copy dna 中文意思是什麼
copy dna
解釋
復制脫氧核糖核酸-
The characteristics of tm - 22 expression presented in transgenic tobacco : 1 ). virus specificity in either homozygote or heterozygote ; 2 ) tm - 22 gene integrated in tobacco genomic dna with single copy and in inheritance and segregation to progenies on the mendel role ; 3 ). transgenic line with tm - 22 promoter ( ptm47 ) showed infected symptoms with cell death distinguished to one with 35s promoter ( ptm49 ) after inoculation with tomv - 2a
其次，通過氨基酸序列和結構的比較，確定tm - 2 ~ 2基因的編碼蛋白與tomv病毒在抗病反應中相互識別的特異氨基酸及其功能；然後，應用重組dna技術，互換tm - 2 ~ 2基因和tm - 2基因的對應結構域，構建嵌合基因，獲得嵌合蛋白表達的轉化體，驗證tm - 2 ~ 2編碼蛋白中變異氨基酸的作用。 -
1. because the taxonomic division is rather complex and has been much disputed and revised, in this part, we will review the classification and phylogeny of families, subfamilies and tribes of anseriformes based on morphology, ethology, osteology, mitochondrial and nuclear dna restriction fragment length polymorphism, single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition, classification and phylogenetic relationships of the families, subfamilies and tribes of anseriformes. the controversial questions and deficiency in the systematic studies of anseriformes were pointed out
具體包括以下幾個部分： 1 、針對雁形目鳥類異常復雜的分類狀況及分類上存在的爭議，根據雁形目鳥類的形態學、行為學、骨骼學、角蛋白、線粒體與核dna酶切片段長度多態、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究，對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統發生關系進行綜述，分析系統學研究中存在的不足，提出了雁形目鳥類分類中急需解決的問題。 -
Cc was cloned from a cell taken from near the ovary of a tortoiseshell cat, but she is not a carbon copy of her dna donor
茜茜是由一隻花貓卵巢旁的細胞克隆而來，但是她和她的"母體"並非長得一模一樣，她們的皮毛花紋還是有所不同的。 -
Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。 -
Streptomyces low copy number plasmid scp2 * is also employed for the cloning and recovery of large dna fragment from streptomyces. as scp2 * can accommodate large dna insertions and is efficiently transmissible among its permissive hosts such as s. lividansbut seemed to be unable to replicates in streptomyces sp. fr - 008, it might be a suitable vector for the cloning of the entire fr - 008 pks gene cluster through gene replacement followed by conjugation with 5
Scp2 *可以在變鉛青鏈黴菌等許可宿主內復制並在許可宿主間有效轉移，但似乎不能夠在鏈黴菌fr - 008中復制，因此可能適合於在鏈黴菌fr - 008中通過進行基因置換及隨后的鏈黴菌fr - 008和變鉛青鏈黴菌間的接合過程來克隆完整的fr - 008生物合成基因簇。 -
I have used low copy pbin19 and single copy pmw755i5j binary vectors as backbone plasmids, to create a gene targeting insertion vector designated gfp tnos. after agro - infiltration into transgenic nicotiana benthamiana 16c, progeny were analyzed genetically for phenotypic changes, sirna accumulation, and dna methylation
採用農桿菌浸潤法（ agro - infiltration ）感染轉基因本生煙16c ，並對同源基因瞬時表達所引起的植物表型變化、小分子rna的產生、 dna甲基化程度、以及相關性狀在後代中的遺傳情況進行了檢查。 -
Kurstaki strain hd73, were inserted into two copy sets of res sites. the res sites have same direction. when - the recombinant plasmid was introduced into crystal negative b. thuringiensis host bmb171, antibiotic resistance genes and other non - 5, thuringiensis dna can be selectively eliminated after the selection by antibiotic resistance marker
將crylac10基因或壯觀黴素基因和蘇雲金芽胞桿菌的質粒復制起始區oril030連接在一起，置於兩個同向的解離區之間，再將基因操作中所必需的大腸桿菌質粒復制起始區和抗生素標記基因等與之相連構成解離載體。 -
Was obvious to all to be detailed the establishment gram venereal diseases kj medicinal preparation is one high active living thing medium which in the outside too bareness oxygen, the low attraction, under the high radiation special environment in the middle of the copy process has the completely block the viral dna duplication, skin base first floor and the dermis cellular layer comprehensively sweeps clean the virus
Annlic安立克kj劑是在外太空無氧氣低引力高輻射的特殊環境下生成的一種高活性生物介質，具有超越傳統產品20倍的超強大抗病毒作用，它在病毒dna復制過程當中具有超強干擾作用，可完全阻斷病毒dna復制，並可以透皮吸收深入皮下，直達皮膚基底層和真皮細胞層全面掃清病毒。 -
