cytopathogenic 中文意思是什麼
cytopathogenic
解釋
細胞病原性-
The bvdv nadl strain is cytopathogenic. searching fromgenbank several cp strains " sequence of e2 gene about deer - n21, sh9, newyork - i and so on were found. according to the homologous sequence they were designed and synthesised a pair of primers by the biology software primer 5 and added bal i and nco i site to the 5 " end which can be used for the application of e2 gene and subcloning
從genbank中搜尋出deer - n _ （ 21 ） 、 sh9 、 newyork -等cp型毒株的e _ 2基因序列，根據其同源性用生物學軟體primer5設計引物，並在引物的兩端加入bal和nco兩個酶切位點，酶切位點的存在易於對e _ 2基因進行克隆操作。 -
Compared to its parental virus bartha - k61 strain, it displayed no obvious differences in virus multiplication and cytopathogenic effects in different cell cultures ( pk - 15, ibrs2, vero and cef )
在不同的細胞上（ pk - 15 、 ibrs2 、 vero和cef ）對重組病毒的病毒滴度和病變與親本毒進行比較，未發現顯著差異。 -
After obvious cytopathogenic effects developed, virus - contained supematants were harvested, and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal. single blue plaques were picked, and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification. the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain
該載體與具有高度感染性的bartha - k61株基因組dna通過脂質體加plus法共轉染vero細胞，採用甲基纖維素固定病變， x - gal染色，經過10代藍斑純化獲得了一株穩定表達lacz基因的ge tk基因缺失突變株，命名為rprv - lacz 。
分享友人