comet cells 中文意思是什麼
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Dna damages caused by so2 and lead acetate were studied with the single cell microgel electrophoresis technique ( or comet assay ) in order to confirm the damaging degree of lead ( as an important component of atmosphere particle matter ) on dna from male mice exposed to so2. the migrating distances of dna of brain, lung, spleen and kidney cells of mice increased significantly, compared to the control group under conditions of single and combined poisoning of so2 ( 42mg / m3 ) and lead acetate ( 0. 2 % ), and lead could strengthen dna damage degree by so2 in nuclear dna of brain, kidney, spleen cells. damaging degree of so2 on nuclear dna of lung cell of mice was more severe than that of lead
為了明確大氣顆粒物中的重要組分? ?鉛在二氧化硫所致dna損傷中的作用程度，利用單細胞凝膠電泳技術（ singlecellgelelectrophoresis ， scge ，或稱彗星實驗， cometassay ）研究了鉛與二氧化硫的聯合污染，結果表明在42mg m ~ 3so _ 2和0 . 2醋酸摘要一abstract鉛單獨及聯合染毒條件下，小鼠腦、肺、腎、脾細胞dna遷移距離均比對照顯著增加;鉛加劇了50 :對腦、腎、脾細胞核dna的損傷程度; 50 :對肺細胞核dna的損傷程度要比鉛的損傷大，小鼠肺細胞核dna遷移距離在50 :和醋酸鉛聯合作用組與醋酸鉛單獨作用組間有極顯著性差異（ p < 0 . 01 ） ，而與502單獨作用組間沒有顯著性差異。 -
Research on the comet assay in vitro by plating hepg2 cells in 96 - well plates
2細胞體外彗星試驗方法的研究 -
Effect of formaldehyde on dna crosslinking of v79 chinese hamster cells detected by comet assay
氟對鋁作業工人的健康影響 -
Standard guide for determining dna single - strand damage in eukaryotic cells using the comet assay
用彗星分析法測定真核細胞中dna單線損傷的標準指南 -
Then were electrophored. the extent of dna migration were measured. index - percentage of " comet " cell and " comet tail " were analysed that indicated when vero cells were treated with 2 u g / ml quinocetone vero cells got midium - grade damage, and were treated with 6 u g / ml olaquindox vero cells got midium - grade damage
喹乙醇染毒劑量在2 10 g ml時細胞存活率90 ，以2 10 g ml劑量的喹乙醇染毒處于對數生長期的vero細胞，后經過電泳，通過分析彗星樣細胞發生率和彗尾長短等指標，結果表明染毒劑量在6 g ml對dna造成中度損傷。 -
Index - percentage of " comet " cell and " comet tail " were analysed that indicated the optimum time is 2 ~ 3h. the degree of dna damage by quinocetone and olaquindox were detected by scge assay, when vero cells were treated with 1 ~ 5 ug / ml quinocetone the percentage of live cells was above 85 % and with 2 - 10ug / ml olaquindox the percentage of live cells was above 90 %
喹烯酮染毒劑量在1 5 g ml時細胞存活率85 ，以1 5 g ml劑量的喹烯酮染毒處于對數生長期的vero細胞，后經過電泳，通過分析彗星樣細胞發生率和彗尾長短等指標，結果表明染毒劑量在2 g ml對dna造成中度損傷。 -
To solve the problem of wrong or inaccurate analyses of severely damaged comet cells by the comet assay image analysis macro software, to implement the function of manual correction to the macro, and to improve the robustness and user friendliness of the software
本研究還增加了對分析錯誤的數據進行人為校正的功能，由操作者重新指定正確的彗星頭中心，從而把錯誤數據糾正為正確數據，併入統計分析。 -
Moreover, the improved one has manual correction function, as well as better robustness and user friendliness. for comet cells with 0 ~ 2 dna damage grades, the results of the original and improved software were statistically different, however, with no biological significance
實驗結果改進后的軟體對于原軟體分析錯誤的圖像在大多數情況下都可以正確分析，同時擁有了錯誤數據的人工校正功能，程序魯棒性有所增強，更加方便易用。
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