czapek medium 中文意思是什麼
czapek medium
解釋
恰佩克培養基-
These vectors were tried to transform into the p. digitatum imazalil - sensitive isolate by the methods of peg - mediated or electroporation transformation. after that, the transformed protoplasts were regenerated on czapek medium containing hygromycin at 300 # g / ml. unfortunately, the ideal transformants could not be obtained
採用peg法和電擊法兩種方法進行指狀青黴原生質體的轉化，然後將得到的轉化產物轉移到查氏再生培養基中培養，遺憾的是沒有得到理想的轉化子。 -
The effect of some factors, including the appropriate materials for isolating protoplasts, the concentrations of enzyme, period of digestion and temperatures, and osmotic pressure stabilizers on the isolation and regeneration of protoplasts in penicillium digitatum were studied. the results demonstrated that the purified protoplasts could regenerate through double layers of czapek medium containing 0. 7mol / l nacl. the regeneration rate could reach 24. 9 %
通過對制備材料、酶液濃度、酶解時間、酶解溫度、滲透壓穩定劑的種類和濃度等因素的實驗研究，得到了一套制備指狀青黴（ penicilliumdigitatum ）原生質體的有效方法，並在雙層培養基上初步實現了原生質體的再生，再生率可達24 . 9 。
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