column chromatography 中文意思是什麼

column chromatography 解釋
柱層析法
  • column : n 1 【建築】圓柱；圓柱狀物〈如煙柱〉。2 【軍事】縱隊 （opp line）; 隊；（艦隊的）縱陣，縱列，艦列...
  • chromatography : n. 【化學】層析，色層（分離）法。
  1. Fourthly, according to the activity, collect, dialyse, freeze, dry respectively the sod protein through deae - sepharose column chromatog - raphy ; process the sod protein through sephacryl s - 200 column chromatography with the preceding method. at last, process the pure sod protein with same functional enzyme electrophoresis and active dye, isoelectric focusing electrophoresis and sds - page

    將粗酶液過deae -瓊脂糖柱層析，得三個活力峰，分別收集、透析、乾燥濃縮后；再上sephacryls - 200凝膠柱層析，按與deae -瓊脂糖柱層析后同樣的方法收集處理。
  2. According to the polarity, the residue was isolated with petroleum ether, chloroform, ethyl acetate, and n - buoh, respectively. the n - buoh fraction, confirmed as neuroactive component, was subjected to sephadex lh - 20 column chromatography to provide an extract fraction, as a buff powder, which could induce neurite outgrowth in rat pheochromocytoma pc12 cells in a dose - dependent manner up to 50 mg / l

    將菟絲子乾粉用75乙醇浸泡后，減壓蒸干后得到褐色漿狀物，經石油醚、氯仿、乙酸乙酯、正丁醇萃取，經柱層析后，再用葡聚糖凝膠對有效成份進一步純化，獲得了菟絲子中能誘導pc12細胞分化的活性組分。
  3. We got 4 parts from the extraction of astragalus variabilis bunge through systemic research ways of phytochemistry, which respectively are the part of petroleum ether, the part of petroleum ether, the part of etoa and the part of n - buoh the part of n - buoh are isolated by the successively methods of silica - gel column chromatography, tlc and sublimation

    摘要採用植物化學成分系統研究的方法，從變異黃芪醇提總浸膏中分段萃取得到石油醚提取部分、氯仿提取部分、乙酸乙酯提取部分和正丁醇提取部分。
  4. Bubble column chromatography

    氣泡柱色譜法
  5. The powder was then applied to a silica gel column chromatography charged with 80 % acetone in water and eluted with the same solvent. after concentrating and drying, relatively pure fr - 008 was obtained. hplc assay a comparison between antibiotic fr - 008 and candicidin has been made by studying the hplc separation profiles of antibiotic fr - 008, candicidin and a mixture of both

    對鏈黴菌fr - 008和灰色鏈黴菌imru3570的rflp分析發現，它們的染色體具有很高同源性； pfge的比較研究發現，這兩個菌株的明顯區別是鏈黴菌fr - 008攜帶有兩個線性質粒，而在灰色鏈黴菌imru3570中則沒有這兩個線性質粒。
  6. Calmodulin - dependent cyclic nucleotide phophodiesterase was prepared from bovine brain by two - step deae - cellulose column chromatography

    摘要通過兩次離子交換柱層析，從牛腦中制備鈣調素依賴性的環核苷酸磷酸二酯酶。
  7. - acetolactate decarboxylase is purifed from cell extract by 50 % - 80 % ammonium sulfate - fractionation, 50, 2min heat treatment and deae - sepharose fast flow column chromatography, which we study the different ph and different buffer of deae - sepharose fast flow column chromatography and conclude ph 6

    對其酶學性質進行了研究。 -乙酰乳酸脫羧酶經50 80硫酸銨分級沉澱、 50 ， 2min熱處理、 deae - sepharosefastflow離子交換柱層析方法分離純化。
  8. - acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms. the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product. in this paper, - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation, heat treatment, deae - sepharose fast flow column chromatography

    本文對來源於枯草芽孢桿菌（ bacillussubtilis ） 3226 - 5的-乙酰乳酸脫羧酶經硫酸銨分級沉澱、熱處理、 deae - sepharosefastflow離子交換柱層析等分離純化步驟，得到sds - paeg電泳純，通過n末端氨基酸序列分析驗證酶蛋白的純度。
  9. Results galangin was obtained by on - step column chromatography of polyamide only once

    結果運用聚酰胺柱層析次分離，獲得有效成分高良姜素。
  10. This paper concentrates steranes and terpanes in high mature - over mature oil and source rock using distillation method and identifies with column chromatography and chromatography - mass spectrum, achieving better identification effect

