concentration expression 中文意思是什麼

concentration expression 解釋
濃度表示法
  • concentration : n. 1. 集中。2. 【化學】提濃，蒸濃，濃縮；濃度；稠密度；【礦物】汰選，選礦，富化。3. 集中注意，專心。
  • expression : n 1 表現，表示，表達。2 詞句；語句，措辭，說法。3 表情，臉色，態度；腔調，聲調。4 【數學】式，符...
  1. First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected

    目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。
  2. To investigate the consequence of this interaction, aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector. confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region. moreover, this interaction inhibited the concentration of tle1 into nucleus

    在構建了紅色熒光蛋白aes表達載體后，將其與tle綠色熒尤蛋白載體共轉染細胞，共聚焦顯微鏡觀察發現這兩種分子在胞漿中有共存現象，而且aes的表達可抑制tlei向胞核內的聚積。
  3. When ultima concentration of iptg was 1. 0mmol / l and induced for 3 hours, the protein expression quantity was the most candidate : zhao chunli major : basic veterinary science supervisor : prof. haoyanhong prof. liqingzhang

    對表達蛋白的定量分析得出，當iptg終濃度為1 . 0mmol l ，誘導3個小時蛋白表達量最大。
  4. No significant difference was observed on the expression levels of cein at different iptg concentration and differet times in recombinant e. coli. jm109 and recombinant e. coli

    Blzi進行誘導表達發現，不同的iptg濃度誘導對外源基因cellz的表達量影響不大，不同的誘導時間對外源基因cell 。
  5. The expression efficiency difference between ped5 and pcdhfrl, a vector utilizing cmv enhancer / promoter ( pcmv - ie ) for foreign protein production, was analyzed using human interferon - p ( ifn - ) gene and human secreted alkaline phosphatase ( seap ) gene as reporters. when analyzed in transient expression, ped5 showed a little more protein produciton than pcdhfrl. however, in continuous expression, when serum concentration was lessened to slow down cell proliferation, ped5 expressed 3. 1 times more reporter proteins than pcdhfrl, which implied that pef - io was less affected by cell cycle status in contrast to pcmv - ie, making ped5 a good expression vector for foreign protein production

    應用人-干擾素（ ifn - ）和人分泌型堿性磷酸酶（ seap ）基因作為報告基因，對含有巨細胞病毒即早期啟動子（ p _ （ cmv - ie ） ）的表達載體pcdhfr1和ped5表達外源蛋白的能力進行了比較，發現對于瞬時表達， ped5略好於pcdhfr1 ；在穩定表達中，通過降低血清濃度，使細胞增殖緩慢，這時ped5表達外源蛋白的能力較pcdhfr1高3 . 1倍。
  6. The recombinant vectors were transformed into e. coli m15 respectively and the expression was induced based on the optimal values of the iptg concentration incubation temperature and induction time determined in the previous section

    根據優化確定的iptg誘導濃度、誘導溫度和時間進行誘導表達。 5的積層膠， 15的分離膠，變性聚丙烯酰胺凝膠電泳（ sds - page ）檢查表達情況。
  7. Concentration - dependent effects of atorvastatin calcium on the expression of e - selectin and intercellular adhesion molecule - 1 in cultured human umbilical vein endothelial cells

    選擇素和細胞間黏附分子1表達的濃度依賴性
  8. Here we managed to make cultured mice peritoneal macrophages be directly influenced by oligochitosan, and be stimulated by ifn - r before oligochitosan added, then measured the changes of gene transcription and translation level of both il - 1 and imf - a, respectively by methods of relatively quantitive rt - pcr and elisa. first, rt - pcr results showed that 18 hours was the most effective time and 40ug / ml was the most effective concentration of oligochitosan, then by the same method, confirm that 4hours is the most effective time and loou / ml is the most effective concentration of ifn - r stimulating. because ifn - r can enhance il - 1 and tnf - a gene expression of macrophages alone, so add ifn - r to microphages alone for 22 hours, then examined by rt - pcr, the results showed that il - 1 and tnf - a gene expression have no remarkable difference compared with the blank contrast group

    此外，由於ifn y單獨作用也可促進兩種細胞因子基因表達，故在巨噬細胞中加入ifn y單獨作用22h ，再經阿一pcr檢測，發現加ifn y的實驗組細胞的幾一lp和tnf a基因轉錄水平與空白對照組相比較無顯著性差異，可見，殼寡糖和ifn v對巨噬細胞il lp和tnf一口基因轉錄水平的影響在作用時間上無一致性，在殼寡糖作用最適時間時，僅受ifn y刺激的巨噬細胞il lp和tnf q基因轉錄己下降至刺激前水平，因此可以認為， ifn y的加入僅起到對巨噬細胞預刺激使之處于敏感狀態的作用，有利於增強殼寡糖對巨噬細胞的作用。
  9. Flexibility in dispersal, concentration and shifts of position is a concrete expression of the initiative in guerrilla warfare, whereas rigidness and inertia inevitably lead to passivity and cause unnecessary losses

    分散、集中和轉移的靈活性，都是游擊戰爭具體地表現主動性的東西；死板、呆滯，必至陷入被動地位，遭受不必要的損失。
  10. The main factors influencing agrobacterium - mediated genetic transformation of portulaca grandiflora hook, are as follows. the concentration of kanamycin of devision leaves and the calli were 100mg / l and 200mg / l, respectively. calli infection can gain more gus gene transient expression and resistant tissuses than devision leaves

    對松葉牡丹進行遺傳轉化研究發現，採用葉片作為外植體時， kan的濃度100mg / l就可抑制愈傷的誘導，而用愈傷組織作為材料感染時濃度要達到200mg / l才可。
  11. The expression of pain and concentration returned to piggy's face.

