culture experiment 中文意思是什麼

culture experiment 解釋
培養試驗
  • culture : n. 1. 教養；修養；磨煉。2. 文化，（精神）文明。3. 人工培養，養殖；培養菌，培養組織。4. 耕作；栽培；造林。vt. 使有教養。
  • experiment : n 實驗；試驗；嘗試 （of） a scientific experiment 科學試驗，科學儀器設備。 a new experiment in ed...
  1. Shake - flask culture condition of lysine fermentation were studied with czl. appropriate feeding of organic niteogen sources was favorable for fermentation jn our experiment, the concentations of the yeast extract and beef extract were 15g / l and 25g / l. the initial ( nh02so4 and glucose concentrations suitable for fermentor production ranged form 55g / l and 150 - 180g / l respectively

    通過搖瓶發酵實驗初步確定czl的發酵條件為：初糖： 150 - 180g / l ，採用無機氮源和有機氮源配和使用，最佳組合是：硫酸銨質量濃度為55g / l ；酵母膏質量濃度為15g / l ，牛肉膏質量濃度為25g / l為最佳。
  2. Section two the evaluation of biocompatibility of the acellular dermal matrix by the method of cell culture. the new born rat ' s epdermic cells were cultured with the acellular dermal matrix together as experiment group, while the epdermic cell were cultured simply as control. 24 hours later, under the invert microscope, the epidermic cells anchored well and transparent flat cells were observed in both groups. 7 days later, both cultured cells were taked out and fixed in 95 % ethanol, stained with hematoxylin and were observed under light microscope. many cleaved cells were observed in both groups. during cell culture, no pathogenic microganism was observed. so we considered the acellular dermal matrix was aseptic and had good biocompatibility. section three subdermal implantation of the acellular dermal matrix. 24 rats were used in the experiments. a piece of acellular dermal matrix ( 1. 5 x 1. 5cm2 ) was implanted beneath the dorsum skin flaps of each rat, 1 week, 2 weeks, 3 weeks and 4 weeks after implantation, 6 pieces of acellular dermal matrix were harvested and the size of implanted acellular dermal matrix were measured, the sections were used for he staining and observed under light microscope. the result were as folio wing : 1 - 2 weeks after implantation, the acellular dermal matrix began to adhere to the tissue around and turned red gradually ; 3 - 4weeks after implantation, the acellular dermal matrix adhered closely to the tissue around and could be recognized easily, 1 - 3 weeks after implantation, the size of implanted acellular dermal matrix had no statistical difference ( p > 0. 05 ). 4 weeks after implantation implanted acellular dermal matrix contracted ( p < 0. 05 ). under light microscope, l - 2weeks after implantation, the fibroblast cells infiltrated the acellular dermal matrix and a small amount endothelial cells of vessel and lympho - histiocytic cells infiltrated the acellular dermal matrix. 3 - 4 weeks after implantation, infiltrating blood vessels were evident. so we think that the acellular dermal matrix had low immunological reactions and could induce the infiltration of fibroblast macrophage cell and the endothelial cells of vessel

    結果如下：皮下包埋卜周者，無細胞真皮基質漸與周圍組織粘附，顏色由蒼白轉紅；皮下包埋3周者，無細胞真皮基質與周圍組織緊密枯附，盾晰葉辯；術后卜周，包埋的基質面積變化較包埋前無統計學差異o川0引，術后4周包埋的無細胞真皮基質面積較包埋前縮小j刃刀5 ） 。光鏡下術后卜周，宿主的淋巳組織細胞、成纖維細胞浸入生長，釉附在膠原纖維上，少量血管內皮細胞浸入基質；術后34周，無細胞真皮基質內較多的血管形成，故可認為無細胞真皮基質免疫原性低，能誘導宿主的成纖維細胞、巨噬細胞浸入生長，為一種新型的真皮替代物。第四部分無細胞真皮基質與自體斷層皮片復合移棺的研究， sd大鼠10隻，在其背部卜方造成全厚皮膚缺損的創面
  3. Immature dc generated from mouse bone marrow by culturing in gm - csf and il - 4 for 5 days were incubated with hs - exo or exo for 48 hours, and then were collected for phenotypical analysis and phagocytosis of ova - fitc by facs technique. cytokines in the culture supernatants were quantitated using elisa. in the experiment of mlr to detect

