dna antibody 中文意思是什麼

dna antibody 解釋
脫氧核糖核酸抗體
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • antibody : n. 【醫學】抗體。
  1. This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting

    本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基
  2. We immunize the balb / c mice with pc4. 0f and pc3. 1f by paunch and muscle administration and immunize each with different content of 10 g, 50 g 100 g. the antibodies in serum against newscastle disease virus f gene protein are tested by elisa. the result showes that two kinds of dna vaccines can induce specific antibody response to encoded proteins in 15 weeks by im and ip, but antibodies response is little different

    免疫結果顯示，採用2種免疫途徑（肌肉注射，腹腔注射） ， 3個劑量（ 10 g 、 50 g和100 g ）免疫balb c小鼠，在被檢測的15周內，肌肉注射和腹腔注射組均可產生抗體，抗體的產生有明顯的劑量依賴性， 50 g只和100 g只明顯優於10 g只，而100 g只與50 g只相似（肌肉注射）甚至稍低（腹腔注射） 。
  3. Frankfurt os, robb ja, sugar baker ev, et al. monoclonal antibody tosingle2stranded dna is specific and sensitive cellular marker of apoptosis [ j. exp cell res, 1996, 226 ( 2 ) : 3872397

    吳國慶，李少華，徐志偉，等.抗單鏈單克隆抗體雜交瘤細胞株的建立及鑒定[ j ] .細胞與分子免疫學雜志， 2002 ， 18 （ 5 ） : 4792480
  4. The diagnostic value of associated detection of anti - tuberculosis antibody, dna and tuberculin test in pulmonary tuberculosis with negative spuntumcultivations

    三種方法聯合檢測對菌陰肺結核的診斷價值
  5. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  6. The 7mer peptide and 12 mer peptide selected from the peptide library elicited strong anti - vegf antibody immuno - reaction in mice. dna sequenseing showed that gwyydal and vasavfysalve mimic the discontinuous epitope of vegf by the 14 - 17, 21 and 21 25 amino acid residues respectively

    兩者可能分別模擬了vegf分子的21 、 25位氨基酸和14 、 17 、 21位氨基酸通過折疊后在三級結構水平上形成的結合位點和vegf競爭結合受體。
  7. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  8. It underlies the action of hormones, the control of dna transcription, the recognition of antigens - antibody in the immune system, the catalysis of chemical reactions by enzymes and the actions of many drugs

    例如dna的轉錄，激素的作用機制，免疫系統中的抗原?抗體識別，化學中的酶催化反應，以及很多藥物發揮藥效的機制都與之密切相關。
  9. In the present paper the application of dna library, phage display random peptide library, phage antibody library, etc. in the research of serodiagnostic reagents and their special values were reviewed

    本文綜述了基因文庫、噬菌體展示肽庫及噬菌體抗體庫技術在血清學診斷試劑研製中的應用及其各自的優點。
  10. Hisityl - trna synthetase catalyzes the aminoacylation of trnahis in the initial step of protein biosynthesis. the involumen of histidyl - trna synthetase in autoimmune diseases is another feature of the enzyme. in studies, it is reported that purified jo - 1 antigen can increase the detection rate of anti - jo - 1 antibody. but it is difficult to obtain a single component by biochemical extraction. genetic engineering can help us to desolve this problem. after looking up mrna sequence encoding histidyl - trna synthetase in genebank, we used rt - pcr technology to gain its full length dna sequence. the vestor ptybllwas used in the construction of expressing vestor. we transformed the jo - 1 gene into er2566 and used this system to express fusion jo - 1 antigen

    本實驗從人胎盤中提取總rna ，利用rt - pcr技術獲得了編碼jo - 1的基因整長序列，選用impact - cn系統中的ptyb11載體，構建了jo - 1基因的克隆與表達載體，並轉化大腸桿菌er2566 ，經過抗性篩選、分子量大小比較、雙酶切鑒定、和pcr鑒定等多種方法驗證，篩選出了5個陽性克隆。
  11. The fusion ( f ) protein and hemagglutinin - neuraminidase ( hn ) are the major virulence factors of the virus to which the host responds well with protective antibody production. recombinant plasmids containing the f or hn gene can induce specific immune responses against ndv. however, dna vaccination by injection or gene gun routes is rather inconvenient and costly in field applications in scaled animal farms

    融合蛋白（ f ）和血凝素-神經氨酸酶蛋白（ hn ）是ndv的主要宿主保護性抗原，以f基因和hn基因為免疫原研製的dna疫苗能誘導一定程度的免疫應答，但注射免疫或基因槍免疫途徑因實際操作和成本等問題難以推廣應用，而直介面服重組質粒dna免疫效果不理想。
  12. Constructed the fusion protein expression vector of echistatin, . and identify it by dna sequencing. the vector can express echistatin fusion protein at a high level, the fusion protein molecular weight is about 16000 da in sds - page analysis, and the fusion protein consists more than 30 % of total protein, the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody. the fusion protein include : hpk5. his + 6tag, and echistatin. echistatin can be released by cnbr cleavage

    構建了echistatin融合蛋白表達載體，經dna測序鑒定正確后，溫控誘導表達，獲得了高效表達，表達產物經505一隊ge分析，分子量為16000oa ，符合預計的融合蛋白分子量，表達產物. ll 』菌體總蛋白的30 %以上， westem一blotting結果顯示，該日的蛋白能夠和echistatin多克隆抗體發生特異性抗原抗體反應，表明我們成功構建了eohistatin的高效融合表達克隆。
  13. 2 specific epitope analysis in m3m4 loop target to determine the b cell epitope of a monoclonal antibody against the m3 - m4 loop of nmdar1, a random phage displayed dodecapeptide library was screened with the monoclonal antibody mab363 against the m3m4 loop of nmdar1. after four rounds of biopanning, the peptide sequences of positive phage clones were determined and analyzed by dna sequencing

    流式細胞儀測定脾細胞cd4 + 、 cds寸淋巴細胞亞群，間接elisa測定血清thl細胞因子幾一2 、 tn下一a和n一y ， th一2細胞因子幾一4 、 il巧和幾一6的水平，同時檢測特異性抗體產生情況。
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