cloning vector 中文意思是什麼
cloning vector
解釋
（克隆載體）：攜帶插入外源片段的質粒或噬菌體，從而產生更多物質或蛋白質產物。-
These are inserted into a cloning vector.
它們被插入克隆載體。 -
Rlean meat percentage is one of the most important economic traints in pig breeding programs. myostatin is a negative regulator of skeletal muscle growth. null or low activity of myostatin, individual muscle of mutant amimals would show a large and widespread increase in skeletal mass. myostatin null animals have significantly larger diameter or more quantity of fiber skeletal muscle. the phenotype was termed double muscling. in order to probe the relation between myostatin and high lean meat rate and plump - hipped trait, we sythesized the c ' 80 amino acids coding sequence of porcine myostatin and costructed the cloning and expressing vector of it
肌生成抑制素（ myostatin ，即mstn ）是近幾年來（ mcpherrona . c等， 1997 ）發現的骨骼肌生長的負調控因子，它主要在骨骼肌中表達。其活性的喪失，會引起動物肌肉的過度發育，肌纖維直徑變大或肌纖維數增加，表現為雙肌癥狀。肌生成抑制素研究的突破將對豬、肉雞、肉牛等畜禽生產性能的提高具有特別重要的意義。 -
Then the pcr product was purified, ligated into pgem - t vector by ta cloning
篩選陽性克隆，測序，大量制備序列完全正確的質粒。 -
To test the p7 promoter activity, a series of constructs were obtained by cloning the different dna fragments into the luciferase gene reporter vector pgl3 - b. when the constructs were transiently transfected into thp - 1 cells, luciferase activity assay showed that the core region of p7 promoter located at - - 165 bp
第一部分的工作首先通過對人acat - 1基因p7啟動子序列進行5 '和3 ' -端的缺失的詳細分析，結果顯示最大報告基因轉錄表達活性位於- 612 - 165bp區段。 -
Cloning of monomial promoter of actin1 gene and construction of plant expression vector
1的克隆及植物表達載體的構建 -
Cloning and construction of prokaryotic expression vector of vp1 gene of foot - and - mouth disease virus serotype o
1基因的克隆及原核表達載體構建 -
The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中，獲得重組轉移載體質粒pbacpak - hbmp 。 -
In this method, high fidelity dna polymerase ( pyrobesf * dna polymerase ) was used to ensure efficient and accurate amplification. after cloning and sequencing of the pcr products, one concatemer contains seventeen copies of grb ~ ast7 was attained. the grb - ast ? concatemer was cloned into pet22b expression vector
先用t4dnapolymerase構建成模板，再通過高保真dna聚合酶（ pyrobesttmdnapolymerase ） pcr進行擴增，從中得到一個含有17個拷貝的grb - ast _ 7的串聯體。 -
Since the plasmid is capable of independent replication in host cells of many dicotyledonous plants, it has been used as a cloning vector in gnetic engineering
在許多雙子葉植物中質粒在寄主細胞中是獨立的復制單元，所以可以在基因工程中用作克隆載體。 -
Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。 -
Firstly, the hyaluronate synthase ( has ) gene ( hasa ) was cloned into the cloning vector puc19 by pcr using the total dna sample of s. equi as template
本文研究代謝工程理論，對本實驗室的一株ha生產菌（ streptococcusequi ）進行了ha合成與分解關鍵酶基因及功能的研究。 -
The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。 -
Cloning of rejection related gene and construction of antisense gene transferring vector
玉米溫敏核雄性不育相關基因克隆 -
Streptomyces low copy number plasmid scp2 * is also employed for the cloning and recovery of large dna fragment from streptomyces. as scp2 * can accommodate large dna insertions and is efficiently transmissible among its permissive hosts such as s. lividansbut seemed to be unable to replicates in streptomyces sp. fr - 008, it might be a suitable vector for the cloning of the entire fr - 008 pks gene cluster through gene replacement followed by conjugation with 5
Scp2 *可以在變鉛青鏈黴菌等許可宿主內復制並在許可宿主間有效轉移，但似乎不能夠在鏈黴菌fr - 008中復制，因此可能適合於在鏈黴菌fr - 008中通過進行基因置換及隨后的鏈黴菌fr - 008和變鉛青鏈黴菌間的接合過程來克隆完整的fr - 008生物合成基因簇。 -
In this experiment, ri gene was amplified by pcr method from recombinant cloning vector pt7 - ri, which had been constructed by dr. yu xiu - ping in this laboratory and sequenced by takara biotechnology ( dalian ) co., ltd.
于秀平博士已經構建了ri的克隆載體pt7 - ri ，測序表明與已知人胎盤ri基因的cdna序列完全相同。本文的工作是在此基礎之上完成的。 -
First, a pair of pcr primers was designed to isolate fmdv - vp1 gene according to the published fmdv - vp1 sequence. after pcr of dna isolated from the tissues, the fmdv - vp1 gene was cloned into cloning vector. positive clones were analysed with restriction enzyme digestions and further identified with sequence analysis
首先，根據已知的fmdv基因序列，設計特異性引物，擴增出fmdv - vp1基因，將克隆到的vp1基因連接到測序載體上後用dna測序儀對該序列進行測序。 -
Recombinant cloning vector of pgem - hc and pgem - ha were constructed by ligating the hc and ha genes into pgem t east vector and transformating into jm109. 3
分別將目的基因hypoderminc和hypodermina與克隆載體（ pgemteasy ）連接並轉化到宿主菌jm109中，構建了重組克隆載體pgem - hc和pgem - ha 。 -
An outline of the procedure for introducing a piece of foreign dna into a cloning vector is shown in figure 1. 3.
圖13列出了引出一段外源DNA進入克隆載體的大概步驟。 -
An outline of the procedure for introducing a piece of foreign dna into a cloning vector is shown in figure 1. 3
圖1 3列出了引出一段外源dna進入克隆載體的大概步驟。 -
In genetic engneering, nonviral dna can be inserted into a phage, which is then used as a cloning vector
在基因工程中，沒有病毒的dna可以被插入到噬菌體中，用作克隆載體。
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