    熱休克誘導細胞表達熱休克蛋白，對exosomes中熱休克蛋白的檢測發現，在exo和hs一exo中均存在hsp60 、 hsp70和hsp90 ，其中hs一exo中hsp6o和hsp90的含量顯著高於exo ， hsp70的
  4. A pool - culture experiment as conducted to study the effects of potassium humate on the growth and active oxygen metabolism of ginger root

    摘要採用池栽試驗方法研究了腐植酸鉀對生薑根系生長發育及活性氧代謝的影響。
  5. This experiment passing to grope for the carbon source constitutes of the culture medium and using t. reesei rut c - 30 induced the expression of # - mannanase ( # - 1, 4 - mannan mannohydrolase ec 3. 2. 1. 78 ). in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t. reesei rut c - 30, then proceeded the variable carbon source ( dragon spruce fiber, com rush pith fiber, wheat straw fiber, wheat straw xylan, corn rush pith xylan, dragon spruce mannan ) to single factor, double factor, three factor, four factor and five factor orthogonal experiment. 1 determined the activity of p - mannanase using locost bean gum as substract by the 3, 5 - dinitosalicylic acid method, and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber, wheat straw xylan, dragon spruce mannan

    在里氏木霉rutc - 30的基礎培養基（ mandels營養液）中加入固定碳源乳糖和槐豆膠，然後將可變碳源（雲杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、雲杉甘露聚糖）進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗，並以槐豆膠為底物用3 ， 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含雲杉纖維、麥桿木聚糖和雲杉甘露聚糖的誘導培養基為最佳培養基，用該培養基培養的里氏木霉（ t . reesei ） rutc - 30使其轉錄的-甘露聚糖酶（ - 1 ， 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ） mrna量能夠滿足rt - pcr的要求。
  6. A synchronous mitotic experiment system that was in an unchanged culture condition was established

    建立了多頭絨泡菌自然同步化細胞分裂的實驗體系。
  7. To study the suitable method for cattle oviduct simple epithelium cells culture, the epithelium cells were isolated by cutting and 0. 25 % trypsinization, the exponential phase of growth cells vigor and growth velocity was determined by mtt method, the viable count was detected by the rejection experiment of trypanblau

    摘要為探討適用於黃牛輸卵管單層上皮細胞的培養方法，採用機械剪取及0 . 25 %胰酶消化的方法分離獲得上皮細胞，取對數生長期細胞進行mtt比色實驗檢測細胞活力和生長速度；臺盼藍排斥試驗檢測活細胞數。
  8. Conclusion a systematic method for preparation of enzyme - mannanse is established, a high productive strain was got after seducing and selecting from nature, confirmed as brachybacterium spa6 research were conducted on medium and culture method of the strain in order to get the suitable cultural condition of fermentation, the experiment result shows the optimium condition is ph7. 0, temperature 36c ; carbon content 2. 5 %, ventilation in abundence, agitation speed 200r / min

    結論1 、以從自然界中篩選出的菌株為出發株，經誘變、篩選，得一高產葡甘聚糖酶菌株，初步鑒定為短桿菌屬brachybacteriumspa6 2 、經誘變、三角瓶培養，該菌株的最適培養條件：培養基ph值7 . 0 ，碳源2 . 5 ，振蕩培養， 200r min ，培養溫度36 ，培養48h 。
  9. In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks

    方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。
  10. The quality of feeder layer is affected by a lot of factors, such as animal breed, culture medium, passages in vitro and experiment condition, etc. as to the production of feeder layer, there are a few reports about morphological and histologic change when of embryonic body fibroblast when culturing in vitro and cryopreservation, so kunming mouse were chosen as experimental animals and morphological and histologic changes were studied in course of its embryonic body culturing. we expect to offer theoretical foundation to our laboratory for setting up feeder layer storehouse. at the same time, the feasibility of myocardium tissue culturing with fibroblast layer altogether was studied so that established foundation for studied the biological characteristic of heart outside body

