concatemer 中文意思是什麼
concatemer
解釋
多聯體,串聯體,連環體-
It was established that grb - ast3 concatemer are more easily expressed in the way of fusion expession
初步認為grb - ast _ 3串聯體基因適宜採用融合表達載體表達。 -
Is encoded by grb ~ ast7. the expression analysis of the concatemer was proceeded in the e. coli pet expression system
本文主要工作就是構建grb - ast _ 7串聯體，將構建后的串聯體導入大腸桿菌進行表達分析。 -
Neuropeptide ast2 is encoded by grb - ast3. pcr methods were tried to construct grb - ast3 concatemer. the expression analysis of the concatemer was proceeded in the e. coli pet expression system
本論文採用改進的pcr 「自身模板-引物」法進行構建方法構建grb - ast _ 3串聯體，並將拼接好的串聯體轉入大腸桿菌進行表達分析。 -
Concatemer were constructed by polymerization with t4 dna polymerase and primer - templated pcr with high fidelity dna polymerase. one concatemer of 360bp, 9 copies of grb - ast3, were obtained from the first pair of primer and three ones of 515bp, 750bp, 792bp, from the second one
從第一對引物的拼接產物中克隆到一個360bp含有9個拷貝數的grb - ast _ 3的串聯體，從第二對引物的拼接產物中克隆到三個分別為515 、 750 、 792bp的grb - ast _ 3的串聯體。 -
In this method, high fidelity dna polymerase ( pyrobesf * dna polymerase ) was used to ensure efficient and accurate amplification. after cloning and sequencing of the pcr products, one concatemer contains seventeen copies of grb ~ ast7 was attained. the grb - ast ? concatemer was cloned into pet22b expression vector
先用t4dnapolymerase構建成模板，再通過高保真dna聚合酶（ pyrobesttmdnapolymerase ） pcr進行擴增，從中得到一個含有17個拷貝的grb - ast _ 7的串聯體。
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