degenerate region 中文意思是什麼

degenerate region 解釋
簡並區
  • degenerate : vi 1 腐化，墮落，頹廢。2 衰敗；【生物學】退化 （to）；【生理】 變質。adj n 1 腐化的，墮落的，頹廢...
  • region : n. 1. 地方，地域，地帶；地區；行政區，管轄區，區；左近，鄰近；（大氣、海水等的）層，界，境。2. 【解剖學；動物學】（身體的）局部，部位。3. （學問等的）范圍，領域。4. 〈罕用語〉天空。
  1. Using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, tow different fragments of sox gene, essox3 and essox22 were amplified from female and male eriocheir sinensis, the sequence results indicated that essox3 and essox22 shown high homology to human sox genes, and the identities to human sox genes in dna sequence and amino acid sequence are 84 % 、 85 % and 97 % 、 81 %, respectively. it might be concluded that sox gene was highly conservative in phylogenesis

    二、研究論文1 、參照人sry基因hmg - box保守區的序列，設計一對兼并引物， pcr擴增了中華絨螯蟹的sox基因，並對擴增產物進行了克隆和測序。結果在雌雄個體中篩選出兩個不同的sox基因essox3和essox22 ，其dna序列和編碼的氨基酸序列與人相應sox基因的相似性分別為84 % 、 85 %和97 % 、 81 % ，顯示該基因在進化上具高度的保守性。
  2. 2. using a pair of degenerate primers based on the conserved region, hmg - box, of human sry gene, eight different fragments were amplified from both female and male rana rugulosa wiegmann then cloned by using pmd18 - t vector and sequenced

    2 、參照人sox基因設計了一對兼并引物，擴增了虎紋蛙的sox基因，並對擴增產物進行了克隆和測序。
  3. The results demonstrate that the upper limit of permafrost in this area had declined from 1970s to the middle phase of 1990, and permafrost has appeared degenerate tendecy, but since middle 1990s, permafrost have a stable state ; ground temperature of roadbed near the earth surface is higher than that of the natural ground, thawing time of roadbed near the earth surface is longer than that of the natural ground, the heat income within the permafrost is greater than the heat release from the permafrost during anannual period, the heat accumulation within the permafrost is temporarily appeared as temperature rising, but with the heat accumulation within permafrost growing up year by year, permafrost temperature will become higher gradually, and strong thawing of permafrost could be happened in the region

    結果表明：風火山地區從20世紀70年代到90年代中期凍土上限下降，凍土出現退化現象，從90年代至今凍土趨于穩定；路基近地表地溫明顯高於對應天然地表下的地溫，路基近地表經歷的融化期長于對應天然地表，進入多年凍土區的熱收支也呈現出吸熱明顯大於放熱的周期性變化，進入多年凍土的熱積累暫時以增高地溫耗熱為主，但隨著凍土吸熱量的逐年積累、凍土溫度的不斷升高，本區凍土可能發生強烈融化。
  4. A pair of degenerate primers were designed in the conserved domain which based on the alignment of acbf gene family in tobacco and arabidopsis. a 239 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ), which was used as a probe for screening tomato fruit ( pink stage ) cdna library. one positive clone containing entire coding region were isolated, which was identified as a new member of acbf family by blast server, and named as leacbf

    根據genbank （中的擬南芥、煙草acbf家族成員序列比較的結果，在該基因的保守區設計簡並引物（ degenerateprimer ） ，以轉色期普通番茄果實的rna為模板，進行rt - pcr擴增，獲得239bp的擴增片段，以此片段作為探針篩選轉色期普通番茄果實cdna噬菌體文庫，獲得了包含全長編碼區的陽性克隆。
  5. With a pair of degenerate primers designed according to the conserved region among the known cdna sequences of five rodents, a cdna fragment was generated by rt - pcr from total rna isolated from ovaries of lagurus lagurus

    首先根據已發表的五種嚙齒目動物zp3cdna序列同源性分析結果設計特異性引物， rt - pcr擴增草原兔尾鼠zp3 （ lzp3 ） cdna保守序列。
  6. Part two : studies ; l. the sox gene of dinodon refozonatum was amplified by using a pair of primers which can amplify the conservative motif ( hmg - box ) of human sry gene. the amplification band was observed in both male and female dinodon refozonatum, whose length was consistent with that of human sry gene, which about 220bp. the result of sscp analysis showed that there were many differences in the sox gene sequence between dinodon refozonatum and human, and there was a few differences between male and female dinodon refozonatum. 2. using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, six different fragments were amplified from either female or male dinodon refozonatum, then cloned by using pmd18 - t vector and sequenced

    結果顯示： （ 1 ）赤鏈蛇基因的擴增片段與人sry基因擴增片斷大小相同，為220bp左右； （ 2 ）雌、雄赤鏈蛇sox基因的擴增片段大小雖然與人的相同，但其單鏈遷移率與人的有較大差異，而且雌雄個體間有明顯差異，預示該基因的dna序列在雌雄個體中可能有差異； 2 、參照人sry基因hmg - box保守區的序列，又設計一對兼并引物，擴增了赤鏈蛇的sox基因，並對擴增產物進行了克隆和測序。
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