    小鼠胚體成纖維細胞的培養是制備飼養層的重要途徑，其制備、傳代及冷凍保存均有不同的研究報道，飼養層的質量受許多因素的影響，如動物的品種、培養液、所傳代數及實驗條件等，關于飼養層制備過程中的胚體細胞培養、傳代、冷凍后的細胞形態、組織學等方面的研究報道很少，故本實驗以昆明小白鼠為實驗動物，研究其胚體培養過程中細胞的形態學、組織學等方面的變化，以期為本實驗室建立飼養層細胞庫提供理論依據，同時探討心肌細胞和成纖維細胞層共培養的可行性，以期為心臟生物學特性的體外研究奠定基礎。
  11. This article mainly discusses the large - scaled cultural conditions of clone in gametophyte laminaria. it studies the proper temperature, light time, light intensity, the consumption of nitrogen and phosphorus, the cultural state and inoculating density on the growth of gametophytic clone, the experiment of large - scaled culture had been done under comparatively proper conditions, in the same time, we studied the growth rule of gametophytic clone in the cultural process

    本文主要探討了海帶配子體克隆的大規模培養條件，對海帶配子體克隆生長的適宜溫度、光照時間、光照強度、營養鹽消耗、培養狀態以及接種密度進行了研究，並在相對適宜條件下進行了大規模培養實驗，同時，對海帶配子體克隆在培養過程中的生長規律進行了研究。
  12. 4. after having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time, incubation time and co - culture time, we set up the system of high frequency transformation of tomato cotyledons. then hbmp - 3m gene was transferred into tomato via agrobacterium - mqdiated transformation, and the resistant plants to hyg were obtained. by pcr analysis on part of the putative transformants, we identified that hbmp - 3m gene had been integrated into the genome of part of tomato plants. 5. transferred hbmp - 3 gene into tobacco via agrobacterium - mediated transformation and obtained the resistant plants to hyg. trans genie tobacco plants were confirmed by instantaneous expression of gus gene in calli detection, growth and bio - morphology analysis, hyg - resistant experiment and pcr analysis

    通過pcr檢測證實部分番茄抗性植株中已導入hbmp - 3m基因；人骨形成蛋白一3成熟膚基因和全長基因分別轉化番茄和煙草的研究5 .通過農桿菌介導法將hbmp一3全長基因導入煙草，並且獲得了hyg抗性植株，通過gus基因瞬時表達檢測、轉化植株的生長情況及形態學分析、 hyg抗性鑒定及pc尺檢測，證明目的基因己經整合到煙草基因組中。
  13. Laboratory culture experiment with the same treatments as pot experiment but without plant was carried out synchronously under constant temperature ( 25 ? ? ) and 20 % soil moisture. to study the influence of inhibitors on the dynamic process of transformation and variation of availability of cd in soil, laboratory cultured soil was sampled in certain interval during the 128 - day cultured period for the analysis of cd availability and speciation. in adsorption - desorption experiment, the tested soil had fistly been cultured under constant temperature ( 25 1 ) and 20 % soil moisture for two months after the treatment according to the designed plan, then isothermal adsorption - desorption experiment was proceeded when the reaction between inhibitor and soil tended to be stable

    石灰施用量對植株吸收鍋有顯著影響，植株各部位鍋含量k ）與石灰添加量… ， ）的關系可以很好地用方程y叫（ ax斗bx化）描述，石灰對植株鍋累積的調控存在一個最佳抑制效應劑量，低於或高於這一劑量均不能達到最佳抑制效果，但對于不同植株部位和不同污染水平，最佳抑制效應劑量不同；低用量下，單位石灰添加量對植株吸收鍋的抑制作用比高用量時大，因此若綜合考慮調控劑的施用成本及其對生物量的影響效應，則在本試驗條件下以施用石灰0
  14. With the purpose of better understanding some weak links in this subject, we comprehensively and systematically compared the effects of some typical inhibitors and their different combined ways in inhibiting cd bioavailability in purple soil, further studied the laws and affecting factors of cd ' s transfer and transformation in soil - plant system, revealed the mechanisms inhibitors function, and explored the practicable inhibitor prescriptions and their usage. the results are expected to provide reliable techniques for controlling cd pollution of agricultural soils in chongqing and it is also significant to ameliorate soils polluted by other kinds of heavy metals. comprehensive methods including pot experiment, laboratory culture experiment, kinetic and thermodynamic adsorption - desorption experiment were adopted in this study, and the acidified purple soil was chosen to test, considering that cd is highly active in this soil

