culture gene 中文意思是什麼
culture gene
解釋
文化基因-
A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv
以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。 -
Sds - page results showed that as to mut + recombinant highest yield was obtained after 4 days inducing and with the culture time prolonged it reduced. pokeweed antiviral protein gene expressed well when methanol concentration reached 10g / l. pokeweed antiviral protein obtained high yield in thin acidic culture medium ( ph6. 0 - 6. 4 ) and its quantity in total mass of secrete protein exceeded 30 %
Sds - page分析結果表明， mut ~ +組菌株在甲醇誘導第四天後pap在培養液中積累量達到最高水平，延長培養時間會導致產量下降；在10g / l的甲醇濃度誘導下， pap的表達量達到最高；培養基ph值在偏酸性條件下（ 6 . 0 - 6 . 4 ） pap的表達量都維持在較高的水平。 -
The supernate of virus culture was used as templet in overlapping pcr, then the interesting gene was obtained
利用重組pcr的方法，以病毒培養液上清為模板，把二個目的基因串聯在一起。 -
In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not
合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式（ nested ） rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl （ 590 ， 1110 ）酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。 -
On identification and analysis of school culture gene
略論校園文化基因的識別與分析 -
On the basis of reviewing on the research on organization dna and applications of gene, this paper define the organization culture gene, and demostrate on its distinguishing
在回顧企業dna研究應用的基礎上，提出並界定了企業文化基因概念，對企業文化基因識別進行了實證研究。 -
An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined
將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上，構建了4個全長基因的分子克隆，分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ，其中兩個轉移到低拷貝載體plg338上，命名為plgd30343 、 plgd70344 。 -
We used sds - page and western blot to detect a - 1b glyco - protein moleculers in culture medium. one bind was detected at mole - culer mass between 66 - 43 kda, which show a maximal level at 7 day. discussion the function of human a - 1b glycoprotein precursor gene is still not known
我們利用生長激素（ gh ）處理肝癌bel 7442細胞系， a ibgp的表達明顯高於對照組，說明gh可以調節a ibgp的表達，該基因可能參與gh的生理功能及在與gh相關的疾病中發揮作用。 -
4. after having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time, incubation time and co - culture time, we set up the system of high frequency transformation of tomato cotyledons. then hbmp - 3m gene was transferred into tomato via agrobacterium - mqdiated transformation, and the resistant plants to hyg were obtained. by pcr analysis on part of the putative transformants, we identified that hbmp - 3m gene had been integrated into the genome of part of tomato plants. 5. transferred hbmp - 3 gene into tobacco via agrobacterium - mediated transformation and obtained the resistant plants to hyg. trans genie tobacco plants were confirmed by instantaneous expression of gus gene in calli detection, growth and bio - morphology analysis, hyg - resistant experiment and pcr analysis
通過pcr檢測證實部分番茄抗性植株中已導入hbmp - 3m基因；人骨形成蛋白一3成熟膚基因和全長基因分別轉化番茄和煙草的研究5 .通過農桿菌介導法將hbmp一3全長基因導入煙草，並且獲得了hyg抗性植株，通過gus基因瞬時表達檢測、轉化植株的生長情況及形態學分析、 hyg抗性鑒定及pc尺檢測，證明目的基因己經整合到煙草基因組中。 -
Landscape gene atlas : a new angle to study the zoning of settlement culture landscape
聚落文化景觀區系研究的一種新視角 -
In this paper, the whole process of it microsporogenesis and male gametophytes development was observed with microscope to sure weather stamen development is normal. at the same time, in order to provide techniques on biotechnology conservation and the foundation of its resources gene pool in cell engineering, its techniques on culture in vitro was studied
本論文通過對蝟實小孢子發生和雄配子體發育全過程進行細胞觀察，探尋蝟實的雄性器官的發育是否是蝟實有性生殖的薄弱環節，並對蝟實的離體培養進行了初步的研究，為蝟實生物技術保存、建立蝟實種質資源基因庫提供細胞工程方面的途徑和技術。 -
In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure
我們實驗室曾用一特殊的突變檢測系統，直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變：首先用低濃度（ 0 . 2 m ）的短壽烷化劑mnng （半壽期為1 . 1hr ）處理細胞2 . 5h后，繼續培養24h ，將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制，發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。 -
Culture supernatants of bt strains induced luminescence in v. harveyi reporter strain bb170, which can only response to ai - 2 signal moleculars, indicating that the bt strains have the luxs gene. by aligning the nucleotide sequences of the b. anthracis and b. cereus luxs genes, a pair of primers were designed and an 474 - bp fragment was amplified from bt strains by pcr
首先通過生物發光實驗檢測到蘇雲金芽孢桿菌（ bacillusthuringiensis ， bt ）的培養液可以誘導特異性檢測ai - 2信號分子的哈氏弧菌報告菌株bb170發光，表明bt中含有群體感應信號分子ai - 2的合成基因luxs 。 -
Studies on culture of insect - resistant rice by transforming rice with pin gene via the pollen - tube pathway
基因通過花粉管通道法轉化水稻的研究 -
Tissue culture, gene research equipment
組織培養，基因研究設備 -
No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract
將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。 -
Expression in vitro cos - 7 cells were transfected with pm, pms, pmi and pmsi constructs by cationic liposom, respectively. 48 hours later, mrna of targets gene were detected by rt - pcr and hil - 12 protein in culture supernatnant and cell lysates were detected by western blotting. p815 cells were transfected with pm and pms constructs and selected by g418
2 .重組質粒在真核細胞中的表達: （ 1 ） pm 、 pms轉染cos一7細胞， 48小時后，用ri 』 - pcr檢測目的基因在mrna水平的表達;轉染p815細胞， g418篩選抗性細胞克隆，用rt - pcr檢測目的基因在mrna水平的表達，結果為陽性，說明在轉錄水平有目的基因的表達。 -
Gene had been highly expressed after incubating the transformed strains in the inducing culture
基因已被轉化，誘導培養后的蛋白電泳顯示 -
In this project, based on a great quantity of screening experiments, b. thuringiensis and b. cereus strains " culture was found containing a kind of protein which inhibits autoinducer activity. this protein was named aii and its encoding gene was named aii
本研究經大量篩選，發現蘇雲金芽胞桿菌和蠟狀芽胞桿菌的培養物可抑制酰基高絲氨酸內酯（ acyl - homoserine - lactone ，簡稱ahl ）類ai分子的活性，其活性物質是一種蛋白質，稱之為aii蛋白。 -
To inestigate the long - term culture conditions of bone marrow stromal cells, their efficacy of expressing exogenous gene and their ability to differentiate into adipocytes
研究骨髓間充質細胞的體外長期培養下外源基因的表達和向脂肪細胞分化的能力。
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