densitometric analysis 中文意思是什麼

densitometric analysis 解釋
光密度分析
  • analysis : n. （pl. -ses ）1. 分解，分析；【數學】解析。2. 梗概，要略。3. 〈美國〉用精神分析法治療（＝ psychoanalysis）。
  1. Ptxb1 - hng and ptxb1 - m - insulin are expressed in e. coli successfully. after sds - page and densitometric scan analysis, the results show that the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 % of total bacterial proteins. western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody ( igg )

    Pbv220 ? hng在大腸桿菌中未檢測到表達，后兩個克隆在大腸桿菌bl21 （ de3 ）中獲得高效表達， hng及m - insulin融合蛋白表達量分別佔全菌蛋白的40及50左右；經western - blot鑒定m - insulin融合蛋白可以與小鼠抗人胰島素單克隆抗體（ igg ）發生抗原抗體結合反應。
  2. Conclusion we have constructed the expression plasmid pbv220 - hpf4 successfully. 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa by pcr. after sds - page and densitometric scan analysis, the expression level of r hpf4 is 25 - 30 %

    結論本研究運用pcr定點突變技術，完全去除了hpf4cdna基因3 」端utrat富含區：改用大腸桿菌強串聯終止密碼子taaaataa ，成功構建高效表達克隆pbv220 rhpp4 。
  3. After sds - page and densitometric scan analysis, the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 %. western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody, we got hng fusion protein and m - insulin fusion protein with purity of above 80 %

    今士考個二目卜乙成功構建了ptxbi一hng及ptxbi一m一insulin原核表達克隆，並獲得了高效表達，經過純化得到純度人於80 %的融合蛋白，並對人胰島素突變體融合蛋白進行了初步活性測定。
  4. After washing with reagent, adopt the newest purification technology source30rpc, sds - page and densitometric scan analysis, the result show that expression level is 90 % of total bacterial proteins. after renaturation, ifnr, hgfa, hgfb, hpk5 were purified by akta purifier chromatogram instrument, sepharose fast flow, ssphacrayl series gel, selecting optimize condition. finally establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies, purification product purity > 98 %

    結論:總之，通過對發酵罐中重組工程菌各種培養因素的研究，建立了一種高密度、高表達發酵工藝體系，為重組蛋白的后續純化提供了大量、穩定的原料供應;通過對不同目的蛋白的色譜行為的系統研究，建立了一種高效穩定、快速簡潔、易於放大的包涵體重組蛋白分離純化體系。
  5. 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa. 2 after sds - page and densitometric scan analysis, the result show that expression level is 25 - 30 % of total bacterial proteins

    山西醫科大學2002屆碩士學位論文一2dhsa pbv220 rhpf4經溫控誘導表達后， sds page及凝膠密度掃描分析，表達產物占總國體蛋白的25 30 ，凝膠遷移特性與hpf4標準品相同。
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