cultured sample 中文意思是什麼
cultured sample
解釋
培養樣品-
Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。 -
By sun, bo ( major : marine biology ) directed by professor zhang, guofan and dr. liu, xiao population genetics of four argopecten irradians cultured populations was analyzed by using random amplification polymorphism dna ( rapd ) techniques. and partial dna sequences of the internal transcribed spacers ( its ) from four representative samples ( one sample represent for each population ) were amplified by pcr and sequenced in order to stress the phylogenetic relationships of the four populations. besides, by rapd technique, two argopecten irradians families were studied to examine whether the hybridization experiments were successful and which family was more suitable to be materials in future genetic linkage map construction
本文以海灣扇貝4個養殖群體為研究對象，採用隨機引物多態性dna （ rapd ）技術進行了群體遺傳學研究，並嘗試使用4個群體中代表個體的內轉錄間隔區（ its ）序列比對分析結果作為參考；另外，本文還運用rapd技術對人工異體交配的2個海灣扇貝家系的雜交成功率進行了分析，均獲得成功證實，並評估了兩個家系構建遺傳連鎖圖譜的潛力。 -
Influenza virus sample in cultured cells was treated for reverse transcription, amplification, fluorescent dye labelling, hybridization and scanning
對待檢樣品進行隨機逆轉錄和擴增，熒光標記，雜交，掃描分析。
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