    痛苦和專注的神色又回到了豬崽子的臉上。
  12. According to the effective field expression which has been derived in former, the phenomenon that the tangential component of magnetic leakage field has maximum value and the normal component of magnetic leakage field acquires zero value at the stress concentration zone of positive magnetostriction ferromagnetism materials under the application of tensile stress and negative magnetostriction ferromagnetism materials under the application of compressive stress is explained theoretically through analyzing and discussing

    根據所得到的地磁場中受力鐵磁性材料有效場表達式，通過分析討論，解釋了受拉力作用的正磁致伸縮鐵磁材料和受壓力作用的負磁致伸縮材料在應力集中處漏磁場切向分量出現最大值、同時法向分量為零值的現象。
  13. By investigating the specialty of functions in the expression, the following conclusion was drawn : particle concentration was the dominant factor that enforced ablation, and angle contributed to ablation via cutting the char layer

    通過分析關聯式中各項函數特性，得到粒子聚集濃度是影響燒蝕量的主要因素，而角度對于燒蝕量的貢獻主要體現在對絕熱層炭層的剪切破壞上的結論。
  14. The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively

    豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。
  15. Bth had a higher efficiency to induce gus expression than sa indicated by gus staining strength and had no phytotoxicity to plant within effective concentration. 5

    化學誘導劑bth的誘導效率高於sa ，且在有效誘導濃度范圍內對植株沒有生理毒性或所產生的生理毒性難以從表象觀察到。
  16. The strains with high expression level is selected according to the result of expression experiment with the methods introduced by expression guideline, and its expression condition is op timized in three aspects, namely time of inducing, ph value and inducing concentration of methanol

    按表達手冊進行表達實驗，挑選出具有高分泌表達水平的菌株，並採用不同的誘導時間、 ph值、甲醇誘導濃度進行表達比較，優化出適宜的表達條件。
  17. The recombinant was identified by dual enzyme digestion and the direction of cd40 / pcdna3 was analysed with t7 primer. after being packed by lipofectaminetm2000, the recombinant was transfected into b lymphocytes. cd40 expression on membrane, cell proliferation and antibodies concentration were detected with flowcytometry, mtt colorimety assay and el1sa, respectively

    以脂質體為介質瞬時轉染健康人及sle患者b細胞系，利用流式細胞技術檢測膜cd40的表達情況，並利用mtt比色法檢測細胞的增殖能力， elisa法檢測培養液的ig濃度，以研究b細胞在cd40被抑制以後增殖能力、抗體分泌的改變。
  18. Expression was induced by adding iptg to a final concentration of imm and incubation with shaking at 30 for 3 h

    以2 %比例接種入新鮮mg培養基，培養至對數中期，加入iptg至終濃度為1mm ，在溫度為30的條件下誘導表達3小時。
  19. It was estimated that the yield of the e2 fusion protein in culture supernatant of recombinant p. pastoris could be reached 0. 34g / l. the optimal expression conditions were explored for the ph, aeration rate, final concentration of methanol and kinds of growth media. we conclude that the higher quantity of e2 protein can be gained at ph4. 0 - 6. 0, 1 % methanol as the final concentration, mm growth media and high aeration rate

    通過對表達條件如ph值、通氣量、誘導甲醇終濃度、培養基的選擇等條件的探索，可確定豬瘟病毒hl - ly株e2基因表達的理想條件為：最適ph值在4 . 0 6 . 0之間，最適培養基為mm培養基，最佳誘導的甲醇終濃度為1 ，加大通氣量時表達量較好。
  20. Induced either by iptg or l - tryptophan in the absence of easily metabolized carbon such as glucose the strain bl21 ( de3 ) / pet28c - tnaa can express tryptopanase. the fermentation conditions were optimized respectively. in the presence of glucose and iptg as induce agent, the concentration is the crucial factor for expression of active tryptophanase. if the iptg concentration is less than 0. 2mm, the optimum temperature is 37 lower temperature is necessary to obtain active tryptophanase in the case of higher concentration of iptg

    用iptg誘導表達實驗結果表明：利用t7啟動子在胞內能表達出有活性的酶； iptg與溫度的合適組合可以得到較高活性的色氨酸酶，在0 . 2mm以下濃度的iptg誘導時， 37是最適溫度，而用較高濃度的iptg誘導，低溫是表達有活性的色氨酸酶的必要條件，而且誘導時間要短。
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