Annlic the establishment gram kj medicinal preparation is one high active living thing medium which in the outside too bareness oxygen, the low attraction, under the high radiation special environment produces, has the surmounting legacy product 120 time of ultra formidable anti - virus functions, it has the ultra strong jamming function in the middle of the viral dna copy process, may completely block the viral dna duplication, and may pass the skin absorption, be thorough hypodermic, goes directly to the skin base first floor and the dermis cellular layer comprehensively sweeps clean the virus
Annlic安立克kj劑是在外太空無氧氣低引力高輻射的特殊環境下生成的一種高活性生物介質，具有超越傳統產品20倍的超強大抗病毒作用，它在病毒dna復制過程當中具有超強干擾作用，可完全阻斷病毒dna復制，並可以透皮吸收深入皮下，直達皮膚基底層和真皮細胞層全面掃清病毒。 -
Results : designed and synthesized the coding gene of echistatin. and constructe its single copy expression vector, identify the vector by dna sequencing. but it could not express echistatin in e. coli. designed and synthesized the modified coding dna of echistatin, and constructe its two copy expression vector, identify the vector by dna sequencing, but it could not express echistatin in e. coli
結果設計併合成了echistatin編碼基因dna序列，構建了echistatin單拷貝表達載體，經dna測序鑒定正確后， sds - page及western - blotting結果表明： echistatin在上述菌株中未獲得高效表達。 -
Proper multi - copy gene was selected and cloned into puc19 vector. restriction enzyme analysis and dna sequencing confirmed that 5 - copy gene was correctly inserted into the vector
選取合適拷貝數的串連重復基因，將其克隆至puc19載體，雙酶切、 pcr擴增和dna測序證明串連重復基因構建成功且基因方向相同。 -
The unequal exchange of dna in a reproductive cell and subsequent mutation of an extra copy of a gene for a pigment sensitive to long wavelengths resulted in the creation of a second long - wavelength - sensitive pigment, which had a shift in its wavelength of maximum sensitivity
生殖細胞的dna不對等互換，以及長波長色素重復基因的后續突變，創造出了第二種對長波長敏感的色素，只不過最敏感的波長已經變了。 -
Totally 99 transgenic rice plants from 125 resistant calli of 191 calli were obtained and pcr assay showed that 80. 3 % of them were positive. the result of southern blotting analysis for primary plants revealed that each transgenic plant contained a average of 2. 5 copy of t - dna
對抗性植株進行pcr擴增檢測，結果表明有80 . 3的抗性植株為陽性植株； southern雜交結果進一步證明了質粒的t - dna已經整合到水稻的基因組中，拷貝數為1 4個，平均每棵轉化植株有2 . 5個拷貝。 -
Methods : 1. expression and purification of recombinant human ctla4 extracellular domain : a 400bp dna fragment of ctla4 extracellular domain was obtained from the pctla4 / ig plasmid with genetical technique. then, this dna fragment was inserted into ppic9 plasmid to construct the single copy plasmid of yeast expression system ( ppic9 - ctla4125 ). furthermore, the multi - copy plasmid ( ppic9k - ctla4125 ) was constructed
重組人ctla4胞外區蛋白在酵母gs115中的表達和純化：首先通過基因操作技術，從pctla4八g質粒中擴增400hp的ctla4胞外區片段；將ctla4胞外區片段插人ppicg質粒中獲取單拷貝酵母表達質粒ppicg ctla4125並進一步構建多拷貝酵母表達質粒ppicgk （ 。 -
In addition, molecular experiments indicated that resistance was not relevant to copy number or to insertion sites, but instead to the repeated gfp structure and to methylation of genomic dna. pvx vectors were constructed that contained the s6 gene of rbsdv vi and the 14 kd fragment of bnyvv rna2
分子檢測結果顯示， gfp3植株中普遍存在gfp基因的沉默現象，且gfpdna的甲基化程度明顯高於gfp1植株，說明這些植株對于病毒的抗性與目的基因的插入位點及拷貝數和無關，但與串聯重復的gfp基因結構形式密切相關。 -
They also scanned the dna to search for copy number variations, which are submicroscopic insertions and deletions of genetic material that scientists believe may be linked to autism and other diseases
他們也掃描dna來尋求復制數的變化趨勢，發現科學家提出的遺傳學材料的亞微觀的插入或是刪除，可能與孤獨或其他疾病有關系。 -
A mutant probably harboring more plasmids can grow better on nitrobenzene selective medium than other strains. the plasmid harbored in this mutant is also a few kb smaller than others. so a probability is supposed that a certain plasmid dna fragment deletion made the number of plasmid copy change, which affected the mutant growth on the selective medium
一株質粒含量較高的突變株在硝基苯選擇培養基中的生長情況要好於其它菌株，其所含的質粒也略小一些，因此我們推測，某個質粒片段的缺失造成了質粒拷貝數的變化，從而影響了它的生長特性。
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