denaturing gel 中文意思是什麼
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Application of denaturing gradient gel electrophoresis in microbial ecology
在微生物生態學中的應用 -
Analyse the research of distributing of microbe community and the tendency of the change, disscuss the principle and traits of denaturing gradient gel electrophoresisand terminal restriction fragment length polymorphism, to research the law of change that the microbe community have in composting process, we can get effective and rapid information to filtrate the microorganism during composting process, then accelerate the development of compost technology
摘要對堆肥微生物種群分佈及其動態變化的研究進行了分析，論述了分子生物技術中的變性梯度凝膠電泳和末端標記限制性片段長度多態性的原理和特點，以及用於研究堆肥微生物的群落結構演變規律，為分析和篩選堆肥中的微生物提供更加有效、快速的信息，促進堆肥技術的發展。 -
Pcr - based analysis method was carried out in the following study. dna amplification by pcr in cgg repeats were performed on 154 people as normal control ( 73 males and 81 females ) and 23 members from four x - linked mental retardation ( xlmr ) families. after electrophoresed on a 6 % - denaturing polyacrylamide gel ( acr : bis = 19 : l ), genotypes of the objects were determined by analyzing pcr products
本研究採用較以往更為簡便有效的pcr技術方法，對隨機抽取的154人的正常群體（男性73人、女性sl人）及來自四個智力低下家系的23名成員進行了fmr基因cgg重復序列的擴增，利用變性聚丙烯酸胺凝膠電泳技術對不同等位基因類型進行分離和統計。 -
The pcr amplification products were analyzed by vertical denaturing polyacrylamide gel electrophoresis followed by silver staining
Ys393二基因座， pag電泳分型，銀染顯色。 -
The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100. the sod activity for purified enzyme could reach 4966 iu / mg proteins, and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis. when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0. 2mmol / l kcn or 0. 5 mmol / l h2o2, the active band was still showed at the same position, which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod
通過sds一聚丙烯酸胺凝膠電泳可知該sod酶的分子量約為20kda .在非變性聚丙烯酞胺凝膠（ pagb ）電泳后，在凝膠son顯色反應液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝膠sod顯色反應后在原來的sod酶部位仍然含有sod活性帶，這表明利用kcn和h20 :處理並不能抑制500的活性，該sod屬于mn一sod 。 -
Protocols of applification of denaturing gradient gel electrophoresis in studies of environmental microorganism
變性梯度凝膠電泳在環境微生物研究中的應用詳解
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