cryopreservation 中文意思是什麼
cryopreservation
解釋
低溫保存-
4 ) cryopreservation had a little influence on hpfl ' function, and the influence aggravated as cryopreservation time went on. when hpfl were recovered, their activity and attachment efficiency declined, and the excretion peak value of alb descended
4 ）凍存復甦對hpfl的生物學功能有一定影響，隨著凍存時間的延長，復甦后的細胞活性和貼壁率有一定下降，白蛋白分泌峰值亦有所降低。 -
Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus, two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196. after 6 days cryopreservation, one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other, cryopreserved for 13 days, was 52
以細胞培養液m199 （含20 %的小牛血清）為稀釋液， dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣，採取先慢后快的方式，冷凍保存兩組草魚囊胚晚期細胞於一1 %的液氮中。第一組冷凍保存6天後解凍，成活率為72 % ，第二組冷凍保存13天後解凍，成活率為520 / 0 。 -
Cryopreservation of flexor tendon by vitrification in chicken
雞肌腱的玻璃化冷凍保存 -
Different from mammals, the early embryos of fish can not be preserved for the long period at the very low temperature ( - 196 ). therefore, three methods were usually applied to cryogenic preservation of the fine and rare species of fish : 1 ) perserving fish spermatozoon in cryogenic condition. researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects ; 2 ) combining with the techniques of cell engineering ( nuclear transplantation and electric fusion etc. ), and through the process of culturing histiocyte of fish, cryopreservation and re - culture after thawing, carrying out somatic cell breeding of fish. the past studies showed that the nucleolus of somatic cells of fish have totipotency
多年來，國內外學者對各種魚類精液的冷凍保存進行了大量的系統研究，目前這項技術已達到實用水平，並日益發揮作用；二是對魚類培養的組織細胞冷凍保存，通過魚類細胞的培養、超低溫凍存、解凍后再培養過程，結合細胞工程技術（如核移植、電融合等）進行體細胞育種；大量的研究結果表明魚類體細胞核具有發育的全能性，隨著細胞培養技術、細胞工程技術日益發展成熟，完全具備實現魚類物種種質長期保存的理論基礎和技術條件。 -
Effect of opitmal - concentration matrine combined with cryoprotectant on the cryopreservation of immortalized l - 02 hepatocytes
02人肝細胞系凍存保護的效應 -
For the cryogenic preservation of fish, in this paper we made the primary culture of the kidney of allotetroploid crucian carp and primary studies were carried out on cryopreservation culture offish embryo cells derived from misgurnus auguillicaudatus or grass carp, the results of the experiments are as follows : the primary cell culture of the kidney tissue derived from allotetroploid crucian carp was carried out using tissue adherent culture, the primary observations of the growth conditions and morphology of the primary culture and subculture cells which originally come from the kidney tissue were also made
本文主要從魚類種質保存的目的出發，一方面以四倍體鯽鯉魚為材料，對四倍體鯽鯉魚腎臟組織進行初步培養，為建立相應細胞庫及下一步培養凍存的魚類胚胎細胞奠定基礎；另一方面，以魚類組織細胞培養技術為基礎，泥鰍胚胎細胞為材料，對魚類胚胎細胞凍存培養方法進行初步研究，並應用該技術方法對草魚早期胚胎細胞進行凍存培養實驗。報告如下：本文用組織貼壁法對四倍體鯽鯉魚腎組織進行原代、傳代培養。 -
Research progress of cryopreservation of human oocytes
人卵子冷凍保存的研究進展 -
Preliminary study on rabbit semen cryopreservation
兔精液冷凍保存的初步研究 -
Vitrification is a simple and efficient technique for cryopreservation
它是一種簡便、有效、成本低廉的超低溫保存方法。 -
Cryopreservation of calli by vitrification and plant regeneration of rhodiola sachalinensis
高山紅景天愈傷組織的玻璃化法保存及植株再生 -
Cryopreservation of in vitro papaya shoot - tips by vitrification technique and its regeneration
番木瓜莖尖的玻璃化法超低溫保存及其植株再生 -
Objective : to study the value of mixed animo acids and rapid freezing in vessel cryopreservation, and simplify the proceeding schedule of vessel cryopreservation technique, reduce costs of preservation, promote popularization and development of that technique in clinical use
目的：本實驗主要探討復方氨基酸和速凍降溫方式在血管深低溫保存中的應用價值，以期簡化血管深低溫保存技術的操作步驟，降低其保存成本，推動該技術在實際應用中的普及和發展。 -
But in experiment ii, the obtained result of measurement and examination is not better than control group 。 conclusion : mixed animo acids can be safely applyed to vessel cryopreservation instead of calf serum 。 the effect of the groups of the rapid freezing method is not better than the groups of the rate - controled slow freezing
而實驗ii的血管其內皮結構不清晰，內皮細胞超微結構變化較大。結論：復方氨基酸可代替胎牛血清用於血管的深低溫保存.速凍降溫方式對血管深低溫保存效果不及慢凍降溫方式。 -
Cryopreservation by vitrification is a kind of biotechnology sprung up in 1980 ' s. it is superior in simple - equipment and facilitated - operation. vitrification refers to the physical process by which concentrated cryoprotectant and cells treated are totally vitrificated into a ice - free and homogenous state
玻璃化法是指受高濃度復合保護劑處理后的細胞連同保護劑本身都進入一個均一的無冰晶的玻璃化狀態，冰凍保存的細胞受到損傷較小，存活率較高。 -
Most studies focused on the methods of cryopreservation and finding better cryoprotective agent
目前研究焦點在於尋找更好的冷凍保護劑和簡化操作方法等方面。 -
The quality of feeder layer is affected by a lot of factors, such as animal breed, culture medium, passages in vitro and experiment condition, etc. as to the production of feeder layer, there are a few reports about morphological and histologic change when of embryonic body fibroblast when culturing in vitro and cryopreservation, so kunming mouse were chosen as experimental animals and morphological and histologic changes were studied in course of its embryonic body culturing. we expect to offer theoretical foundation to our laboratory for setting up feeder layer storehouse. at the same time, the feasibility of myocardium tissue culturing with fibroblast layer altogether was studied so that established foundation for studied the biological characteristic of heart outside body
小鼠胚體成纖維細胞的培養是制備飼養層的重要途徑，其制備、傳代及冷凍保存均有不同的研究報道，飼養層的質量受許多因素的影響，如動物的品種、培養液、所傳代數及實驗條件等，關于飼養層制備過程中的胚體細胞培養、傳代、冷凍后的細胞形態、組織學等方面的研究報道很少，故本實驗以昆明小白鼠為實驗動物，研究其胚體培養過程中細胞的形態學、組織學等方面的變化，以期為本實驗室建立飼養層細胞庫提供理論依據，同時探討心肌細胞和成纖維細胞層共培養的可行性，以期為心臟生物學特性的體外研究奠定基礎。 -
Study of cryopreservation on castanea mollissima seeds
板栗種子超低溫保存研究 -
The viability test is a key step in cryopreservation
鑒定存活率是超低溫保存技術中的重要環節。 -
Study on cryopreservation of in vitro cultured cabernet france shoot tips
葡萄離體莖尖超低溫保存的研究 -
Effect of cryopreservation on changes in human spermacrosome integrity and ultrastructure
冷凍保存對人類精子頂體完整性及超微結構的影響
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