    本研究針對重慶市農業土壤的污染狀況，選取鎘活性較高的酸化紫色土為供試土壤，採用盆栽試驗、培養試驗、化學熱力學與動力學吸附試驗相結合的方法，系統研究和比較多種調控劑及其不同組合方式對鎘生物活性的效應，並揭示鎘在土壤?植物系統中的遷移轉化規律及影響因素，進一步分析調控劑的作用機理，探尋具有實際應用價值的調控劑配方及合理使用的方法，這不僅可以為重慶市農業土壤鎘污染的防治提供可靠的技術保障，而且對于其它重金屬污染的治理也有一定的理論意義。
  15. While considerable work has been done for the effects on crops jess work was conducted for the effects on soil quality, especially soil environment in rhizosphere. the objectives of this study were, therefor to discribe the characteristics and effects of applying re on soil mineral nutrition with a soil culture experiment as well as the effects of re on translocation and uptake of mineral ions in rhizosphere with a simulating rhizosphere experiment

    本研究以油菜和水稻為供試材料，通過土壤培養試驗和根際模擬試驗並結合室內分析研究稀土對土壤礦質營養的作用及對根際礦質離子遷移、吸收的影響，以期從根? ?土系統出發，為稀土農用增產機理和環境風險性評價提供理論參考依據。
  16. Our aim is to provide reference data for the mechanism of the effects of ree on crops and the safety evaluation of re used in agriculture. 1. in soil culture experiment, re applications with appropriate dose in dry condition increased the contents of soil available n, whose no - effect value were 505mg / kg at eighth week, whereas higher levels of re had negative effects, re applications with both appropriate and higher dose in inundated condition reduced the contents of soil nh / - n and hydrolysable n. re treatment concentrations showed significant positive correlation with the contents of soil exchangeable re. and significant negative correlation with the contents of soil available p and soil ph value. there was no significant effects of re on soil exchangeable cations, but applications of re increased the contents of soil water soluble cations, especially that of ca2 * and mg2 +

    兩種培養條件下，稀土處理濃度與土壤交換態稀土含量成正比，與有效磷含量、土壤ph成反比，對土壤交換態鹽基離子含量無顯著影響，但通過置換作用增加了水溶態離子，尤其是ca ~ （ 2 + ） 、 mg ~ （ 2 + ）離子的含量；第二周稀土處理增加交換態zn含量，對交換態fe有降低作用，旱培條件下增加交換態mn含量，淹水條件下對其有降低作用；第八周稀土處理均提高交換態fe 、 mn 、 zn含量，其機制主要與稀土降低土壤ph有關。
  17. However, the movement and transformation of n in soil under fertigation remains unclear. the methods of simulation experiment and culture experiment were used to study the movement and transformation of n in soil under fertigation by drip irrigation. the main experimental results were shown as folio wings : ( 1 ) the water infiltration under drop irrigation is a point infiltration ; and the soil moisture in vertical and horizontal directions was increased with the irrigation time, and the movement rate were decreased with the irrigation time

    本研究採用室內模擬試驗和生物培養試驗相結合的方法，研究了在滴灌施肥條件下，化學氮肥施入土壤后的遷移、轉化規律以及對作物生長的效應，獲得了以下主要結論： （ 1 ）滴灌條件下水分以點源入滲土壤，水平和垂向的濕潤鋒均隨入滲時間的增加而逐漸變大，在入滲開始階段濕潤鋒的推進速率較大，隨入滲時間的延長，濕潤鋒的推進速率逐漸變慢。
  18. 2. in rhizosphere culture experiment, re applications with appropriate dose in three weeks significantly increased the biomass ( dry matter ) of rape and rice, and the effects of re on the rape was better than the rice, but they decreased or became little significant in eight weeks

    根際培養條件下，第三周低濃度稀土處理顯著提高油菜和水稻的生物量（以干物重計） ，對油菜的作用效果優於水稻，第八周作用趨緩或不明顯。
  19. A pot culture experiment was carried out to study the effect of different phosphoric fertilizer rates on sucrose content and agronomic traits of winter wheat

    摘要利用盆栽試驗，研究不同供磷水平對小麥植株體內無機磷與蔗糖含量及經濟性狀的影響。
  20. First, the dynamics of soil properties in different period during a year from the long - term no - tillage and ridge culture experiment field are researched in order to get the general dynamic properties of organic matter, chemical substances, microbe, ph and eh in soil

    首先通過對自然免耕定位試驗的有機殘體的分解與轉化的動態特徵研究，得出有機殘體分解轉化過程中的有機質、養分、微生物及出和eh的動態特徵，總結出有機殘體分解與微生物關系的一般規律。
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