digestion cell 中文意思是什麼

digestion cell 解釋
消化槽
  • digestion : n. 1. 消化；消化力，消化作用。2. （精神上的）同化吸收，融會貫通。3. 【化學】浸煮（作用），浸提。4. 菌致分解〈用細菌分解法處理污水〉。
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  1. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  2. In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks

    方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。
  3. The organization cuts into slices and examines by the in situ pcr, drip protease k 20 ( xl with loomg / ml to digest respectively in pretreatment, increase with normal position positive cell account for total ratio of cell, according to the positive standard cells > 75 %, confirm the lightest digestion time, studying the influence and relationship of different fixation time with protease digesting each other, detecting the mn genotype of the organize slices at the same time

    石蠟切片進行原位pcr檢，預處理分別滴加loom歲血的蛋白酶k20閃消化，以原位擴增顯色后陽性細胞占總細胞的比值> 75 %為標準，確定最適消化時間『 ， ，研究不同固定時間與蛋白酶消化的相互影響和關系，同時檢測石蠟切片的mn基因型。
  4. Most commonly used is the mechanical method in which a small piece of testicular tissue is torn apart using needles and the spermatids are released. while enzymatic digestion of human testicular tissue can result in a mixture of cell types including a whole range of spermatogenic cell types

    最常用的是機械方法，即利用細針將活檢獲得的小塊睪丸組織撕開，釋放出精子細胞；也可利用酶將睪丸組織消化成細胞懸液，其中包含所有類型的生精細胞。
  5. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  6. The recombinant was identified by dual enzyme digestion and the direction of cd40 / pcdna3 was analysed with t7 primer. after being packed by lipofectaminetm2000, the recombinant was transfected into b lymphocytes. cd40 expression on membrane, cell proliferation and antibodies concentration were detected with flowcytometry, mtt colorimety assay and el1sa, respectively

    以脂質體為介質瞬時轉染健康人及sle患者b細胞系，利用流式細胞技術檢測膜cd40的表達情況，並利用mtt比色法檢測細胞的增殖能力， elisa法檢測培養液的ig濃度，以研究b細胞在cd40被抑制以後增殖能力、抗體分泌的改變。
  7. Protoplast the protoplasm and plasma membrane of a cell after removal of the cell wall, where present. this can be achieved by physical means or by enzymic digestion

    原生質體：指去掉細胞壁以後的細胞原生質以及質膜。可用物理方法或酶消化得到。
  8. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  9. All study aim is to lay a foundation for clinic appliance. methods : 1 ) hpfl was isolated by enzymatic digestion derived from rat fetal liver on ed13. 5d. furthermore, erythrocyte and other cell were removed from hpfl by erythrocyte - cracking solution and different attachment method

    研究方法： 1 ）取ed13 . 5d的大鼠胎肝，酶消化法離散細胞，用紅細胞裂解液去除紅細胞，差速貼壁法去除其它細胞，接種于不同的基質和培養液中， mtt法比較不同培養液和培養基質對大鼠hpfl的體外生長影響。
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