digestion trial 中文意思是什麼
digestion trial
解釋
消化試驗-
In the first trial, combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis, and then a modified discontinuous percoll gradient centrifugation method ( 15 %, 22 %, 30 %, 40 %, 50 %, 60 % ) was introduced to isolate spermatids from the cellular suspensions. the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis, and the viability of spermatogenic cells was assessed by using eosin y exclusion test
在第一部分試驗中,首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液,然後經6層非連續percoll梯度離心法( 15 、 22 、 30 、 40 、 50和60 )分離,通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量,並以伊紅y排斥試驗測定細胞的存活率。
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