted into the multi - cloning site of pcambia1300 vector, transformed into jm109 strain finally, positive colonies were screened on lb plate ( 60 g / ml kan added ). the result of pcr and enzyme digestion of plasmid proved that recombinatin vector was obtained ( named pcusaib4 and pcusaibu )
把擴增產物分別通過clai和bamhi酶切純化，連接到用clai和bamhi切去gfpml基因的中間表達載體pugfpocs中，轉化大腸桿菌jm109 ，在含amp （ 100 g / ml ）的lb抗性平板上篩選到了的陽性菌落。 -
And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8
試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。
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