    用精餾法對該類原油和生油巖中的甾萜類化合物進行富集，然後通過柱色層分析和色譜質譜鑒定，得到了較為理想的甾萜類化合物鑒定效果。
  11. The methods such as distn. and silica gel column chromatography were used to sep. the various components of the thickener, and the ir spectrometry, gc - ms combination and esi - ms were used to certify the structures of the different components

    用蒸餾和矽膠柱色譜等方法對增稠劑中的各組分進行分離，並用紅外光譜法、氣質聯用和電噴霧質譜法對各組分進行結構鑒定。
  12. An active metabolite was obtained by purification with precipitated by ethanol, sephadex g - 25 gel, deae - cellulose ion exchange resin and silica gel column chromatography

    經乙醇（ 95 ）沉澱、 sephadexg - 25凝膠層析、 deae -纖維素離子交換層析和硅膠層析純化，得到抑制黑麴黴生長的單一組分。
  13. Further investigation on the chemical constituents of the extractions was carried out using column chromatography and gc - ms techniques, and the structures of 12 compounds were identified

    其中，補血功能與藏藥典中記載的蕨麻「益氣補血」作用是完全一致的。
  14. After the acet is vaporized, the active substance in water is gotten. and which is vaporized at low temperature. then the crude active substance is purified by column chromatography on sephadex g - 75. after a series of purifications again, we could get some white powder at last. though the active substance is diluted to50 g / ml, the activity is still checkeded - up through phyto phtnora casicileon. the purified active substance is insensitive to heat, resistant to chloroform 、 ethanol and the orhers. in addition, the active substance is sensitive to high ph ( 10 ~ 14 ), but it is not sensitive to low ph ( 1 ~ 5 ). furthermre, when the ph is made to low again, the activity of it ' s comes back

    用蒸餾水對菌體稀釋；加入適量吸附樹脂在150rpm 、 28下振蕩吸附4h ， 80 %的丙酮解吸，過濾解吸液得到活性物質的澄清溶液，旋轉蒸發儀旋轉蒸發去處丙酮，經sephadexg - 75分子篩層析得單一活性峰，收集峰值部分樣品液經冷凍乾燥得到淡褐色粉末，該活性物質用丙酮充分洗滌、甲醇-乙醚重結晶獲得略帶微黃的白色粉末，該活性物質50 g / ml仍可對蘇雲金芽孢桿菌hd - 1產生明顯的抑制作用。
  15. The quality control and evaluation for the separating and purifying process of steroidal saponin in rhizoma pardis using column chromatography of macroporous resin

    大孔吸附樹脂柱色譜法分離純化重樓皂苷過程中的質量控制與評價
  16. Methods the oil in the seed of panax ginseng c. a. mey was extracted by silica column chromatography, and were analyzed by gc - ms

    方法採用硅膠柱層析法分離出林下參種子油，應用gc - ms技術進行成分分析。
  17. Herba ephedrae was saturated with 0. 5 % hydrochloric acid, the extract was extracted with ether and chloroform respectively, from which ephedrine was separated and purified by column chromatography

    以0 . 5 %鹽酸浸泡麻黃草，分別用乙醚、氯仿萃取麻黃粗提物，通過柱色譜法分離提純麻黃堿。
  18. 3. successful purification of the crude ha was achieved by anion exchange resin deae - cellulose column chromatography using 0. 6mol / lnacl solution as an eluant

    3 .採用陰離子樹脂deae一纖維素對透明質酸粗品進行純化，當naci為0 . 6mol / l時，蛋白質含量較低。
  19. 3. the extracellular phb depolymerase was purified from 09 by using hydrophobic column chromatography and gel filtration technique in sephadex g - 100. the specific activity of the purified enzyme was increased by 37. 9 folds over crude extract, and the recovery yield was 8. 9 %

    以粗酶液為起始，經硫酸銨分級沉澱、 sephadexg - 100凝膠過濾后，分離純化了該酶，純化倍數約為37 . 9 ，酶活力回收率8 . 9 。
  20. The crude cellulases from liquid fermentation of b - 6 and ass. 3711 were isolated and purified by ( nh4 ) so4 precipitation, sephadex g - 100 and deae - sepharose cl 6b column chromatography. the cmcase components were purified and some of their physical and chemical properties were studied

    本文將液體發酵的酶液經硫酸銨分級沉澱、柱層析后得電泳純cmcase組分，並對as3 . 3711和b - 6來源的cmcase酶解動力學和理化性質作了比較研究。
分享友人
分享到 Line

分享到臉書